期刊文献+
共找到6篇文章
< 1 >
每页显示 20 50 100
前列腺癌干细胞标志物研究进展 被引量:1
1
作者 王顺启 黄盛松 《中华男科学杂志》 CAS CSCD 2013年第12期1133-1137,共5页
前列腺癌是严重影响男性健康的恶性疾病,其发生、发展机理仍未完全明了。近年来前列腺癌干细胞的研究取得了多方面的进展,研究材料涉及人前列腺癌组织、前列腺癌细胞系,以及鼠前列腺癌模型。前列腺癌干细胞的研究为前列腺癌的发生和发... 前列腺癌是严重影响男性健康的恶性疾病,其发生、发展机理仍未完全明了。近年来前列腺癌干细胞的研究取得了多方面的进展,研究材料涉及人前列腺癌组织、前列腺癌细胞系,以及鼠前列腺癌模型。前列腺癌干细胞的研究为前列腺癌的发生和发展提出了一个全新的理念,对于前列腺癌的治疗具有积极意义。本文就近些年来在前列腺癌干细胞研究中应用于分离鉴定前列腺癌干细胞的标志物情况进行综述。 展开更多
关键词 前列腺 干细胞 癌干细胞标志物
下载PDF
不同生长环境中膀胱癌干细胞相关标志物CD44、CD133表达情况的初步研究 被引量:1
2
作者 程继文 蒙清贵 +1 位作者 唐勇 王植柔 《广西医学》 CAS 2010年第6期635-638,共4页
目的探讨在不同环境中观察膀胱癌细胞表达癌干细胞相关标志物CD44、CD133的变化情况,为进一步研究人体膀胱癌干细胞提供背景资料和前期数据。方法采用免疫组织化学与流式细胞术等技术,以膀胱癌5637细胞系为研究对象,通过建立体外培养、... 目的探讨在不同环境中观察膀胱癌细胞表达癌干细胞相关标志物CD44、CD133的变化情况,为进一步研究人体膀胱癌干细胞提供背景资料和前期数据。方法采用免疫组织化学与流式细胞术等技术,以膀胱癌5637细胞系为研究对象,通过建立体外培养、皮下移植瘤以及裸鼠淋巴结转移模型等模拟不同的生长微环境,观察CD44、CD133等癌干细胞相关标志物的表达情况。结果在体外培养环境中,膀胱癌5637的细胞膜蛋白CD44阳性表达,CD133则阴性表达;膀胱癌裸鼠移植瘤中CD44阳性表达,而CD133弱阳性表达。与体外培养体系比较,流式细胞术检测发现膀胱癌细胞5637中的CD44阳性表达水平无显著变化,而CD133阳性表达水平显著高于体外培养体系(P<0.01);在裸鼠淋巴结转移模型中,转移淋巴结均有CD44、CD133阳性表达。结论体内外环境的改变可以调控膀胱癌细胞中部分癌干细胞相关标志物的变化,也提示膀胱癌5637细胞系存在癌干细胞。 展开更多
关键词 膀胱 干细胞 癌干细胞标志物 CD44 CD133
下载PDF
膀胱癌细胞在不同生长环境中癌干细胞相关标志物CD44、CXCR4表达情况的初步研究 被引量:1
3
作者 程继文 蒙清贵 +1 位作者 唐勇 王植柔 《中国临床新医学》 2010年第10期923-926,共4页
目的观察癌干细胞相关标志物CD44、CXCR4在不同环境下膀胱癌细胞的变化情况,为人体膀胱癌干细胞的进一步研究提供前期数据与背景资料。方法采用免疫组织化学与流式细胞术等技术,对体外培养、皮下移植瘤以及裸鼠淋巴结转移模型等不同的... 目的观察癌干细胞相关标志物CD44、CXCR4在不同环境下膀胱癌细胞的变化情况,为人体膀胱癌干细胞的进一步研究提供前期数据与背景资料。方法采用免疫组织化学与流式细胞术等技术,对体外培养、皮下移植瘤以及裸鼠淋巴结转移模型等不同的生长微环境的膀胱癌5637细胞系癌干细胞相关标志物CD44、CXCR4的表达进行检测。结果在体外培养环境中,膀胱癌5637的细胞膜蛋白CD44呈阳性表达,而CXCR4呈弱阳性表达;膀胱癌裸鼠移植瘤中CD44、CXCR4均呈阳性表达。与体外培养体系比较,流式细胞术检测发现膀胱癌细胞中5637的CD44阳性表达水平无显著变化,而CXCR4阳性表达水平显著高于体外培养体系(P<0.01);在裸鼠淋巴结转移模型中,转移淋巴结均有CD44、CXCR4阳性表达。结论膀胱癌细胞中部分癌干细胞相关标志物的变化受体内外不同环境变化的调控,也提示膀胱癌5637细胞系存在有癌干细胞。 展开更多
关键词 膀胱 干细胞 癌干细胞标志物
下载PDF
卵巢癌干细胞标志物与化疗耐药研究进展
4
作者 陈百奇 韩世愈 《中国优生与遗传杂志》 2021年第4期586-589,共4页
卵巢癌是女性第五大最常见的死因,目前,治疗手段主要以手术切除肿瘤组织联合化疗为主,但后期患者往往出现复发及化疗耐药。研究结果发现,肿瘤干细胞是恶性肿瘤复发及对化疗药物耐药的根本所在,同时,提示肿瘤干细胞也可能是根治卵巢癌的... 卵巢癌是女性第五大最常见的死因,目前,治疗手段主要以手术切除肿瘤组织联合化疗为主,但后期患者往往出现复发及化疗耐药。研究结果发现,肿瘤干细胞是恶性肿瘤复发及对化疗药物耐药的根本所在,同时,提示肿瘤干细胞也可能是根治卵巢癌的潜在靶点,本文就卵巢癌干细胞标志物与化疗耐药研究进展进行综述。 展开更多
关键词 卵巢 卵巢癌干细胞标志物 化疗耐药
原文传递
Cancer stem cell markers CD133 and CD24 correlate with invasiveness and differentiation in colorectal adenocarcinoma 被引量:14
5
作者 Dongho Choi Hyo Won Lee +6 位作者 Kyung Yul Hur Jae Joon Kim Gyeong-Sin Park Si-Hyong Jang Young Soo Song Ki-Seok Jang Seung Sam Paik 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第18期2258-2264,共7页
AIM:To verify that CD markers are available for detecting cancer stem cell populations and to evaluate their clinical significance in colon cancer.METHODS:Immunohistochemistry for CD133,CD24 and CD44 was performed on ... AIM:To verify that CD markers are available for detecting cancer stem cell populations and to evaluate their clinical significance in colon cancer.METHODS:Immunohistochemistry for CD133,CD24 and CD44 was performed on the tissue microarray of 523 colorectal adenocarcinomas.Medical records were reviewed and clinicopathological analysis was performed.RESULTS:In colorectal adenocarcinoma,128 of 523 cases(24.5%) were positive and 395 cases(75.5%) were negative for CD133 expression.Two hundred and sixty-four of 523 cases(50.5%) were positive and 259 cases(49.5%) were negative for CD24 expression.Five hundred and two of 523 cases(96%) were negative and 21 cases(4%) were positive for CD44 expression.Upon clinicopathological analysis,CD133 expression was present more in male patients(P = 0.002) and in advanced T stage cancer(P = 0.024).Correlation between CD24 expression and clinicopathological factors was seen in the degree of differentiation(P = 0.006).Correlation between CD44 expression and clinicopathological factors was seen in the tumor size(P = 0.001).Survival was not significantly related to CD133,CD24 and CD44 expression.CONCLUSION:CD markers were related to invasiveness and differentiation of colorectal adenocarcinoma.However,CD expression was not closely related to survival. 展开更多
关键词 CD133 CD24 CD44 Colon cancer stemcells Colorectal adenocarcinoma
下载PDF
The expression and clinical significance of β-catenin and colorectal cancer stem cells marker EpCAM^(high)/CD44^+ in colorectal cancer
6
作者 Dan Liu Jinghua Sun +2 位作者 Jinming Zhu Huan Zhou Yang Zhang 《The Chinese-German Journal of Clinical Oncology》 CAS 2013年第12期581-585,共5页
Objective: The aim of the study was to explore the role of Wnt βcatenin signalling pathway in the maintenance, invasion and metastasis of colorectal cancer stem cells. Methods: Double immunohistochemical staining w... Objective: The aim of the study was to explore the role of Wnt βcatenin signalling pathway in the maintenance, invasion and metastasis of colorectal cancer stem cells. Methods: Double immunohistochemical staining was used to detect the expression of EpCAMhigh/CD44~ which is regarded as the marker of colorectal cancer stem cells in 80 cases of colorectal cancer and their corresponding liver metastases. The SP method of immunohistochemistry was used to detect the expression of the key protein βcatenin in the Wnt pathway in these tissue. The expression and correlation of ^-catenin and EpCAMh^gh/ CD44+ in colorectal cancer were analyzed and their role on the biological behavior of colorectal cancer was explored. Results: The abnormal expression of βcatenin was significantly higher in colorectal cancer than in the paraneoplastic normal intes- tinal mucosa [55% (44/80) vs 10% (2/20), P 〈 0.05]. The positive expression of EpCAMhigh/CD44+ was significantly higher in colorectal cancer than in the paraneoplastic normal intestinal mucosa [66.25% (53/80) vs 0% (0/20), P 〈 0.05]. In the 80 cases of colorectal cancer, the abnormal expression of ^-catenin has no correlation with gender (P = 0.079), age (P = 0.416) and the magnitude (P = 0.816) of the tumor (P 〉 0.05), but it was significantly correlated with degree of differentiation (P = 0.001), depth of invasion (P = 0.001), clinical stage (P = 0.000) and metastasis (P = 0.000). In the colorectal cancer, the expression of EpCAMhi^h/CD44~ cells has no correlation with gender (P = 0.934) and the magnitude (P = 0.160) of the tumor (P 〉 0.05), but was significantly correlated with age (P = 0.021), degree of differentiation (P = 0.013), depth of invasion (P = 0.000), clinical stage (P = 0.000) and metastasis (P = 0.000). In the corresponding liver metastases, we could also detecte EpCAMhih/CD44+ cells. In cases with abnormal expression of βcatenin, the positive expression rate of EpCAMhigh/CD44+ was significantly higher than those with normal expression of β-catenin (84.1% vs 44.4%), and the difference was statistically significant (P 〈 0.05). Conclusion: The abnormal activation of Wnt β-catenin signalling pathway may prompt the abnormal proliferation of the colorectal cancer stem cells, which leads to the recurrence and metastasis of the cancer. 展开更多
关键词 cancer stem cells double immunohistochemical staining βcatenin EpCAMhigh/CD44*
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部