不同病原学检查方法对蠕形螨检查效果的探讨

目的:探讨与分析不同病原学检查方法对蠕形螨的检查效果,寻求操作简便、检出率高、质量可靠,又能适用于医院门诊患者及流行病学调查的一种蠕形螨检查方法。方法:应用挤压刮取涂片法、透明胶纸粘贴法、刮脂法、眼睫毛检查等4种方法对同一人群进行蠕形螨检查。结果:4种方法检查蠕形螨阳性率分别为38.88%、26.57%、7.75%和18.47%,提示挤压刮取涂片法检出率高于其他3种检查法,差异有统计学意义(χ2=257.11,P<0.01),4种检查方法的检出率均显示女生高于男生,差异有统计学意义(χ2=33.52、10.36、4.79和11.00,P<0.01或P<0.05)。结论:挤压刮取涂片法检出率高于透明胶纸粘贴法和刮脂法及眼睫毛检查法。

肠道寄生虫粪便的病原学检查方法

在实验动物病原检测中,寄生虫检测是其中重要的分支,也是体现实验动物质量不可或缺的一个方面。寄生虫侵入宿主可在宿主体内产生多种有害作用包括夺取营养,机械性损伤,继发感染,毒性作用等,严重影响实验动物健康并降低实验动物的质量。许多寄生虫生活史中虫卵、卵囊、或幼虫随宿主的粪便排出体外,这些都可以通过粪便寄生虫检查,确定是否感染寄生虫以及感染的种类和程度。病原学检查是诊断寄生虫成虫及虫卵的重要的实验技术,采集粪便做粪便检查又是诊断实验动物消化道有无寄生虫感染比较常用的手段。实验动物粪检的病原学方法有多种。本文列举并介绍了一些目前比较常用的粪便寄生虫定性和定量的检查方法,旨在提高粪便寄生虫病原学检查的技术水平。

Primary duodenal neoplasms:A retrospective clinico-pathological analysis

AIM: To analyze the clinico-pathological spectrum of primary duodenal neoplasms. METHODS: A total of 55 primary duodenal neoplasms reported in the last 10 years after excluding ampullary and periampullary tumors were included in the study. Clinical details were noted and routine hematoxylin and eosin stained paraffin sections were studied for histological subtyping of the tumors. RESULTS: On histopathological examination primary duodenal neoplasms were categorized as: epithelial tumor in 27 cases (49.0%) including 10 cases of adenoma, 15 cases of adenocarcinoma, and 2 cases of Brunner gland adenoma; mesenchymal tumor in 9 cases (16.3%) consisting of 4 cases of gastrointestinal stromal tumor, 4 cases of smooth muscle tumor and I case of neurofibroma; lymphoproliferative tumor in 12 cases (21.8%), and neuroendocrine tumor in 7 cases (12.7%). CONCLUSION: Although non-ampullary/periampullary duodenal adenocarcinomas are rare, they constitute the largest group. Histopathological examination of primary duodenal tumors is important for correct histological subtyping.