Partial cDNA sequence of rabbit BMP15 was cloned by RT-PCR from rabbit ovaries, showing a similarity of 83%-90% with the BMP15 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMP15...Partial cDNA sequence of rabbit BMP15 was cloned by RT-PCR from rabbit ovaries, showing a similarity of 83%-90% with the BMP15 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMP15 in rabbit cumulus-oocyte complexs during oocytes in vitro maturation （IVM） was measured by fluorescent quantitative RT-PCR method. BMP 15 was expressed at low levels in immature oocytes and increased to the highest level at 16h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly under IVM cultivation. The expression pattern of BMP 15 suggested that it might be important in cumulus expansion in rabbits.展开更多
[Objective] The aim was to discuss the histological characteristics of ovary development of Varicorhinus gerlachi.[Method] The annual variation of ovary and oocyte development of Varicorhinus gerlachi were observed an...[Objective] The aim was to discuss the histological characteristics of ovary development of Varicorhinus gerlachi.[Method] The annual variation of ovary and oocyte development of Varicorhinus gerlachi were observed and studied by means of histological method.[Result] Ovary development of Varicorhinus gerlachi was divided into 6 stages,and oocyte development had 6 phases,namely oocytes were in original differentiation state in phase Ⅰ,and yolk nucleus could be found obviously in phase Ⅱ.Vacuoles appeared in oocyte in phase Ⅲ,while oocytes differentiated obviously in phase Ⅳ,with the amalgamation of some yolk granules.Eggs formed and oocytes degenerated in phase V and Ⅵ.The development of ovary belonged to non-synchronous and multiple spawning type.In addition,Varicorhinus gerlachi began to spawn in April,and peak period was from May to July.[Conclusion] The study could provide technical parameters for the artificial propagation of Varicorhinus gerlachi.展开更多
[Objective] A study on separation process of lysozyme, ovotransferrin and ovalbumin from egg white. [Method] The proteins were separated by ammonium sul-fates and ion-exchange chromatography. Purity of the proteins wa...[Objective] A study on separation process of lysozyme, ovotransferrin and ovalbumin from egg white. [Method] The proteins were separated by ammonium sul-fates and ion-exchange chromatography. Purity of the proteins was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. [Result] The results showed that the proteins were electrophoresis-pure. The specific activity of lysozyme was increased from 144.13 to 2 235 U/mg, and purification factor was 15-fold. Lysozyme recovery rate was estimated to be 15.76%. Bacteriostasis rate of ovotransferrin was 48.84%. [Conclusion] The procedure for separating lysozyme, ovotransferrin and ovalbumin from egg white was simple, fast, low-cost and suitable for industrilization.展开更多
Monoclonal antibodies (McAb) were prepared by fusing sp2/0 myeoma cell with spleen cell of mice Balb/c which were immunized with 64 kDa rabbit oviductin (DPF-1). Supernatants of hybridoma cell were screened for produc...Monoclonal antibodies (McAb) were prepared by fusing sp2/0 myeoma cell with spleen cell of mice Balb/c which were immunized with 64 kDa rabbit oviductin (DPF-1). Supernatants of hybridoma cell were screened for producing anti-DPF1 McAbs with enzyme-linked immunosorbent assay (ELISA) and Western blotting. Positive hybrids were cloned by using the technique of limiting dilution. Two strains of hybridoma cell were obtained,named 3E8 and 5A7. Both McAbs were determined as I'm. Results of Western blotting showed that the two McAbs recognised the DPF-1 hand (64kDa) specifically. By using McAb-5A7 as probe, we found that DPF-1 did not occur in the tissues of spleen, uterus, ovary,small intestine, skeleton muscle, brain, liver,heart, lung and kidney, except for that of oviduct; some DPF-1 homologous molecules were also revealed itself in that of oviduct tissues of mouse and golden hamster Their apparent molecular weights were 32kDa,72kDa in mouse, and 49kDa in golden hamster. All these results indicated that DPF-1 is tissue specific not species specific. (To whon all corrcspondcnce should be addrcssed. Prcsent addrcss Shanghai Institute of Planned Parenthood Rcscarch. Shanghai 200032. PRC. )展开更多
A cathepsin B-like proteinase was purified from eggs of Antheraea pernyi by 5 steps of purification. The molecular mass of the proteinase are estimated to be 47 kDa by using SDS-polyacrylamide gel electro-phoresis. Th...A cathepsin B-like proteinase was purified from eggs of Antheraea pernyi by 5 steps of purification. The molecular mass of the proteinase are estimated to be 47 kDa by using SDS-polyacrylamide gel electro-phoresis. The activity of the purified proteinase were strongly inhibited by E-64 and Leupeptin. The optimum pH is 3.5 as determined by using the bovine hemo-globin as substrate. The activity and quantity of the proteinase during embryo development were studied. The results suggested that the proteolytic activities during embryo development at least partially came from this proteinase.展开更多
文摘Partial cDNA sequence of rabbit BMP15 was cloned by RT-PCR from rabbit ovaries, showing a similarity of 83%-90% with the BMP15 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMP15 in rabbit cumulus-oocyte complexs during oocytes in vitro maturation （IVM） was measured by fluorescent quantitative RT-PCR method. BMP 15 was expressed at low levels in immature oocytes and increased to the highest level at 16h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly under IVM cultivation. The expression pattern of BMP 15 suggested that it might be important in cumulus expansion in rabbits.
文摘[Objective] The aim was to discuss the histological characteristics of ovary development of Varicorhinus gerlachi.[Method] The annual variation of ovary and oocyte development of Varicorhinus gerlachi were observed and studied by means of histological method.[Result] Ovary development of Varicorhinus gerlachi was divided into 6 stages,and oocyte development had 6 phases,namely oocytes were in original differentiation state in phase Ⅰ,and yolk nucleus could be found obviously in phase Ⅱ.Vacuoles appeared in oocyte in phase Ⅲ,while oocytes differentiated obviously in phase Ⅳ,with the amalgamation of some yolk granules.Eggs formed and oocytes degenerated in phase V and Ⅵ.The development of ovary belonged to non-synchronous and multiple spawning type.In addition,Varicorhinus gerlachi began to spawn in April,and peak period was from May to July.[Conclusion] The study could provide technical parameters for the artificial propagation of Varicorhinus gerlachi.
基金Supported by Lishui Science and Technology Bureau Company-College Collaboration Program(20080410)~~
文摘[Objective] A study on separation process of lysozyme, ovotransferrin and ovalbumin from egg white. [Method] The proteins were separated by ammonium sul-fates and ion-exchange chromatography. Purity of the proteins was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. [Result] The results showed that the proteins were electrophoresis-pure. The specific activity of lysozyme was increased from 144.13 to 2 235 U/mg, and purification factor was 15-fold. Lysozyme recovery rate was estimated to be 15.76%. Bacteriostasis rate of ovotransferrin was 48.84%. [Conclusion] The procedure for separating lysozyme, ovotransferrin and ovalbumin from egg white was simple, fast, low-cost and suitable for industrilization.
文摘Monoclonal antibodies (McAb) were prepared by fusing sp2/0 myeoma cell with spleen cell of mice Balb/c which were immunized with 64 kDa rabbit oviductin (DPF-1). Supernatants of hybridoma cell were screened for producing anti-DPF1 McAbs with enzyme-linked immunosorbent assay (ELISA) and Western blotting. Positive hybrids were cloned by using the technique of limiting dilution. Two strains of hybridoma cell were obtained,named 3E8 and 5A7. Both McAbs were determined as I'm. Results of Western blotting showed that the two McAbs recognised the DPF-1 hand (64kDa) specifically. By using McAb-5A7 as probe, we found that DPF-1 did not occur in the tissues of spleen, uterus, ovary,small intestine, skeleton muscle, brain, liver,heart, lung and kidney, except for that of oviduct; some DPF-1 homologous molecules were also revealed itself in that of oviduct tissues of mouse and golden hamster Their apparent molecular weights were 32kDa,72kDa in mouse, and 49kDa in golden hamster. All these results indicated that DPF-1 is tissue specific not species specific. (To whon all corrcspondcnce should be addrcssed. Prcsent addrcss Shanghai Institute of Planned Parenthood Rcscarch. Shanghai 200032. PRC. )
文摘A cathepsin B-like proteinase was purified from eggs of Antheraea pernyi by 5 steps of purification. The molecular mass of the proteinase are estimated to be 47 kDa by using SDS-polyacrylamide gel electro-phoresis. The activity of the purified proteinase were strongly inhibited by E-64 and Leupeptin. The optimum pH is 3.5 as determined by using the bovine hemo-globin as substrate. The activity and quantity of the proteinase during embryo development were studied. The results suggested that the proteolytic activities during embryo development at least partially came from this proteinase.
