Subculture Helicoverpa armigera nuclear polyhedrosis virus （HaNPV） is an entomopathogenic virus isolated from cadaver of Helicoverpa armigera larvae, and is successfully produced in Spodoptera litura larvae as an al...Subculture Helicoverpa armigera nuclear polyhedrosis virus （HaNPV） is an entomopathogenic virus isolated from cadaver of Helicoverpa armigera larvae, and is successfully produced in Spodoptera litura larvae as an alternate host. These viral agents have been used to control the population of three species insect pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella, which were infested in cabbages plantation. The concentration of virus of 4 × 10^7 polyhedra/mL was sprayed in cabbage leaves 24 h before infested of larva and every four days after. The research used randomized design which consisted of one factor （three species of second instar larvae, S. litura, C. pavonana, P. xyllostella） and eight replications. The result showed that the three species of larvae were sensitive against subculture HaNPV infection. The mortality of each larval species （S. litura, C. pavonana and P. xyllostella） was 100%, 97.5% and 98.7%, respectively. Subculture HaNPV can be utilized to control the population of three species of insect pests in cabbage plantation.展开更多
The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France...The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France and Italy. A laboratory insect colony was started from insects collected in a French resistant orchard. It was named RGV. Various virus isolates were identified as active against this resistant insect colony. Field tests were carried out in 2007 to test if the two virus isolates CpGV-I12 and NPP-R1 were effective in the field. Although these virus isolates were not able to reduce insect caused fruit damages, they significantly reduced the overwintering insect populations. NPP-R1 was subjected to eight passages on RGV larvae (NPP-R1.8) that improved its biological activity on RGV larvae. 2008 field trials were set up to test this improved virus strain, compared to CpGV-I12 and Madex plus active on RGV. These tests confirmed the ability to control both in susceptible and resistant insect populations.展开更多
文摘Subculture Helicoverpa armigera nuclear polyhedrosis virus （HaNPV） is an entomopathogenic virus isolated from cadaver of Helicoverpa armigera larvae, and is successfully produced in Spodoptera litura larvae as an alternate host. These viral agents have been used to control the population of three species insect pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella, which were infested in cabbages plantation. The concentration of virus of 4 × 10^7 polyhedra/mL was sprayed in cabbage leaves 24 h before infested of larva and every four days after. The research used randomized design which consisted of one factor （three species of second instar larvae, S. litura, C. pavonana, P. xyllostella） and eight replications. The result showed that the three species of larvae were sensitive against subculture HaNPV infection. The mortality of each larval species （S. litura, C. pavonana and P. xyllostella） was 100%, 97.5% and 98.7%, respectively. Subculture HaNPV can be utilized to control the population of three species of insect pests in cabbage plantation.
基金supported by the French Research Agency (ANR-06-RIB-003-02) and by NPP
文摘The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France and Italy. A laboratory insect colony was started from insects collected in a French resistant orchard. It was named RGV. Various virus isolates were identified as active against this resistant insect colony. Field tests were carried out in 2007 to test if the two virus isolates CpGV-I12 and NPP-R1 were effective in the field. Although these virus isolates were not able to reduce insect caused fruit damages, they significantly reduced the overwintering insect populations. NPP-R1 was subjected to eight passages on RGV larvae (NPP-R1.8) that improved its biological activity on RGV larvae. 2008 field trials were set up to test this improved virus strain, compared to CpGV-I12 and Madex plus active on RGV. These tests confirmed the ability to control both in susceptible and resistant insect populations.
