痛经片中丹参、香附及白芍的薄层色谱鉴别

目的研究痛经片中丹参、香附及白芍的专属鉴别方法。方法采用薄层色谱鉴别方法。结果鉴别方法专属、灵敏、重现性好。结论可供痛经片中上述药材鉴别参考。

高效液相色谱法测定八味痛经片中木香烃内酯、去氢木香内酯和芍药苷

目的建立高效液相色谱法同时测定八味痛经片(牛膝、木香、当归、白芍等)中木香烃内酯、去氢木香内酯和芍药苷。方法色谱柱:Dikma Diamonsil C18柱,流动相:乙腈-0.085%磷酸溶液(15∶85),体积流量为1.0 mL/min,柱温:室温,检测波长:230 nm。结果木香烃内酯、去氢木香内酯和芍药苷进样量分别在0.094 6μg～0.946μg(r=0.999 5),0.095 20μg～0.952μg(r=0.999 9),0.105 3μg～1.053 0μg(r=0.999 3)范围内线性关系良好,平均加样回收率(n=9)分别为98.2%、98.1%、98.5%。结论本法简便,准确,重现性好,能排除其他成分的干扰能更好的控制该药质量。

HPLC法测定痛经片中阿魏酸的含量

采用高效液相色谱法，ＯＤＳ柱，以乙腈－０．０５ ８ ｍｏｌ／Ｌ ＫＨ２ＰＯ４溶液（用磷酸调ｐＨ＝２．５）（１：４）为流动相，测定痛经片中阿魏酸的含量。其方法回收率为９３．３％，ＲＳＤ为０．９％。

痛经片治疗功能性痛经115例

痛经为妇科常见病,多发病,临床以功能性痛经多见,简称“功痛”,现代研究表明:“功痛”是内分泌功能失调的表现,其实质是血中PG_(FZX)值升高所致。中医认为“不通则痛”是导致“功痛”发生的主要病机,现代医学对本病治疗缺少特效药物。痛经片对气滞血瘀、寒湿凝滞型痛经疗效显著,动物实验及临床观察证实,该药能显著降低血中PG_(FZX)水平,临床115例患者系统用药治愈率86.04％,总有效率93.86％。

痛经片的药理实验研究

'痛经片'系张文阁教授经过长期的临床实践总结出的治疗功能性痛经的有效方剂,为了进一步探讨'痛经片'的作用机理及其毒副作用,我们进行了以下实验。一、'痛经片'对小鼠痛阈的影响(一)实验材料1.动物:雌性昆明种小鼠30只,体重18～22克,由西安制药厂提供。2.药物:(1)痛经片。

止痛化症片治疗痛经的疗效观察

目的：观察止痛化症片治疗痛经的疗效。方法：回顾性分析我院2008年1月-2010年1月我院诊治的64例痛经患者，随机分为观察组（32例）和对照组（32例），观察组应用止痛化症片进行治疗，治疗组用消炎痛。结果：观察组总有效率为93．8％，优于对照组81．2％。结论：止痛化症片符合中医治疗痛经的理论，亦符合现代医药学理论，疗效显著，值得推广应用。

痛经灵片的制备与临床应用

痛经灵片为中药片剂,其组方原则主要是依据原发性痛经多为气滞血瘀和寒凝气滞血瘀而配制的。为观察痛经灵片的临床疗效,我们以妇科常用调经药当归浸膏片为对照组进行痛经灵的疗效观察,获得了比较满意的效果。现报告如下。痛经灵片的制备一、处方炒小茴香、元胡、土元、乌药等。二、制备方法取炒小茴香、元胡各1/3量,粉碎成80～100目细粉,备用。

靓丽淑女常遇痛经尴尬 非处方药轻松帮您解忧

治疗痛经的非处方药中艾附暖宫丸、痛经片偏于温经散寒,妇科得生丸、元胡止痛片偏于理气止痛。实证痛经,气滞或寒凝特征明显者,可以对证选用,一般可任意选用一种。虚者一般见经期或经净后疼痛,多为小腹隐隐作痛或腰背酸软疼痛,按之痛减,伴经色浅淡、面色苍白、精神倦怠、头晕等。

Interaction of δ-opioid Receptor with Membrane Transporters: Possible Mechanisms in Pain Suppression by Acupuncture

Objective： To investigate the possible mechanisms in acupuncture analgesia by interaction of δ-opioid receptor with neurotransmitter transport proteins or the Na^＋-K^＋ pump. Methods： Microinjection of respective heterologous cRNA into the Xenopus oocytes as a model system, and measurement of steady-state currents under two-electrode voltage clamp. Results： The co-expression of the 8-opioid receptor with GAT1, EAAC 1 or the sodium pump resulted in reducing activity of the respective transporter. Opioid receptor activation affected transporter activity in different ways： 1） GAT1 was further inhibited; 2） EAAC1 was stimulated; 3） Na^＋-K^＋ pump activity interfered with agonist sensitivity of DOR. Pump inhibition led to higher sensitivity for DPDPE. Conclusion： GABA transporter inhibition and glutamate transporter stimulation may counteract pain sensation by affecting the neurotransmitter concentration in the synaptic cleft and, therefore, may contribute synergistically to pain suppression by acupuncture. Sodium pump inhibition by endogenous ouabain may amplify these effects. These synergistic effects may be the molecular mechanism of inhibiting pain sense and/or acupuncture analgesia.

Experimental study on the effect of An-pressing and Rou-kneading Huantiao (GB 30) on certain brain nuclei of pleasure circuits in rats with chronic neuralgia

Objective:To explore the central neurobiological mechanisms of pleasure effect on rats with neuralgia treated by tuina manipulations of An-pressing and Rou-kneading Huantiao (GB 30).Methods:A total of 64 male Sprague-Dawley (SD) rats were used in this study.Eighteen rats were randomly selected as a normal group,and the other 46 rats were used to duplicate the chronic constriction injury (CCI) model.Ten rats failed in modeling and 36 rats succeeded.These 36 rats were then randomly divided into a model group and a tuina group,with 18 rats in each group.The rats in the normal group and the model group did not receive any interventions,while those in the tuina group received An-pressing and Rou-kneading Huantiao (GB 30),1 min for each time,once a day,3 weeks in total.Heating tests were evaluated to observe the change of pain-sensitivity score before intervention,1 week after intervention,2 weeks after intervention,and 3 weeks after intervention.After 1 week of intervention,2 weeks of intervention,and 3 weeks of intervention,6 rats were randomly selected from each group respectively for brain extraction.The change of Nissl's body and β-endorphin in the accumbens nucleus as well as amygdaloid nucleus of pleasure circuits,and pro-opiomelanocortin (POMC) in the arcuate nucleus were analyzed by methods of histochemistry and molecular biology.Results:After modeling,the pain-sensitivity scores of the tuina group and the model group were statistically different from the score of the normal group (both P<0.05).After An-pressing and Rou-kneading Huantiao (GB 30) for one week,the pain-sensitivity score of the tuina group had statistical difference compared with that of the model group (P<0.05).At each different time point:the amounts of Nissl's body in accumbens nucleus and amygdaloid nucleus of the tuina group were significantly more than those of the model group (all P<0.01).Besides,the numbers of β-endorphin immunoreactive cells in the accumbens nucleus and amygdaloid nucleus of the rats in the tuina group were significantly higher than those in the model group (all P<0.01),and so was the expression of POMC in arcuate nucleus (all P<0.01).Conclusion:An-pressing and Rou-kneading Huantiao (GB 30),where the sciatic nerve is ligated,can reduce pain-sensitivity score and increase pain tolerance value of rats with chronic neuralgia.It can increase the activity of neurons in accumbens nucleus and amygdaloid nucleus of pleasure circuits,which indicates that the analgesia effect of tuina therapy may correlate with pleasure effect,and also reveals a part of neurobiological mechanisms of neuralgia.

神经痛丸辅助治疗腰椎间盘突出症170例临床观察

目的观察神经痛丸辅助治疗腰椎间盘突出症(LDH)的临床疗效。方法将340例LDH患者采用随机数字表法分为治疗组和对照组,各170例。2组均给予常规治疗,对照组在常规治疗的基础上予万通筋骨片治疗,治疗组在常规治疗的基础上予神经痛丸治疗,2组均以1周为1个疗程,连续治疗2个疗程后比较2组的临床疗效及治疗前后的疼痛数字评价量表(NRS)评分。结果对照组总有效率为81.18%,治疗组为94.70%,2组比较差异有统计学意义(P<0.05);治疗后2组NRS评分均明显降低,与同组治疗前比较差异有统计学意义(P<0.01),且治疗组降低更明显,与对照组治疗后比较差异有统计学意义(P<0.05)。结论神经痛丸辅助治疗LDH临床疗效显著,可明显减轻患者疼痛,值得临床推广应用。