Subculture Helicoverpa armigera nuclear polyhedrosis virus （HaNPV） is an entomopathogenic virus isolated from cadaver of Helicoverpa armigera larvae, and is successfully produced in Spodoptera litura larvae as an al...Subculture Helicoverpa armigera nuclear polyhedrosis virus （HaNPV） is an entomopathogenic virus isolated from cadaver of Helicoverpa armigera larvae, and is successfully produced in Spodoptera litura larvae as an alternate host. These viral agents have been used to control the population of three species insect pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella, which were infested in cabbages plantation. The concentration of virus of 4 × 10^7 polyhedra/mL was sprayed in cabbage leaves 24 h before infested of larva and every four days after. The research used randomized design which consisted of one factor （three species of second instar larvae, S. litura, C. pavonana, P. xyllostella） and eight replications. The result showed that the three species of larvae were sensitive against subculture HaNPV infection. The mortality of each larval species （S. litura, C. pavonana and P. xyllostella） was 100%, 97.5% and 98.7%, respectively. Subculture HaNPV can be utilized to control the population of three species of insect pests in cabbage plantation.展开更多
OBJECTIVE: To investigate the compositions of Th1/Th2/Th3 cells in chronic hepatitis B virus (HBV)-infected individuals by determining the expression of interleukin-4 (IL-4), inetrferon-gamma (IFN-gamma), and transfor...OBJECTIVE: To investigate the compositions of Th1/Th2/Th3 cells in chronic hepatitis B virus (HBV)-infected individuals by determining the expression of interleukin-4 (IL-4), inetrferon-gamma (IFN-gamma), and transform growth factor-beta (TGF-beta) in single CD4(+) T cells isolated from peripheral blood mononuclear cells (PBMCs) and the role of polarized Th cell populations in chronic HBV-infection was discussed. METHODS: PBMCs from chronically infected HBV individuals were isolated, stimulated by PMA/Ionomycin/Monensin, and IL-4, IFN-gamma and TGF-beta production by CD4(+) T cells was determined by using fluorescence activated cell sorter (FACS) analysis. RESULTS: The percentage of IFN-gamma-producing T cells, IL-4-producing T cells and TGF-beta-producing T cells ranged from 2.3% - 18.6%, 1.1% - 8.7% and 0.7% - 7.1% respectively in CD4(+) T cells from non-infected individuals. Most of CD4(+) T cells from PBMCs in chronically infected HBV individuals were Th0 cells. The proportion of Th1 cells increased significantly with hepatic inflammatory activity, and in the active period of chronic hepatitis B infection were higher than those in the non-active period (P 0.05), but were higher than that from controls (P展开更多
文摘Subculture Helicoverpa armigera nuclear polyhedrosis virus （HaNPV） is an entomopathogenic virus isolated from cadaver of Helicoverpa armigera larvae, and is successfully produced in Spodoptera litura larvae as an alternate host. These viral agents have been used to control the population of three species insect pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella, which were infested in cabbages plantation. The concentration of virus of 4 × 10^7 polyhedra/mL was sprayed in cabbage leaves 24 h before infested of larva and every four days after. The research used randomized design which consisted of one factor （three species of second instar larvae, S. litura, C. pavonana, P. xyllostella） and eight replications. The result showed that the three species of larvae were sensitive against subculture HaNPV infection. The mortality of each larval species （S. litura, C. pavonana and P. xyllostella） was 100%, 97.5% and 98.7%, respectively. Subculture HaNPV can be utilized to control the population of three species of insect pests in cabbage plantation.
文摘OBJECTIVE: To investigate the compositions of Th1/Th2/Th3 cells in chronic hepatitis B virus (HBV)-infected individuals by determining the expression of interleukin-4 (IL-4), inetrferon-gamma (IFN-gamma), and transform growth factor-beta (TGF-beta) in single CD4(+) T cells isolated from peripheral blood mononuclear cells (PBMCs) and the role of polarized Th cell populations in chronic HBV-infection was discussed. METHODS: PBMCs from chronically infected HBV individuals were isolated, stimulated by PMA/Ionomycin/Monensin, and IL-4, IFN-gamma and TGF-beta production by CD4(+) T cells was determined by using fluorescence activated cell sorter (FACS) analysis. RESULTS: The percentage of IFN-gamma-producing T cells, IL-4-producing T cells and TGF-beta-producing T cells ranged from 2.3% - 18.6%, 1.1% - 8.7% and 0.7% - 7.1% respectively in CD4(+) T cells from non-infected individuals. Most of CD4(+) T cells from PBMCs in chronically infected HBV individuals were Th0 cells. The proportion of Th1 cells increased significantly with hepatic inflammatory activity, and in the active period of chronic hepatitis B infection were higher than those in the non-active period (P 0.05), but were higher than that from controls (P
