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小麦黑胚病菌及其分离方法比较 被引量:3
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作者 康业斌 郭仲儒 +1 位作者 王江燕 陈卓 《种子》 CSCD 北大核心 1999年第6期19-20,共2页
河南省小麦黑胚菌主要是交链孢菌[包括细交链孢菌(Alternariatennis)与极细交链孢菌(A.tenuissima)]和麦类根腐德氏霉菌(Drechslerasorokimiana)。检验小麦黑胚病菌以组织分离法最为准确,以清水培养法较为简捷。
关键词 小麦 黑胚病 病原图 分离方法
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Analysis of the Protein Electrophoresis Patterns of Curvularia Lunata in Maize and Immunological Detection of the Pathogenic Culture
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作者 宁红 Gao Rong +2 位作者 QIN Zhen Wang Lihuan LIU Shigui 《High Technology Letters》 EI CAS 2002年第3期11-15,共5页
Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis(SDS PAGE) patterns of their soluble proteins. Difference... Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis(SDS PAGE) patterns of their soluble proteins. Differences exist significantly among different species and the same species. There is a special protein band for Curvularia at relative mobility value 0.177 (Rf 0.177) and a special protein band for C. lunata at Rf 0.225. Two kinds of primary antibodies to C. lunata are used to indirect ELISA and Avidin Biotin peroxidase Complex (ABC) immunoassay to evaluate their detecting sensitivity and specificity. The sensitivity of ABC immuno assay is higher than indirect ELISA. APAbs to C. lunata is special to C. lunata. 展开更多
关键词 MAIZE Curvularia lunata SDS PAGE immunological detection
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