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痰瘀伏络是心血管疾病链的主要机制 被引量:48
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作者 邓悦 吴宗贵 +5 位作者 陈颖 姜丽红 郭家娟 常立萍 崔英子 李双娣 《中医杂志》 CSCD 北大核心 2011年第20期1733-1735,共3页
提出了心血管疾病链与痰瘀伏络的相关理论,论证了痰瘀伏络是心血管疾病链各病变环节逐级传导的内在病理基础,痰瘀伏络是心血管疾病链的主要机制,也是造成心血管疾病易反复、难治愈、预后差的根本原因。并提出以"伏邪多与痰瘀相关&q... 提出了心血管疾病链与痰瘀伏络的相关理论,论证了痰瘀伏络是心血管疾病链各病变环节逐级传导的内在病理基础,痰瘀伏络是心血管疾病链的主要机制,也是造成心血管疾病易反复、难治愈、预后差的根本原因。并提出以"伏邪多与痰瘀相关"为突破口,把握伏邪的致病特点,遵循伏邪入络的致病规律,探索早期干预心血管事件的诊疗方法,可采用益气化瘀、豁痰通络为主要治疗原则,为中医药全面干预心血管疾病链拓宽思路。 展开更多
关键词 伏邪理论 痰瘀伏络 心血管疾病链
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浅谈基于疾病发展链的重症疾病并发症编码模式 被引量:2
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作者 何毅 卢瑶 +2 位作者 黎日铭 谭文丹 蒋小花 《现代医院》 2021年第4期604-607,共4页
DRGs和公立医院绩效考核工作在二级医院的开展,使疾病编码工作的重要性进一步的提升,疾病编码不仅要求正确,也要求完整,但由于临床思维和疾病编码的要求存在一定的差异,医生使用重症、重度等词对疾病针对疾病进行修饰,特别是疾病编码2.... DRGs和公立医院绩效考核工作在二级医院的开展,使疾病编码工作的重要性进一步的提升,疾病编码不仅要求正确,也要求完整,但由于临床思维和疾病编码的要求存在一定的差异,医生使用重症、重度等词对疾病针对疾病进行修饰,特别是疾病编码2.0对部分疾病进行了细化扩展,容易在编码时忽略了疾病分类的内涵造成重症疾病的信息丢失。运用疾病发展链的观念对疾病发展过程进行检视,能够更有效的提高重症、重度疾病的编码完整性。 展开更多
关键词 疾病发展 诊断 疾病编码 并发症
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一非肌球蛋白重链9基因相关疾病家系患者的临床及分子生物学特点 被引量:1
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作者 张淑芳 张应爱 +3 位作者 王顺兰 邓湘东 肖敬川 余平 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2011年第1期109-112,I0003,共5页
目的:探讨一非肌球蛋白重链9基因相关疾病(MYH9-RD)家系的临床表型和分子生物学特性,以阐明MYH9-RD形成的分子机制。方法:通过临床评估和实验室检测对家系进行筛查,应用光学显微镜和自动血细胞计数仪对家系成员进行血小板计数及外周血... 目的:探讨一非肌球蛋白重链9基因相关疾病(MYH9-RD)家系的临床表型和分子生物学特性,以阐明MYH9-RD形成的分子机制。方法:通过临床评估和实验室检测对家系进行筛查,应用光学显微镜和自动血细胞计数仪对家系成员进行血小板计数及外周血细胞形态观察,应用聚合酶链反应和直接测序方法分析家系患者MYH9基因突变情况。结果:家系内MYH9-RD患者15例,所有患者均具有典型的"血小板减少、巨大血小板和粒细胞包涵体"三联症,而且都有轻至中度的出血倾向;临床表现具高度复杂性,并伴有严重的白血病、青光眼、心功能不全、转氨酶升高、血脂升高、哮喘、鼻炎及白内障等多种疾病;家系患者MYH9基因的所有外显子与侧翼区均未检测到致病性突变。结论:临床表现和实验室检测表明该家系MYH9-RD诊断成立,其临床表型复杂多样可能与家系患者MYH9基因的所有外显子与侧翼区未检测到致病性突变有关。 展开更多
关键词 非肌球蛋白重9基因相关疾病 临床表型 基因 突变 系谱 遗传学
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非肌性肌球蛋白重链9基因相关疾病的研究进展 被引量:3
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作者 张淑芳 张应爱 +2 位作者 王顺兰 肖敬川 余平 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2009年第3期574-578,共5页
非肌性肌球蛋白重链9基因相关疾病(MYH9-RD)是一组罕见的人类常染色体显性遗传的巨大血小板减少症,其发病机制、基因型与表型之间的相互关系等目前还不甚明确,本文作者对近年来MYH9相关疾病方面的研究做一综述。
关键词 非肌性肌球蛋白重9基因相关疾病 非肌性肌球蛋白重ⅡA 发病机制 基因型与表型
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1例非肌性肌球蛋白重链9基因相关疾病家系并文献复习 被引量:2
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作者 罗小娟 曹科 +5 位作者 陈诗杨 付笑迎 毛晓宁 张艳 李长钢 陈运生 《临床检验杂志》 CAS 2021年第10期771-775,共5页
目的鉴定1例非肌性肌球蛋白重链9(MYH9)基因相关疾病家系的致病突变。方法调查收集先证者和家系成员病史资料,观察其临床特征和实验室检查指标。采用芯片捕获高通量测序方法检测先证者MYH9基因,确定突变位点后,对先证者和家系成员进行Sa... 目的鉴定1例非肌性肌球蛋白重链9(MYH9)基因相关疾病家系的致病突变。方法调查收集先证者和家系成员病史资料,观察其临床特征和实验室检查指标。采用芯片捕获高通量测序方法检测先证者MYH9基因,确定突变位点后,对先证者和家系成员进行Sanger验证。结果该家系三代4名患者均有鼻衄、瘀斑紫癜、外伤血肿或月经量增多病史,长期存在镜下血尿和蛋白尿。血涂片镜检均有血小板减少、巨大血小板和粒细胞异常包涵体“三联征”。所有患者MYH9基因第40内含子供体剪接位点存在错义突变c.5765+2T>A(p.R1922Rfs*43),且该基因突变与疾病表型共分离。结论该家系存在MYH9基因c.5765+2T>A(p.R1922Rfs*43)剪接突变,是MYH9基因相关疾病的致病突变,为国内首次报道。 展开更多
关键词 非肌性肌球蛋白重9基因相关疾病 MYH9基因 基因突变 巨大血小板减少症
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自身炎症性疾病一免疫性疾病链新概念
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作者 曾萍(综述) 曾华松(审校) 《国际儿科学杂志》 2013年第3期282-286,共5页
自身炎症性疾病和自身免疫性疾病的概念不同又相似,随着许多免疫性疾病基因遗传背景和炎症反应通路的阐明,一些疾病的种类面临新的划分。免疫性疾病链概念的阐述,更新了一些疾病的概念,有利于从分子水平理解疾病的本质,指导治疗。
关键词 自身炎症性疾病 自身免疫性疾病 免疫性疾病链
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非肌球蛋白重链9相关疾病大家系患者中性粒细胞包含体特点分析 被引量:1
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作者 张淑芳 张应爱 +2 位作者 王顺兰 肖敬川 黄邓高 《中国实验诊断学》 2013年第1期23-26,共4页
目的分析一非肌球蛋白重链9相关疾病(MYH9-RD)大家系患者中性粒细胞包涵体的形态和结构特点,阐明MYH9-RD中性粒细胞包涵体形成的特点,以探讨MYH9-RD中性粒细胞包涵体的致病机制。方法应用光学显微镜(瑞姬染色)和电子显微镜观察中性粒细... 目的分析一非肌球蛋白重链9相关疾病(MYH9-RD)大家系患者中性粒细胞包涵体的形态和结构特点,阐明MYH9-RD中性粒细胞包涵体形成的特点,以探讨MYH9-RD中性粒细胞包涵体的致病机制。方法应用光学显微镜(瑞姬染色)和电子显微镜观察中性粒细胞包涵体形态和超微结构特点;应用间接免疫荧光复染技术观察MYH9-RD患者与正常对照者之间非肌性肌球蛋白IIA(NMMHC-IIA)在胞浆中的分布情况。结果光学显微镜和电子显微镜下可见中性粒细胞中明显的包涵体,形态多样,大多位于细胞边缘;间接免疫荧光复染技术显示患者和正常人之间NMMHC-IIA在中性粒细胞中的分布有差异,中性粒细胞胞浆中存在明显绿色荧光的包涵体,其形态和轮廓与瑞一姬氏染色显示的包涵体形状、大小基本一致,但更为清晰;正常对照的粒细胞胞浆中未见包涵体。结论光学显微镜和电子显微镜进一步确认了家系患者包涵体的存在。免疫荧光技术显示患者和正常人之间NMMHC-IIA在中性粒细胞中的分布有差异;MYH9-RD患者中性粒细胞包涵体的主要构成成份是NMMHC-IIA;包涵体的形态结构复杂多样可能与本家系临床表型复杂多样有关,该家系对进一步揭示MYH9-RD的致病机制具有重要的意义。 展开更多
关键词 非肌球蛋白重9相关疾病(MYH9-RD) 中性粒细胞包涵体 非肌性肌球蛋白IIA(NMMHC-IIA)
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非肌性肌球蛋白重链9基因相关疾病2例报告并文献复习 被引量:3
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作者 郑湧智 李健 胡建达 《临床儿科杂志》 CAS CSCD 北大核心 2019年第12期893-897,共5页
目的探讨非肌性肌球蛋白重链9基因相关疾病(MYH9-RD)的基因突变及临床特征.方法回顾分析同一家系2例MYH9-RD患者的临床资料,并复习相关文献.结果先证者(男,1岁5个月)及其父亲(30岁)均有巨大血小板、血小板减少和粒细胞内包涵体,先证者... 目的探讨非肌性肌球蛋白重链9基因相关疾病(MYH9-RD)的基因突变及临床特征.方法回顾分析同一家系2例MYH9-RD患者的临床资料,并复习相关文献.结果先证者(男,1岁5个月)及其父亲(30岁)均有巨大血小板、血小板减少和粒细胞内包涵体,先证者之父还存在神经性耳聋,均曾误诊免疫性血小板减少症,接受激素治疗无效.先证者及其父亲存在MYH9基因c.4270G>A杂合突变,导致氨基酸改变p.D1424N,为错义突变.国内已报道48个家系MYH9-RD,38个有基因测序结果,其中c.4270G>A(p.D1424N)为国内报道最多的MYH9基因突变类型.结论结合细胞形态学及基因检测有利于MYH9-RD的早期诊断,c.4270G>A(p.D1424N)为国内最为常见的MYH9基因突变类型. 展开更多
关键词 非肌性肌球蛋白重9基因相关疾病 巨大血小板减少症 基因突变
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非肌性肌球蛋白重链9基因相关疾病的研究进展 被引量:1
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作者 陈印宜 文小红 +1 位作者 张应爱 张淑芳 《广西医学》 CAS 2019年第14期1813-1816,共4页
非肌性肌球蛋白重链9(MYH9)基因相关疾病是由MYH9基因突变所引起的一组罕见的常染色体显性遗传性疾病,临床表现以巨大血小板、血小板减少和中性粒细胞包涵体为主,部分患者还可出现感音神经性耳聋、先天性白内障或间质性肾炎等症状。近... 非肌性肌球蛋白重链9(MYH9)基因相关疾病是由MYH9基因突变所引起的一组罕见的常染色体显性遗传性疾病,临床表现以巨大血小板、血小板减少和中性粒细胞包涵体为主,部分患者还可出现感音神经性耳聋、先天性白内障或间质性肾炎等症状。近年来国内外关于MYH9基因相关疾病的报道较多,但其发病机制、基因型与表型之间的关系等尚未明确。本文就MYH9基因相关疾病的研究进展进行综述。 展开更多
关键词 非肌性肌球蛋白重9基因相关疾病 常染色体显性遗传 非肌性肌球蛋白重ⅡA 基因型与表型 综述
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儿童散发性MYH9相关疾病1例报告 被引量:1
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作者 张丽宁 孙蕾 +4 位作者 匡新宇 王平 康郁林 吴滢 黄文彦 《临床儿科杂志》 CAS CSCD 北大核心 2022年第8期623-626,共4页
非肌性肌球蛋白重链9相关疾病(MYH9-RD)是遗传性血小板减少症的病因之一,常伴有肾脏损害、感觉神经性耳聋、白内障等血液系统以外的表现。患儿,女,13岁,血小板减少12年余,血尿、蛋白尿4年余。外周血涂片镜检存在血小板数量下降(10~30)&#... 非肌性肌球蛋白重链9相关疾病(MYH9-RD)是遗传性血小板减少症的病因之一,常伴有肾脏损害、感觉神经性耳聋、白内障等血液系统以外的表现。患儿,女,13岁,血小板减少12年余,血尿、蛋白尿4年余。外周血涂片镜检存在血小板数量下降(10~30)×10^(9)/L,血小板体积增大。尿常规示蛋白+++,红细胞(15~17)/HP,24小时尿蛋白定量4.34 g[相当于76 mg/(kg·d)]。纯音阈测定提示高频听力受损,眼科检查提示存在早发性白内障。基因检测证实患儿存在MYH9基因c.2104C>T(p.R702C)位点杂合突变,为已见文献报道的致病性变异,家系验证患儿父母、哥哥均为野生型。明确诊断为MYH9-RD。先后予缬沙坦、环孢素治疗,患儿肾脏损害持续性进展,并进展至CKD2期。白内障及听力损害亦进行性加重。对于难治性血小板减少的患儿需警惕MYH9-RD可能,基因检测有助于早期明确断。 展开更多
关键词 非肌性肌球蛋白重9相关疾病 MYH 9基因 儿童
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院前急救“全流程”管理思考与建议 被引量:1
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作者 秦兆亮 《江苏卫生事业管理》 2023年第12期1759-1763,共5页
研究分析当前国家、省、市院前急救管理规章制度和管理现状,剖析相关问题,提出构建以“呼叫第一时刻”为起点、以“救护车返站待命”为终点的院前急救“全流程”管理模式和理念,完善“全流程”管理方法和举措,以时间为轴、以疾病为链,... 研究分析当前国家、省、市院前急救管理规章制度和管理现状,剖析相关问题,提出构建以“呼叫第一时刻”为起点、以“救护车返站待命”为终点的院前急救“全流程”管理模式和理念,完善“全流程”管理方法和举措,以时间为轴、以疾病为链,持续优化院前急救服务流程,保证院前急救医疗服务的及时性、规范性、有效性。 展开更多
关键词 院前急救 全流程 时间轴 疾病链
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新生儿非肌性肌球蛋白重链9基因相关疾病1例
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作者 米乐园 尹姣姣 +4 位作者 张钏 胡晓燕 赵盼 董雪梅 刘小晖 《中华新生儿科杂志(中英文)》 CAS CSCD 2023年第8期503-504,共2页
本文报道1例非肌性肌球蛋白重链9基因相关疾病新生儿,患儿母亲孕前即发现血小板减少,治疗效果不佳,产前血小板波动于(9~71)×10^(9)/L,检测血小板功能正常,患儿生后血小板64×10^(9)/L,全外显子组测序发现母婴均存在MYH9基因第4... 本文报道1例非肌性肌球蛋白重链9基因相关疾病新生儿,患儿母亲孕前即发现血小板减少,治疗效果不佳,产前血小板波动于(9~71)×10^(9)/L,检测血小板功能正常,患儿生后血小板64×10^(9)/L,全外显子组测序发现母婴均存在MYH9基因第41号外显子c.5797C>T(p.Arg1933Ter)杂合变异,诊断明确,患儿无出血表现,未予治疗。 展开更多
关键词 非肌性肌球蛋白重9基因相关疾病 MYH9基因 血小板减少症
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Chronic disease and the link to physical activity 被引量:9
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作者 J. Larry Durstine Benjamin Gordon +1 位作者 ZhengzhenWang Xijuan Luo 《Journal of Sport and Health Science》 SCIE 2013年第1期3-11,共9页
Chronic diseases have become a focal point of public health worldwide with estimates of trillions of dollars in annual health care cost and causing more than 36 million deaths a year. Lifestyle factors such as physica... Chronic diseases have become a focal point of public health worldwide with estimates of trillions of dollars in annual health care cost and causing more than 36 million deaths a year. Lifestyle factors such as physical inactivity are heavily correlated with the development of many chronic diseases. New strategies for primary and secondary disease prevention are desperately needed to aid in blunting the negative economic and social impact of these diseases. Physical activity (PA) and exercise are now considered principal interventions for use in primary and secondary prevention of chronic diseases. Currently, more emphasis in primary prevention of disease is necessary to reduce disease risk in youth and adults; however with chronic disease prevalence so high, similar emphasis is also necessary for secondary prevention in those children and adults already inflicted with chronic diseases. Conditions such as cardiovascular disease, type 2 diabetes, obesity, and cancer are drastically improved when PA and exercise are part of a medical management plan. In addition, the national PA guidelines in conjunction with PA promotion tools like Exercise is MedicineTM are needed to promote increased PA and exercise levels worldwide. 展开更多
关键词 Chronic disease Physical activity Primary prevention Secondary prevention
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太原市小店区2008年餐饮业餐饮具消毒状况调查分析 被引量:3
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作者 刘龙云 孙亚雄 《实用医技杂志》 2009年第1期24-25,共2页
餐饮具的消毒是防止食源性疾病传播,阻断食源性疾病传播链的有效手段,是餐饮业卫生监督执法工作中需强化监督与指导的一个重要环节。为了进一步掌握我区餐饮单位餐饮具消毒状况,指导今后的卫生监督工作,2008年我们对全区620户次餐... 餐饮具的消毒是防止食源性疾病传播,阻断食源性疾病传播链的有效手段,是餐饮业卫生监督执法工作中需强化监督与指导的一个重要环节。为了进一步掌握我区餐饮单位餐饮具消毒状况,指导今后的卫生监督工作,2008年我们对全区620户次餐饮单位及学校食堂进行了10989份餐饮具消毒情况抽样检测调查,现将调查结果分析报告如下。 展开更多
关键词 餐饮具消毒 餐饮业 状况调查 太原市 卫生监督执法工作 疾病传播 卫生监督工作 餐饮单位
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Identification of AOSC-binding proteins in neurons
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作者 刘明 聂琴 +1 位作者 辛现良 耿美玉 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2008年第4期394-399,共6页
Acidic oligosaccharide sugar chain (AOSC), a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer's Disease (AD) drug c... Acidic oligosaccharide sugar chain (AOSC), a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer's Disease (AD) drug candidate. The identification of AOSC-binding protein(s) in neurons is very important for understanding its action mechanism. To determine the binding protein(s) of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 (AP-2) and amyloid precursor protein (APP). These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities. 展开更多
关键词 AOSC Alzheimer's disease marine oligosaccharide binding protein(s)
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Independent and combined effects of environmental factors and miR-126, miR-143, and miR-145 on the risk of coronary heart disease 被引量:10
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作者 Da-Cen LIN Jia-Bing LIN +6 位作者 Zhou CHEN Rong CHEN Chun-Yu WAN Shao-Wei LIN Qi-Shuang RUAN Huang-Yuan LI Si-Ying WU 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2017年第11期688-695,共8页
Objective To evaluate the effects of environmental factors and microRNAs (miRNAs) (miR-126, miR-143, and miR-145) on the risk of coronary heart disease (CHD). Methods A frequency-matched case-control study (450... Objective To evaluate the effects of environmental factors and microRNAs (miRNAs) (miR-126, miR-143, and miR-145) on the risk of coronary heart disease (CHD). Methods A frequency-matched case-control study (450 patients, 450 controls) was conducted from April 2014 to December 2016 in Fuzhou City, China. Environmental factors were investigated using a self-administered questionnaire, and the expression levels of miR-126, rniR-143, and miR-145 were determined by quantitative real-time Polymerase Chain Reaction (PCR) in pe- ripheral blood mononuclear cells. Unconditional logistic regression models were used for statistical evaluation. Results Alcohol consumption, high-salt diets, high-intensity work, and lack of physical activity were significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. MiR-126, miR-143, and miR-145 were highly expressed in the CHD group compared with the control group. After adjustment for other environmental factors, unconditional logistic regression results revealed that miR-126, miR-143, and depression were the independent risk factors of CHD, and light diet was the independent protective factor of CHD. Conclusions Our data suggest that a family history of CHD, anxiety, and alcohol consumption was significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. Furthermore, miR-126 and miR-143 in combination with several risk factors, could play a joint role in the development of CHD. Therefore, it is necessary to manage patients with CHD in all directions and multiple level. 展开更多
关键词 Case-control study Coronary heart disease Environmental factors Gene-environment interaction MiR- 126 MiR- 143 MiR- 145
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Molecular Detection, Culture and Isolation of Mycoplasma Pneumoniae From Reproductive Tract of STD Patients 被引量:1
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作者 王继东 赵季文 +5 位作者 李琴 徐萃瑜 谢平 华咏 汪圣强 肖琛月 《Chinese Journal of Sexually Transmitted Infections》 2002年第3期41-45,共5页
Objective: To confirm whether Mycoplasma pneumoniae (MP) are present in reproductive tract of STD patients inChina. Methods: Application of nested PCR (nPCR) and DNAsequencing to test samples of urethral/vaginal swabs... Objective: To confirm whether Mycoplasma pneumoniae (MP) are present in reproductive tract of STD patients inChina. Methods: Application of nested PCR (nPCR) and DNAsequencing to test samples of urethral/vaginal swabs withMP culture confirmation of several nPCR positive patients. Results: 74 of 786 STD patients were positive for MP bynPCR, with a rate of 9.4%. of the 484 male patients, 10.5%were positive, and among the 302 female patients, 7.6%were positive. There was no significant difference betweenthem (P<0.05). of 12 cases of MP positive samples by nPCR,4 cases were first generation culture-positive, and one ofthem passed to the next generation successfully. DNAsequencing was performed on the nPCR product of oneswab sample and one MP culture isolation. The determinedsequence was identical to the typical MP strain. Conclusion: In China, MP are present in reproductivetract of both male and female STD patients. 展开更多
关键词 MYCOPLASMA PNEUMONIAE PCR DNA sequence Determination
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The Role of NK Cell in T Cell Recruitment in Murine Liver Infected with Adenovirus 被引量:1
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作者 游上游 艾洪武 +1 位作者 黄巍 张楚瑜 《Journal of Microbiology and Immunology》 2003年第1期6-10,共5页
To study the role of natural killer (NK) cells in T cell recruitment in murine liver infected with virus, mice were intravenously injected daily with anti-NK1.1 + antibody to deplete NK cells. Lymphocytes in the liver... To study the role of natural killer (NK) cells in T cell recruitment in murine liver infected with virus, mice were intravenously injected daily with anti-NK1.1 + antibody to deplete NK cells. Lymphocytes in the liver tissue of mice infected with type 5 adenovirus depleted in the E1 and E3 regions were assessed by fluorometric activated cell sorting (FACS). Expression of chemokine IP-10 and its receptor CXCR3 mRNA in the liver, hepatic lymphocytes and spleen tissue were examined by reverse transcription polymerase chain reaction (RT-PCR). Serum alanine aminotransferase (ALT) was measured as an indicator of liver injury. It was found that infection of adenovirus and anti-Fas monoclonal antibody (mAb) into mice caused liver injury and high expression of interferon-γ inducible protein-10 (IP-10) mRNA in the liver. Anti-NK1.1 + mAb, which was intraperitoneally injected into the mice infected with adenovirus, suppresses T cell recruitment and expression of IP-10 mRNA in the liver. Slighter liver injury was also observed. After virus infection, expression of CXCR3 mRNA in spleen and liver tissue was observed at different time. The results suggested that T cell recruitment was initiated by NK cell dependent chemokine IP-10, which induced activated T cells priming in the spleen to the liver of the mouse. NK cells played a key role in T cell recruitment in the liver of mouse infected with adenovirus. 展开更多
关键词 Type 5 adenovirus NK cell CHEMOKINE Liver injury
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An Audit of Constraints and Opportunities in Kenya's Livestock Export Value Chain
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作者 Patrick Irungu Peter Ithondeka +3 位作者 Esther Wafula Sabenzia Wekesa Hezron Wesonga Thomas Manga 《Journal of Agricultural Science and Technology(B)》 2014年第2期102-120,共19页
For a long time Kenya has desired to access lucrative export markets for her livestock products. Although this desire matches the growing global demand for livestock products and increasing interest in livestock produ... For a long time Kenya has desired to access lucrative export markets for her livestock products. Although this desire matches the growing global demand for livestock products and increasing interest in livestock products from Kenya by livestock deficient countries, Kenya has not been able to meet the expressed demand. The reasons for this failure have not been sufficiently documented. This study used the value chain approach to assess and document the factors that limit Kenya's export trade in livestock. Questionnaire interviews were undertaken with livestock producers, traders, processors and importers. Producers cited livestock diseases, poor roads, drought, livestock theft and insecurity as the main constraints to livestock supply. Livestock traders mentioned high cost and delays in obtaining movement permits, rent-seeking and disturbance by government officials along the stock routes as some of the constraints. Processors on the other hand mentioned Kenya's failure to meet the international sanitary requirements. The only importer interviewed indicated that trade restrictions, Kenya's inability to control livestock diseases and distance were the main constraints to increased livestock trade with Kenya. Stakeholders in Kenya's livestock export value chain should address these and other constraints in order to revitalize this vital sector. 展开更多
关键词 Livestock export trade value chain analysis Kenya.
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Vidas CHL Assay in the Detection of Urogenital Chlamydia Trachomatis Infection
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作者 黄澍杰 程文海 +4 位作者 吴志周 柯建良 黄东辉 谢礼豪 谭仲楷 《Chinese Journal of Sexually Transmitted Infections》 2002年第2期32-36,共5页
Objectives: To evaluate the Vidas Chlamydia (CHL) assayfor detecting C.Trachomatis with swabs and first catch urine(FCU) specimens from STD patients and high riskpopulations. Methods: A total of 383 pahents were teste... Objectives: To evaluate the Vidas Chlamydia (CHL) assayfor detecting C.Trachomatis with swabs and first catch urine(FCU) specimens from STD patients and high riskpopulations. Methods: A total of 383 pahents were tested with tissueculture (TC), Vidas CHL and polymerase chain reaction (PCR)for C.trachomatis on male and female swabs, with Vidas CHLtesting male FCU specimens. CHL positive and equivocalresults were confirmed with a blocking assay (CHB). Truepositive were defined as either TC positive, or TC negtive butCHL and PCR positive. The performance of TC, CHL andPCR were evaluated according to this expanded goldstandard. Results: Compared with the expanded gold standard, 54 ofthe 232 male specimens were true positive results. For maleswabs, TC, CHL and PCR had sensitivities of 90.7%, 96.3%and 94.4%, and specificities of 100%, 98.3% and 97.2%,respectively. Differences were not statistically significant. Formale FCU specimens, CHL sensitivity and specificity were83.3% and 98.3%; there was little difference between theseresults and that of matched swabs. Compared with theexpanded gold standard, 28 of the 151 female swabs were truepositive; TC, CHL and PCR had sensitivities of 82.1%, 100%and 96.4%, and specificities of 100%, 98.4% and 97.6%,respectively. The difference was also not significant. Conclusions: Vidas CHL assay is very scnsitive and specificfor C.trachomatis detection with swab specimens of male andfemale STD patients. For male FCU specimens, the assay alsohad high sensitivity and specificity. CHB may not be needed inthe routine detection or Chlamydia infections. Populationswith higher incidence of C.trachomatis infection. 展开更多
关键词 Chlamydia trachomatis Vidas Chlamydia UROGENITAL SYSTEM ASSAY
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