严重多发伤患者血清TNF-α,IL-6、IL-8的变化及意义

目的 探讨在严重创伤患者发病过程中肿瘤坏死因子TNF α,白介素IL 6、8的变化意义。方法 随机选择 4 0例多发伤患者 ,治疗中有 1 7例发生多脏器功能不全综合征 (MODS) ,其余 2 3例未发生MODS。在入院后各时段测定其血清肿瘤坏死因子TNF α,白介素IL 6、8水平 ,比较MODS组和非MODS组测定值的差别。结果 发现非MODS组患者TNF α,IL 6、8的表达在伤后早期即升高 ,2～ 3d后达高峰后开始下降 ,康复时恢复正常、而并发MODS者 ,以上值的早期上升幅度更明显、持续时间长、且出现高峰在伤后 7～ 1 0d。两组在伤后第 2天有明显差异 ,在第 4、7、1 0天有显著性差异。结论 TNF α,IL 6、8的促炎因子在全身性炎症反应综合征 (SIRS)中起着重要的作用 。

高血压患者急性心肌梗死后血清炎性因子的变化

目的探讨高血压患者在心肌梗死后炎性因子的变化及意义。方法急性心肌梗死患者87例根据高血压病史及入院当时血压分为血压正常组(43例)及高血压组(44例),另设健康对照组(25例)。采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)测定各组受试者入院即刻、第24h、第48h、第5天、第7天、第14天血清白介素1β(IL-1β)、白介素6(IL-6)、白介素8(IL-8)、肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)含量,健康对照组测1次上述各项指标。结果1)与对照组相比,心肌梗死患者的炎性因子浓度于发病2周内明显升高,呈先升后降的动态变化。2)炎性因子含量除高血压组的血清IL-6第5天(P=0.019)至第7天(P=0.005)的数值较血压正常组有明显升高外,其他数值两组无明显差异。结论炎症参与了急性心肌梗死全过程,在急性心肌梗死早期的炎性反应中,高血压对部分炎性因子起到了一定的促进作用。

THERAPEUTIC MECHANISM OF ELECTROACUPUNCTURE IN THE TREATMENT OF ULCERATIVE COLITIS IN THE RAT

Aim: To investigate the regulatory effects of electroacupuncture (EA) at "Zusanli" (ST 36) on proinflammatory cytokines and myeloperoxidase (MPO) activity in rats with ulcerative colitis (UC), and further elucidate the underlying mechanism of EA on UC. Methods: Thirty-two male SD rats were randomly and evenly divided into four groups: normal control group; UC control group; "Zusanli" (ST 36, EA) group; non-acupoint group. A solution containing ethanol and 2,4,6-trinitrobenzenesulfonate was instilled into the distal colon in the rat (at a dose of 100 mg/kg) to set up UC model. Non-anesthetized rats of EA group were stimulated at ST-36 by electroacupuncture once a day, while those of non-acupoint group were done at the site 0.5 cm beside ST-36. After 10 days’ treatment, all rats were sacrificed simultaneously. Colon mucosal inflammation and damage were assessed by measuring colon mass, morphologic damage score, colonic MPO activity, contents of serum interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-α). Morphologic damage score was examined under stereomicroscope. Colonic MPO was measured by spectrophotometric method. Serum TNF-α,IL-6 and IL-8 were determined by radioimmunoassay (RIA). Results: Both ratio of colonic mass/body mass (mC/mB) and activity of colonic MPO (μkat/g) in rats with UC were markedly increased (P<0.01 vs normal control group). Concentrations of serum TNF-α, IL-6 and IL-8 (ng/L) in rats of UC control group were significantly elevated (P<0.01 vs normal control group). In EA group, mC/mB and activity of MPO were decreased markedly compared with those of UC control group (P<0.05). Serum levels of TNF-α, IL-6 and IL-8 of ST-36 group were decreased by EA of ST-36 (P<0.01; P<0.05; P<0.05 vs UC control group). In comparison with UC control group, no significant changes were observed in the non-acupoint group. Furthermore, there were significant correlations among these parameters. Conclusion: In UC rats, serum TNF-α, IL-6 and IL-8 levels and MPO activity all increase significantly, indicating that these cytokines may cause inflammatory and immune response. EA stimulation induced downregulation on serum TNF-α, IL-6 and IL-8 levels and MPO activity may be responsible for its therapeutic effect in improving UC.

Electroacupuncture on serum interleukin level in rat models of cerebral ischemia-reperfusion injury

Objective： To observe the effect of electroacupuncture （EA） on serum interleukin （IL）-6, IL-8 and IL-10 in rat models of cerebral ischemia-reperfusion, and to discover the mechanism of EA in preventing and treating cerebral ischemia. 〈br〉 Methods：Male Sprague Dawley （SD） rats were randomized into a sham-operation （SO） group, a model control （MC） group, and an EA group, which were sub-grouped into a 6-hour group and a 24-hour group. In the SO group, rats only received vessel separation with filament placed inside without any treatment. In the MC and EA groups, the focal cerebral ischemia-reperfusion model was induced by using modified Longa method with intraluminal filament. The MC group didn’t receive any treatment;the EA group received EA at Baihui （GV 20） and Dazhui （GV 14） with sparse-dense wave for 30 min. The levels of serum IL-6, IL-8 and IL-10 were detected by using Elisa test. 〈br〉 Results： Six hours after ischemia-reperfusion injury, the levels of serum IL-6, IL-8 and IL-10 in the MC group were significantly higher than those in the SO group （P〈0.01, P〈0.05, P〈0.05）;the level of serum IL-8 in the EA group was significantly lower than that in the MC group （P〈0.05）, while there were no significant differences in comparing IL-6 and IL-10 between the EA group and the MC group. Twenty-four hours after ischemia-reperfusion injury, the levels of serum IL-6 and IL-8 in the EA group were significantly lower than those in the MC group （both P〈0.05）, while there were no significant differences in comparing the level of IL-10 among the three groups. 〈br〉 Conclusion：Early intervention by EA can regulate the levels of serum IL-6 and IL-8 in cerebral ischemic injury.

Clinical Study on Kidney-Nourishing and Governor Vessel-Regulating Acupuncture Therapy for Apoplexy Sequela

Objective： To observe the clinical efficacy of kidney-nourishing and governor vessel-regulating therapy for apoplexy sequela. Methods： Sixty subjects were equally randomized into observation group and control group, and respectively treated for 35 days. The scores of survival quality, interleukin-6（IL-6）, interleukin-8 （IL-8） and tumor necrosis factor（TNF） were measured, and the clinical efficacy were compared between two groups. Results and Conclusion： After treatment, all the observational items were improved, with better results in observation group than in control group. This therapy has better effects than regular method in the treatment of apoplexy sequela.