OBJECTIVE: To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial ...OBJECTIVE: To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI). METHODS: Fifteen dogs were used, and 0.3 ml (1 x 10(9) pfu) of an adenovirus (Adex1SR LacZ) was injected into the heart by 3 methods. The dogs were killed 5 days following injection, and gene expressions in heart and liver were evaluated by histochemical analysis. RESULTS: The results showed that (1) the CAP method was relatively less damaging and induced sparse LacZ expression in the myocardium, and the gene expression was also found in both vessels within the myocardium and liver; (2) gene transfer by DMI resulted in intense LacZ expression around the injection accompanied by a local inflammatory response; (3) LacZ expression elicited by ICI was detected in either the inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium. CONCLUSION: Three catheter-mediated methods of gene transfer into the heart may be used and a reasonable approach should be chosen according to purpose.展开更多
To investigate the feasibility and features of 3 catheter mediated approaches o f gene transfer into heart, including direct myocardial injection (DMI), coron ary artery perfusion (CAP), and intrapericardial cavity...To investigate the feasibility and features of 3 catheter mediated approaches o f gene transfer into heart, including direct myocardial injection (DMI), coron ary artery perfusion (CAP), and intrapericardial cavity injection (ICI) Methods Fifteen dogs were used, and 0 3?ml (1×10 9 pfu) of an adenovirus (Adex1SR La cZ) was injected into the heart by 3 methods The dogs were killed 5 days foll owing injection, and gene expressions in heart and liver were evaluated by histochemical analysis Results The results showed that ① the CAP method was relatively less damaging and indu ced sparse LacZ expression in the myocardium, and the gene expression was also f ound in both vessels within the myocardium and liver; ② gene transfer by DMI res ulted in intense LacZ expression around the injection accompanied by a local inf lammatory response; ③ LacZ expression elicited by ICI was detected in either t he inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium Conclusion Three catheter mediated methods of gene transfer into the heart may be used a nd a reasonable approach should be chosen according to purpose展开更多
文摘OBJECTIVE: To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI). METHODS: Fifteen dogs were used, and 0.3 ml (1 x 10(9) pfu) of an adenovirus (Adex1SR LacZ) was injected into the heart by 3 methods. The dogs were killed 5 days following injection, and gene expressions in heart and liver were evaluated by histochemical analysis. RESULTS: The results showed that (1) the CAP method was relatively less damaging and induced sparse LacZ expression in the myocardium, and the gene expression was also found in both vessels within the myocardium and liver; (2) gene transfer by DMI resulted in intense LacZ expression around the injection accompanied by a local inflammatory response; (3) LacZ expression elicited by ICI was detected in either the inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium. CONCLUSION: Three catheter-mediated methods of gene transfer into the heart may be used and a reasonable approach should be chosen according to purpose.
文摘To investigate the feasibility and features of 3 catheter mediated approaches o f gene transfer into heart, including direct myocardial injection (DMI), coron ary artery perfusion (CAP), and intrapericardial cavity injection (ICI) Methods Fifteen dogs were used, and 0 3?ml (1×10 9 pfu) of an adenovirus (Adex1SR La cZ) was injected into the heart by 3 methods The dogs were killed 5 days foll owing injection, and gene expressions in heart and liver were evaluated by histochemical analysis Results The results showed that ① the CAP method was relatively less damaging and indu ced sparse LacZ expression in the myocardium, and the gene expression was also f ound in both vessels within the myocardium and liver; ② gene transfer by DMI res ulted in intense LacZ expression around the injection accompanied by a local inf lammatory response; ③ LacZ expression elicited by ICI was detected in either t he inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium Conclusion Three catheter mediated methods of gene transfer into the heart may be used a nd a reasonable approach should be chosen according to purpose
