【目的】探讨外耳道乳头状瘤（PEAC）的病因机制,分析外耳道乳头状瘤与人乳头状瘤病毒（HPV）感染组织病理学基础。【方法】选取2011年1月至2016年12月本院收集的130例PEAC手术切除标本,采用HE染色和原位杂交技术检测低危型病毒（HPV6/1...【目的】探讨外耳道乳头状瘤（PEAC）的病因机制,分析外耳道乳头状瘤与人乳头状瘤病毒（HPV）感染组织病理学基础。【方法】选取2011年1月至2016年12月本院收集的130例PEAC手术切除标本,采用HE染色和原位杂交技术检测低危型病毒（HPV6/11）DNA感染和高危型病毒（HPV16/18）DNA感染情况。【结果】PEAC伴挖空细胞低危型HPV6/11DNA感染率（79.52%）显著高于PEAC不伴挖空细胞（P〈0.05）,而PEAC伴挖空细胞HPV16/18 DNA感染率（17.54%）高于PEAC不伴挖空细胞（0）,但差异不具有统计学意义（P〉0.05）;男性在HPV6/11DNA感染率（80.21%）显著高于女性（47.06%）（P〈0.05）,而男性和女性在HPV16/18 DNA感染率（21.87% vs 14.71%）比较,差异不具有统计学意义（P〉0.05）;HE染色结果显示,鳞状上皮呈乳头状增生,表皮轻度角化或重度角化,表层有角化不全细胞散在,中心结缔组织有淋巴细胞浸润,表皮浅层或中层细胞可见大量挖空细胞,真皮乳头部毛细血管增生扩张;原位杂交结果显示,HPV6/11阳性呈现细胞核阳性,而HPV16/18阳性主要呈现细胞质阳性。【结论】PEAC的致病机制可能由HPV病毒感染所致。展开更多
[Objective] This study aimed to investigate the histopathological changes in chickens artificially infected with infectious laryngotracheitis（ILT）. [Method] Through ar- tificial injection of allantoic liquid contain...[Objective] This study aimed to investigate the histopathological changes in chickens artificially infected with infectious laryngotracheitis（ILT）. [Method] Through ar- tificial injection of allantoic liquid containing ILTV into chickens to induce infectious laryngotracheitis, the clinical symptoms in infected chickens were observed; tissues of trachea, lungs, heart, liver, kidney and spleen were collected from dead chickens, and prepared into paraffin sections, followed by histopathological observation under a microscope. [Results] This disease occured in the inoculated chickens 3d later, with a morbidity rate of 95%. The main symptoms were dyspnea, asthma and coughing up of bloody exudate; yellowish-white pseudomembrane was observed on dissected larynx trachea; swelling, haemorrhage, and further erosion were observed on the in- fected tracheal mucosa, resulting in death of chickens, with the mortality rate of 25%; histopathological observation showed that parenchymal organs exhibited exuda- tive inflammation; swelling, degeneration, necrosis and shedding of epithelial cells were observed; disturbance of blood circulation occured.[Conclusion] This study pro- vides reference bases for the treatment of ILT.展开更多
目的:研究胃远端部分切除术后患者泌酸功能及其与黏膜病理改变、胆汁反流和幽门螺杆菌(Hp)感染的相关性。方法:采用24 h pH监测仪测定51例残胃患者空腹胃内pH值,根据24 h胃内平均pH值分为低泌酸组(pH≥3)和正常泌酸组(pH<3)。胃镜观...目的:研究胃远端部分切除术后患者泌酸功能及其与黏膜病理改变、胆汁反流和幽门螺杆菌(Hp)感染的相关性。方法:采用24 h pH监测仪测定51例残胃患者空腹胃内pH值,根据24 h胃内平均pH值分为低泌酸组(pH≥3)和正常泌酸组(pH<3)。胃镜观察残胃黏膜以及Hp检测。结果:低泌酸组患者35例,其中29例黏膜呈重度萎缩;正常泌酸组16例,其中4例呈重度黏膜炎症(P=0.006)。胆汁反流率在低泌酸组和正常泌酸组分别为37.4%和18.7%(P=0.014)。两组间Hp感染率无明显差异。62.7%的患者胃酸分泌减少,但却接受抑酸药物治疗。结论:24 h动态胃pH监测可有效评价残胃患者胃黏膜泌酸功能。胆汁反流和残胃炎程度与黏膜泌酸功能有明显相关性。约1/3残胃患者保持正常胃酸分泌功能,临床上有大量患者接受了不必要的抑酸治疗。展开更多
AIM： To investigate the effect of the serum of patients with chronic hepatitis B （CHB） on apoptosis of renal tubular epithelial cells in vitro and to study the role of hepatitis B virus （HBV） and transforming gro...AIM： To investigate the effect of the serum of patients with chronic hepatitis B （CHB） on apoptosis of renal tubular epithelial cells in vitro and to study the role of hepatitis B virus （HBV） and transforming growth factor-β1 （TGF-β1） in the pathogenesis of hepatitis B virus associated glomerulonephritis （HBV-GN）. METHODS： The levels of serum TGF-β1 were measured by specific enzyme linked immunosorbent assay （ELISA） and HBV DNA was tested by polymerase chain reaction （PCR） in 44 patients with CHB ,and 20 healthy persons as the control. The normal human kidney proximal tubular cell （HK-2） was cultured together with the sera of healthy persons, CHB patients with HBV-DNA negative（20 cases） and HBV-DNA positive （24 cases） for up to 72 h. Apoptosis and Fas expression of the HK-2 were detected by flow cytometer. RESULTS： The apoptosis rate and Fas expression of HK-2 cells were significantly higher in HBV DNA positive serum group 19.01±5.85% and 17.58±8.35%, HBV DNA negative serum group 8.12±2.80% and 6.96 ± 2.76% than those in control group 4.25±0.65% and 2.33 ± 1.09%, respectively （P 〈 0.01）. The apoptosis rate and Fas expression of HK-2 in HBV DNA positive serum group was significantly higher than those in HBV DNA negative serum （P 〈 0.01）. Apoptosis rate of HK-2 cells in HBV DNA positive serum group was positively correlated with the level of HBV-DNA （r = 0.657）. The level of serum TGF-β1 in CHB group was 163.05 ± 91.35 μg/L, signifi- cantly higher as compared with 81.40 ± 40.75 μg/L in the control group （P 〈 0.01）.CONCLUSION： The serum of patients with chronic hepatitis B promotes apoptotic damage in human renal tubular cells by triggering a pathway of Fas up-regulation. HBV and TGF-β1 may play important roles in the mechanism of hepatitis B virus associated glomerulonephritis.展开更多
文摘【目的】探讨外耳道乳头状瘤（PEAC）的病因机制,分析外耳道乳头状瘤与人乳头状瘤病毒（HPV）感染组织病理学基础。【方法】选取2011年1月至2016年12月本院收集的130例PEAC手术切除标本,采用HE染色和原位杂交技术检测低危型病毒（HPV6/11）DNA感染和高危型病毒（HPV16/18）DNA感染情况。【结果】PEAC伴挖空细胞低危型HPV6/11DNA感染率（79.52%）显著高于PEAC不伴挖空细胞（P〈0.05）,而PEAC伴挖空细胞HPV16/18 DNA感染率（17.54%）高于PEAC不伴挖空细胞（0）,但差异不具有统计学意义（P〉0.05）;男性在HPV6/11DNA感染率（80.21%）显著高于女性（47.06%）（P〈0.05）,而男性和女性在HPV16/18 DNA感染率（21.87% vs 14.71%）比较,差异不具有统计学意义（P〉0.05）;HE染色结果显示,鳞状上皮呈乳头状增生,表皮轻度角化或重度角化,表层有角化不全细胞散在,中心结缔组织有淋巴细胞浸润,表皮浅层或中层细胞可见大量挖空细胞,真皮乳头部毛细血管增生扩张;原位杂交结果显示,HPV6/11阳性呈现细胞核阳性,而HPV16/18阳性主要呈现细胞质阳性。【结论】PEAC的致病机制可能由HPV病毒感染所致。
基金Supported by the Special Project of Department of Science and Technology of Hebei Province(08820412D)the Special Project of Qinhuangdao Municipal Bureau of Science and Technology[Qinkeji(2003)30-35]+1 种基金the Special Project of Shijiazhuang Municipal Bureau of Science and Technology(07150193A)the Scientific Research Innovation Team Project of Hebei Normal University of Science and Technology(TD201201)~~
文摘[Objective] This study aimed to investigate the histopathological changes in chickens artificially infected with infectious laryngotracheitis（ILT）. [Method] Through ar- tificial injection of allantoic liquid containing ILTV into chickens to induce infectious laryngotracheitis, the clinical symptoms in infected chickens were observed; tissues of trachea, lungs, heart, liver, kidney and spleen were collected from dead chickens, and prepared into paraffin sections, followed by histopathological observation under a microscope. [Results] This disease occured in the inoculated chickens 3d later, with a morbidity rate of 95%. The main symptoms were dyspnea, asthma and coughing up of bloody exudate; yellowish-white pseudomembrane was observed on dissected larynx trachea; swelling, haemorrhage, and further erosion were observed on the in- fected tracheal mucosa, resulting in death of chickens, with the mortality rate of 25%; histopathological observation showed that parenchymal organs exhibited exuda- tive inflammation; swelling, degeneration, necrosis and shedding of epithelial cells were observed; disturbance of blood circulation occured.[Conclusion] This study pro- vides reference bases for the treatment of ILT.
文摘目的:研究胃远端部分切除术后患者泌酸功能及其与黏膜病理改变、胆汁反流和幽门螺杆菌(Hp)感染的相关性。方法:采用24 h pH监测仪测定51例残胃患者空腹胃内pH值,根据24 h胃内平均pH值分为低泌酸组(pH≥3)和正常泌酸组(pH<3)。胃镜观察残胃黏膜以及Hp检测。结果:低泌酸组患者35例,其中29例黏膜呈重度萎缩;正常泌酸组16例,其中4例呈重度黏膜炎症(P=0.006)。胆汁反流率在低泌酸组和正常泌酸组分别为37.4%和18.7%(P=0.014)。两组间Hp感染率无明显差异。62.7%的患者胃酸分泌减少,但却接受抑酸药物治疗。结论:24 h动态胃pH监测可有效评价残胃患者胃黏膜泌酸功能。胆汁反流和残胃炎程度与黏膜泌酸功能有明显相关性。约1/3残胃患者保持正常胃酸分泌功能,临床上有大量患者接受了不必要的抑酸治疗。
基金Supported by the Applied Basic Research Programs of Science and Technology Commission of Sichuan Province, No. 01SY051-29
文摘AIM： To investigate the effect of the serum of patients with chronic hepatitis B （CHB） on apoptosis of renal tubular epithelial cells in vitro and to study the role of hepatitis B virus （HBV） and transforming growth factor-β1 （TGF-β1） in the pathogenesis of hepatitis B virus associated glomerulonephritis （HBV-GN）. METHODS： The levels of serum TGF-β1 were measured by specific enzyme linked immunosorbent assay （ELISA） and HBV DNA was tested by polymerase chain reaction （PCR） in 44 patients with CHB ,and 20 healthy persons as the control. The normal human kidney proximal tubular cell （HK-2） was cultured together with the sera of healthy persons, CHB patients with HBV-DNA negative（20 cases） and HBV-DNA positive （24 cases） for up to 72 h. Apoptosis and Fas expression of the HK-2 were detected by flow cytometer. RESULTS： The apoptosis rate and Fas expression of HK-2 cells were significantly higher in HBV DNA positive serum group 19.01±5.85% and 17.58±8.35%, HBV DNA negative serum group 8.12±2.80% and 6.96 ± 2.76% than those in control group 4.25±0.65% and 2.33 ± 1.09%, respectively （P 〈 0.01）. The apoptosis rate and Fas expression of HK-2 in HBV DNA positive serum group was significantly higher than those in HBV DNA negative serum （P 〈 0.01）. Apoptosis rate of HK-2 cells in HBV DNA positive serum group was positively correlated with the level of HBV-DNA （r = 0.657）. The level of serum TGF-β1 in CHB group was 163.05 ± 91.35 μg/L, signifi- cantly higher as compared with 81.40 ± 40.75 μg/L in the control group （P 〈 0.01）.CONCLUSION： The serum of patients with chronic hepatitis B promotes apoptotic damage in human renal tubular cells by triggering a pathway of Fas up-regulation. HBV and TGF-β1 may play important roles in the mechanism of hepatitis B virus associated glomerulonephritis.
