为培育高滴度的鹅细小病毒(GPV)细胞适应病毒株,本研究将原代鹅胚成纤维细胞(GEF)进行连续传代培养,并传至31代次,传代的GEF具有典型的成纤维细胞形态,培养48 h后形成单层细胞。选取不同代次的GEF接种GPV YN分离株,并在GEF中连续传代至...为培育高滴度的鹅细小病毒(GPV)细胞适应病毒株,本研究将原代鹅胚成纤维细胞(GEF)进行连续传代培养,并传至31代次,传代的GEF具有典型的成纤维细胞形态,培养48 h后形成单层细胞。选取不同代次的GEF接种GPV YN分离株,并在GEF中连续传代至F20代,病毒滴度由102.5TCID50/0.1 m L达到106.16TCID50/0.1 m L,表明GPV已适应GEF,并在感染GEF后的72 h产生明显细胞病变。对细胞适应毒F20和亲本病毒F0代进行基因序列比对结果显示,F20与F0存在133个核苷酸差异位点,主要集中在5'和3'非编码区。氨基酸序列共出现22个变异位点,主要集中在VP3蛋白中。这些变异与病毒对细胞培养的适应过程有关,其机理有待进一步研究。展开更多
Herpes simplex virus type 1 （HSV-1）, a neurotropic member of the alphaherpes virus family, is among the most prevalent and successful human pathogens. HSV-1 can cause serious diseases at every stage of life includin...Herpes simplex virus type 1 （HSV-1）, a neurotropic member of the alphaherpes virus family, is among the most prevalent and successful human pathogens. HSV-1 can cause serious diseases at every stage of life including fatal disseminated disease in newborns, cold sores, eye disease, and fatal encephalitis in adults. HSV-1 infection can trigger rapid immune responses, and efficient inhibition and clearance of HSV-1 infection rely on both the innate and adaptive immune responses of the host. Multiple strategies have been used to restrict host innate immune responses by HSV-1 to facilitate its infection in host cells. The adaptive immunity of the host plays an important role in inhibiting HSV-1 infections. The activation and regulation of T cells are the important aspects of the adaptive immunity. They play a crucial role in host-mediated immunity and are important for clearing HSV-1. In this review, we examine the findings on T cell immune responses during HSV-1 infection, which hold promise in the design of new vaccine can- didates for HSV-I.展开更多
文摘为培育高滴度的鹅细小病毒(GPV)细胞适应病毒株,本研究将原代鹅胚成纤维细胞(GEF)进行连续传代培养,并传至31代次,传代的GEF具有典型的成纤维细胞形态,培养48 h后形成单层细胞。选取不同代次的GEF接种GPV YN分离株,并在GEF中连续传代至F20代,病毒滴度由102.5TCID50/0.1 m L达到106.16TCID50/0.1 m L,表明GPV已适应GEF,并在感染GEF后的72 h产生明显细胞病变。对细胞适应毒F20和亲本病毒F0代进行基因序列比对结果显示,F20与F0存在133个核苷酸差异位点,主要集中在5'和3'非编码区。氨基酸序列共出现22个变异位点,主要集中在VP3蛋白中。这些变异与病毒对细胞培养的适应过程有关,其机理有待进一步研究。
基金supported by the Wuhan Institute of Virology (WIV) “One-Three-Five” Strategic Programs,China
文摘Herpes simplex virus type 1 （HSV-1）, a neurotropic member of the alphaherpes virus family, is among the most prevalent and successful human pathogens. HSV-1 can cause serious diseases at every stage of life including fatal disseminated disease in newborns, cold sores, eye disease, and fatal encephalitis in adults. HSV-1 infection can trigger rapid immune responses, and efficient inhibition and clearance of HSV-1 infection rely on both the innate and adaptive immune responses of the host. Multiple strategies have been used to restrict host innate immune responses by HSV-1 to facilitate its infection in host cells. The adaptive immunity of the host plays an important role in inhibiting HSV-1 infections. The activation and regulation of T cells are the important aspects of the adaptive immunity. They play a crucial role in host-mediated immunity and are important for clearing HSV-1. In this review, we examine the findings on T cell immune responses during HSV-1 infection, which hold promise in the design of new vaccine can- didates for HSV-I.
