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传染性法氏囊病毒VP2蛋白的分子生物学研究进展 被引量:8
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作者 万勇 谢金文 余为一 《现代生物医学进展》 CAS 2006年第2期77-80,共4页
本文综述了IBDV的保护性抗原蛋白VP2与病毒形态、毒力变异、抗原变异间的关系及在诱导宿主细胞凋亡中的作用。同时也探讨了其在新型疫苗开发中的应用等方面的研究进展。
关键词 IBDV VP2蛋白 病毒构造 毒力变异 抗原变异 细胞凋亡 新型疫苗研究
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HIV-1 CRF01-AE、CRF07/08-BC和B′构造病毒株体外适应性比较
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作者 张麒 蒋岩 《疾病监测》 CAS 2010年第6期439-442,共4页
目的构建NL4-3env-EGFP/DsRed2前病毒,观察中国主要流行的HIV-1 CRF01-AE?CRF07/08-BC和B′亚型构造病毒株体外适应性。方法 5份HIV-1B′,CRF07/08-BC,CRF01-AE亚型感染的干血斑样本和1份Zambia C亚型PBMC DNA样本,经单基因组扩增技术获... 目的构建NL4-3env-EGFP/DsRed2前病毒,观察中国主要流行的HIV-1 CRF01-AE?CRF07/08-BC和B′亚型构造病毒株体外适应性。方法 5份HIV-1B′,CRF07/08-BC,CRF01-AE亚型感染的干血斑样本和1份Zambia C亚型PBMC DNA样本,经单基因组扩增技术获得env全长基因,克隆、亚克隆、转染等步骤构建14个NL4-3env-EGFP/DsRed2前病毒,按顺序进行配对竞争实验,流式细胞仪定量检测被感染细胞的数量。结果 CRF01-AE亚型前病毒适应性最强,适应性值W>1,其次为B′,Zambia C,CRF07-BC/CRF08-BC,P<0.05。结论可利用NL4-3env-EGFP/DsRed2构造前病毒,通过竞争实验比较流行病毒株的体外适应性,推测env基因对适应性的影响。 展开更多
关键词 HIV-1构造病毒 适应性 ENV基因
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An Overlay Network for Monitoring Activities of Computer Viruses
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作者 Li Ying Cao Yiqun +3 位作者 Qiu Ben Jiao Jian Shan Xiuming Ren Yong 《Engineering Sciences》 EI 2008年第1期52-58,共7页
To accurately track computer viruses,an overlay network that monitors the activities of viruses is constructed.Identifying and locating nodes infected by virus on network is achieved by a naming system in which a node... To accurately track computer viruses,an overlay network that monitors the activities of viruses is constructed.Identifying and locating nodes infected by virus on network is achieved by a naming system in which a node in the network is mapped to a unique serial number of the hard-drive.By carefully monitoring and recording sensitive communication between local system and remote nodes on the network,and suspicious operations on files that originate from remote nodes and entered via some form of file transfer,activities of viruses in both local and network level are recorded and ready for future analysis.These data can also be used in analysis of the mechanism of a computer virus as well as its spreading mode and pattern. 展开更多
关键词 overlay network VIRUS OBSERVATION DNS
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Crystal Structure of Chlorinate (N,N-1,3-Dibenzyl-benzimidazole) Hydrate
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作者 建方方 王焕香 +1 位作者 肖海连 刘光烨 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2003年第6期669-672,共4页
The structure of the title compound consisting of N,N-1,3-dibenzyl-benzimidazole ion, Cl- anion and hydrate was determined by X-ray diffraction method. It crystallizes in the triclinic system, space group P with a = 9... The structure of the title compound consisting of N,N-1,3-dibenzyl-benzimidazole ion, Cl- anion and hydrate was determined by X-ray diffraction method. It crystallizes in the triclinic system, space group P with a = 9.320(2), b = 9.850(2), c = 11.510(2) ? = 68.75(3), = 80.57(3), = 70.20(3)? C21H19ClN2H2O, Mr = 352.85, V = 925.6(3) 3, Z = 2, Dc = 1.266 g/cm3, F(000) = 372, = 0.217 mm-1, R = 0.0452 and wR = 0.1372. The total reflections were 3462 and 3244 (Rint = 0.0206) were independent, of which 1666 were observed with I > 2s(I). The planes of benzyl group are nearly perpendicular to each other. The water molecules with Cl-anions and C(8) atom are involved in hydrogen bonds and interactions, and - stacking interaction exists obviously between the adjacent phenyl and imidazole rings. The title complex forms a three-dimensional network throughout the structure through these hydrogen bonds and interactions. 展开更多
关键词 N N-1 3-dibenzylbenzimidazole crystal structure hydrogen bonds conformation
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