Effects of sulfasalazme on biopsy mucosal pathologies and histological grading of patients with active ulcerative colitis

AIM： To investigate the mechanisms of sulfasalazine （SASP） in the treatment of ulcerative colitis （UC）.METHODS： Changes of pathological signs and histological grading of 106 patients with active UC were observed before and after the treatment with SASP, 1 g, thrice daily for 6 wk.RESULTS： The effect of SASP on the vasculitis in lamina propria was 48.2% and 17.4% in the mild active UC （P〈0.001） and 68% and 26.7% in the moderate active UC （P〈0.001） before and after treatment, Fibroid necrosis of vessel wail was found in one case of mild UC and two cases of moderate UC before treatment and was not found after treatment, No thrombosis was found in mild UC before and after treatment, while thrombosis was found in one case of moderate UC before treatment, The effect on mucosal glandular abnormality was 30.4% and 13.0% in mild UC （P〈0.05）, and 42% and 40% in moderate UC （P〉0.05） before and after treatment, The rate of eosinophil infiltration was 98.2% and 80,4% in mild UC （P〈0.01）,and 100% and 91,1% in moderate UC （P〈0.05） before and after treatment, The effect on crypt abscess was 21.4% and 4.4% in mild UC （P〈0.05）, and 48% and 13.3% in moderate UC （P〈0.001） before and after treatment. The effect on mucosal pathohistological grading was 2.00±0.84 and 0.91±0.46 in mild UC （P〈0.001）, and 2.49±0.84 and 1.31±0.75 in moderate UC （P〈0.001） before and after treatment.CONCLUSION： SASP can improve small vessel lesions and crypt abscesses and reduce neutrophilic and eosinophilic leukocyte infiltration in inflammatory mucosa of UC.

Relationships of tumor inflammatory infiltration and necrosis with microsatellite instability in colorectal cancers

AIM:The relationships between microsatellite instability (MSI) and survival in colorectal cancer patients are not consistent. The favorable survival of patient with MSI has been suggested to be related to pronounced inflammatory infiltration; however,the reason for non-association of MSI with survival is unclear. Our aims were to investigate the associations of inflammatory infiltration and tumor necrosis (TIM) with microsatellite status and clinicopathological factors in colorectal cancer patients in whom MSI was not related to survival. METHODS: Three hundred and one colorectal adenocar-cinomas were evaluated for inflammatory infiltration and 300 for TN under light microscope. RESULTS: Low infiltration at invasive margin (X2=3.94, P= 0.047) and in whole tumor stroma (X2=3.89, P= 0.049) was associated with MSI, but TN was not (X2=0.10, P = 0.75). Low infiltration was related to advanced stage (X2 = 8.67, P= 0.03), poorer differentiation (X2=8.84, P= 0.03), DNA non-diploid (X2=10.04, P= 0.002), higher S-phase fraction (X2=11.30,P=0.004),positive p53 expression (X2=7.94,P=0.01),and worse survival (P = 0.03 for both univariate and multivariate analyses). Abundant TN was related to advanced stage (X2=17.74, P= 0.001) and worse survival (P= 0.02 for univariate, and P= 0.05 for multivariate analysis). CONCLUSION: The result that high inflammatory infiltration was not related to MSI might help explain the non-association of MSI with survival in colorectal cancer patients.

Increase of tumor necrosis factor receptor 1 expression in women with unexplained early spontaneous abortion

Objective: To investigate membrane tumor necrosis factor receptor 1 protein expression level in decidua and concentration of soluble tumor necrosis factor receptor 1 in serum in women with unexplained early spontaneous abortion, threatened abortion, and compare the levels with healthy pregnant women. Methods: Thirty-seven women with unexplained early spontaneous abortion, 27 women with threatened abortion, and 34 healthy pregnant women undergoing artificial abortion of pregnancy at 6 - 10 weeks of gestation were selected. Decidual samples were collected when women were undergoing artificial abortion, and blood samples were collected at the same time. The level of membrane tumor necrosis factor receptor 1 in decidua was detected by flow cytometer, and the concentration of soluble tumor necrosis factor receptor 1 in sera was measured with an enzyme-linked immunosorbent assay. Results: The percentages of membrane tumor necrosis factor receptor 1 positive decidual cells were 16.42 ± 7.10 Mean ± SD for women with unexplained early spontaneous abortion and 13. 14 ± 6.30 for healthy pregnant women ( P < 0.05). Serum concentration of soluble tumor necrosis factor receptor 1 was significantly higher in women with unexplained early spontaneous abortion than in healthy pregnant women and in women with threatened abortion, and no difference was found between healthy pregnant women and women with threatened abortion. Conclusion: Women with unexplained early spontaneous abortion present significantly higher expression of tumor necrosis factor receptor 1 than healthy pregnant women, suggesting that over-expression of tumor necrosis factor receptor 1 may contribute to the development of early spontaneous abortion.

外公家的白果树

我外公家在安陆。安陆物产丰富,我印象最深的就是安陆的白果树。房前屋后,沟壑山岗,随处可见。我外公家的门前就有一颗古老的白棵树,因树而得名,也叫白果树。白果,学名银杏,为中生代孑遗的稀有树种,系中国特产。可食用且大补,入肺经、益脾气、定喘咳、缩小便。可治疗"疮疥疽瘤、乳痈溃烂、牙齿虫龋、小儿腹泻、赤白带下、慢性淋浊、遗精遗尿等症。但多食中毒,相传贺龙在安陆一次食用过量,身体不适,卫士们大惊,以为是特务投了毒呢。外公家的白果树下,是我童年的乐园。