AIM： TO investigate the significance of S phase kinase- associated protein 2 （Skp2） expression in human gastric carcinoma and the relation between expressions of Skp2, p27 and PTEN. METHODS： Immunohistochemical an...AIM： TO investigate the significance of S phase kinase- associated protein 2 （Skp2） expression in human gastric carcinoma and the relation between expressions of Skp2, p27 and PTEN. METHODS： Immunohistochemical analysis was performed on 138 gastric carcinoma specimens, their paired adjacent mucosa specimens, 102 paired lymphatic metastatic carcinoma tissue specimens, 30 dysplasia specimens, 30 intestinal metaplasia specimens, 10 chronic superficial gastritis specimens and 5 normal gastric mucosa specimens for Skp2 expression and on 138 gastric carcinoma specimens for p27 and PTEN expression. RESULTS： Skp2 labeling frequency was significantly higher in intestinal rnetaplasia （12.68±0.86） and adjacent rnucosa （19.324±1.22） than in normal gastric rnucosa （0.53±0.13） and chronic superficial gastritis （0.47±0.19） （P = 0.000）, in dysplasia （16.74±0.82）than in intestinal metaplasia （P = 0.000）; in gastric primary carcinoma （31.34±2.17） than in dysplasia and adjacent rnucosa （P = 0.000）, in metastasis gastric carcinoma in lymph nodes （39.76±2.00） than in primary gastric carcinoma （P = 0.037）, respectively. Skp2 labeling frequency was positively associated with differentiation degree （rho = 0.315, P = 0.000）, vessel invasion （rho = 0.303, P = 0.000） and lymph node metastasis （rho = 0.254, P = 0.000） of gastric cancer. Expression of Skp2 was negatively associated with p27 （rho = -0.451, P = 0.000） and PTEN （rho = -0.480, P = 0.000） expression in gastric carcinoma, p27 expression was positively associated with PTEN expression in gastric carcinoma （rho = 0.642, P = 0.000）.CONCLUSION： Skp2 overexpression may be involvedin carcinogenesis and progression of human gastric carcinoma in vivo, possibly via p27 proteolysis. PTEN may regulate the expression of p27 by negatively regulating Skp2 expression.展开更多
文摘AIM： TO investigate the significance of S phase kinase- associated protein 2 （Skp2） expression in human gastric carcinoma and the relation between expressions of Skp2, p27 and PTEN. METHODS： Immunohistochemical analysis was performed on 138 gastric carcinoma specimens, their paired adjacent mucosa specimens, 102 paired lymphatic metastatic carcinoma tissue specimens, 30 dysplasia specimens, 30 intestinal metaplasia specimens, 10 chronic superficial gastritis specimens and 5 normal gastric mucosa specimens for Skp2 expression and on 138 gastric carcinoma specimens for p27 and PTEN expression. RESULTS： Skp2 labeling frequency was significantly higher in intestinal rnetaplasia （12.68±0.86） and adjacent rnucosa （19.324±1.22） than in normal gastric rnucosa （0.53±0.13） and chronic superficial gastritis （0.47±0.19） （P = 0.000）, in dysplasia （16.74±0.82）than in intestinal metaplasia （P = 0.000）; in gastric primary carcinoma （31.34±2.17） than in dysplasia and adjacent rnucosa （P = 0.000）, in metastasis gastric carcinoma in lymph nodes （39.76±2.00） than in primary gastric carcinoma （P = 0.037）, respectively. Skp2 labeling frequency was positively associated with differentiation degree （rho = 0.315, P = 0.000）, vessel invasion （rho = 0.303, P = 0.000） and lymph node metastasis （rho = 0.254, P = 0.000） of gastric cancer. Expression of Skp2 was negatively associated with p27 （rho = -0.451, P = 0.000） and PTEN （rho = -0.480, P = 0.000） expression in gastric carcinoma, p27 expression was positively associated with PTEN expression in gastric carcinoma （rho = 0.642, P = 0.000）.CONCLUSION： Skp2 overexpression may be involvedin carcinogenesis and progression of human gastric carcinoma in vivo, possibly via p27 proteolysis. PTEN may regulate the expression of p27 by negatively regulating Skp2 expression.
