[Objective] The paper was to study the dynamic changes of forage nutrient substance fermentation in rumen, and a set of continuous culture system of artificial rumen was designed. [Method] With in vivo as control, the...[Objective] The paper was to study the dynamic changes of forage nutrient substance fermentation in rumen, and a set of continuous culture system of artificial rumen was designed. [Method] With in vivo as control, the simulating rumen fer- mentation effect in vitro culture system was evaluated. [Result] The simulation rumen fermentation test needed adaptive phase of 2-3 d, and the fermentation state was relatively stable within 3-9 d, with good effects. The test showed certain regularity variation with index value of rumen in vivo. [Conclusion] The continuous culture sys- tem of artificial rumen could be used as the ideal model to study the rumen fermen- tation in vivo.展开更多
AIM： To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation. METHODS： The expressions of cell adhesion molecules （including E-cadherin, N-cadherin, α-catenin, ...AIM： To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation. METHODS： The expressions of cell adhesion molecules （including E-cadherin, N-cadherin, α-catenin, β-catenin） and cadherin transcription factors including snail, slug and twist were determined by reverse transcriptasepolymerase chain reaction（RT-PCR）, immunoblotting and immunofluorescence in SV40-immortalized human gastric cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901. RESULTS： All cell lines expressed N-cadherin, but not E-cadherin. N-cadherin immunofluorescence was detected at cell membranous adherents junctions where co-localization with immunofluorescent staining of inner surface adhesion proteins α- and β-catenins was observed. The transformed Ges-1 and gastric cancer cell lines all expressed transcription factors （snail, slug and twist） which inhibited the expression of E-cadherin and triggered epithelial-mesenchymal transformation. CONCLUSION： Cadherin isoforms can change from E-cadherin to N-cadherin in transformed human gastric cancer cells, which is associated with intracellular events of stomach carcinogenesis and high expression of corresponding transcription factors.展开更多
文摘[Objective] The paper was to study the dynamic changes of forage nutrient substance fermentation in rumen, and a set of continuous culture system of artificial rumen was designed. [Method] With in vivo as control, the simulating rumen fer- mentation effect in vitro culture system was evaluated. [Result] The simulation rumen fermentation test needed adaptive phase of 2-3 d, and the fermentation state was relatively stable within 3-9 d, with good effects. The test showed certain regularity variation with index value of rumen in vivo. [Conclusion] The continuous culture sys- tem of artificial rumen could be used as the ideal model to study the rumen fermen- tation in vivo.
基金Supported by the National Natural Science Foundation of China,No.30370555 Supported by the National Natural Science Foundation of China,No.30270658+1 种基金National Major Basic Research Development Program No.G2000057002"211"project.
文摘AIM： To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation. METHODS： The expressions of cell adhesion molecules （including E-cadherin, N-cadherin, α-catenin, β-catenin） and cadherin transcription factors including snail, slug and twist were determined by reverse transcriptasepolymerase chain reaction（RT-PCR）, immunoblotting and immunofluorescence in SV40-immortalized human gastric cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901. RESULTS： All cell lines expressed N-cadherin, but not E-cadherin. N-cadherin immunofluorescence was detected at cell membranous adherents junctions where co-localization with immunofluorescent staining of inner surface adhesion proteins α- and β-catenins was observed. The transformed Ges-1 and gastric cancer cell lines all expressed transcription factors （snail, slug and twist） which inhibited the expression of E-cadherin and triggered epithelial-mesenchymal transformation. CONCLUSION： Cadherin isoforms can change from E-cadherin to N-cadherin in transformed human gastric cancer cells, which is associated with intracellular events of stomach carcinogenesis and high expression of corresponding transcription factors.
