Genetic Modification of Baculovirus Expression Vectors

As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high level expression of heterologous proteins in both insect and mammalian cells. These modifications include utilization of different promoters and signal peptides, deletion or replacement of viral genes for increasing protein secretion, integration of polycistronic expression cassette for producing protein complexes, and baculovirus pseudotyping, promoter accommodation or surface display for enhancing mammalian cell targeting gene delivery. This review summarizes the development and the current state of art of the baculovirus expression system. Further development of baculovirus expression systems will make them even more feasible and accessible for advanced applications.

在降低rAAV基因药物免疫反应中减少载体剂量并保持高效表达和药效的实施策略

如何降低免疫反应是重组腺相关病毒(rAAV)基因药物面临的重大挑战。研究表明减少载体使用剂量能够降低rAAV基因药物的免疫反应,但是减少载体使用剂量同时也会降低靶基因的表达量,甚至可能达不到治疗效果。本文重点阐述如何在减少载体使用剂量的同时,保持基因表达强度和药效。尤其侧重讨论采取给药途径优化、病毒载体改造、启动子选取在增强基因治疗效果中的应用。