海藻酸钠复合涂膜对鲜切胡萝卜白变的影响

以鲜切胡萝卜为原料,以海藻酸钠、明胶、硬脂酸钠组成复合涂膜,在单因素试验的基础上,采用De-sign-Expert软件中的Box-Behnken中心组合设计,考察海藻酸钠复合涂膜不同浓度组成对鲜切胡萝卜白变的影响。结果表明:曲面回归方程拟合性好,抑制鲜切胡萝卜白变的适宜可食性膜组合为:海藻酸钠浓度10.28g/L、明胶浓度4.98g/L、硬脂酸钠浓度2.55g/L,在此涂膜组合条件下,鲜切胡萝卜的白度值为30.64。所建立的数学模型能较准确预测鲜切胡萝卜的白度值。

鲜切胭脂萝卜白变的影响因素分析

以涪陵特产胭脂萝卜为研究对象,通过品质指标间的相关性分析,研究贮藏期间品质变化规律,探索鲜切胭脂萝卜白变机理。结果表明:影响鲜切胭脂萝卜白变的关键因素有过氧化物酶、苯丙氨酸解氨酶、失重率、硬度和花青素。随着贮藏时间的增加,白度值上升,其中苯丙氨酸解氨酶、失重率和硬度与白度值相关性极显著,过氧化物酶、花青素与白度值相关性显著。为鲜切胭脂萝卜进入市场以及工业化生产提供理论依据。

“白变黑”诱导纳米TiO_(2)产生光热转换性能的综合实验设计

设计了黑色纳米二氧化钛(TiO_(2))制备及“白变黑”诱导产生光热转换性能的综合性实验。以锐钛矿和金红石结构的白色纳米TiO_(2)为原料,以硼氢化钠(NaBH)为还原剂,采用高温还原法制备了具有光热转换性能的黑色纳米TiO_(2),研究了还原剂浓度和反应时间对纳米TiO_(2)“白变黑”光吸收性质的影响,比较了两种结构纳米TiO_(2)“白变黑”诱导的光热转换性能。当NaBH与TiO_(2)的质量比为1∶3、反应温度和反应时间为350℃和120 min时,得到的黑色纳米TiO_(2)的光吸收性能最佳;在808 nm激光照射下,黑色纳米TiO_(2)显示出优异的光热转换性能,且在同等条件下,金红石结构黑色纳米TiO_(2)具有更好的光热转换性能。教学实践表明,纳米TiO_(2)“白”变“黑”实验不仅培养了学生在纳米材料制备、表征和数据分析方面的能力,而且对纳米材料的形貌-结构-性能之间的关系有了更深刻的理解。

视乳头白变误诊为神经萎缩的临床观察

视乳头白变虽是诊断视神经萎缩的主要体征之一,但决不是唯一的依据,还应从多方面加以综合评定.例如视乳头的位置、形态、境界、凹陷,色调及既往全身和眼病史等等都要一一注意到,特别是视功能的检查,其中屈光状况和视野检查尤为重要,否则容易造成误诊.我院曾遇几例记述如下.

手术服为何由白变绿

不少细心的人发现:医院手术室的医生们所穿的白大褂不知从什么时候开始变成了绿色

Physiological Character and Gene Mapping in a New Green-revertible Albino Mutant in Rice

A green-revertible albino mutant-Qiufeng M was found from the japonica rice （Oryza sativa L. ssp. japonica） Qiufeng in the field. The first three leaves of the mutant were albino with some green. The leaf color became pale green since the fourth leaf and the glume had the same phenomenon as the first three leaves. The measuring data of the pigment content confirmed the visually observed results. It truly had a remarkable changing process in the leaf color in Qiufeng M. Comparison of the main agronomic characters between Qiufeng and Qiufeng M indicated that the neck length and grain weight showed significant difference at the 1% level, and other characters were not different. Genetic analysis showed that the green-revertible albino trait was controlled by a single recessive nucleic gene. Using 209 recessive mutant individuals in the F2 population derived from the cross Pei＇ai 64S × Qiufeng M, a gene, tentatively named gra（t）, was located between the SSR markers of RM475 and RM2-22 on the long arm of chromosome 2. The genetic distance were 17.3 cM and 2.9 cM respectively.

Variation of Protein Content in Tartary Buckwheat Seeds

[Objective]The aim was to select tartary buckwheat materials with high protein content.[Method]Used 35 kinds of tartary buckwheat as experimental material,and the protein content of seeds had been determined.[Result]The protein content of 35 kinds of tartary buckwheat will change in the range of 23.65-193.28 mg/g with an average of 111.85 mg/g.There was difference among different origins of tartary buckwheat.The seeds from Hezhang in Guizhou and Sichuan had highest protein content,while the seeds from Nayong had lowest protein content.[Conclusion]The study had provided theoretical basis for the further study on the genetic and variation law of protein content in different tartary buckwheat resources.

Effects of mutation on a new strain Leptospirillum ferriphilum YXW and bioleaching of gold ore

Leptospirillum ferriphilum YXW was isolated through serial dilution from mixed microorganisms enriched in AMD from Dexing copper mine in Jiangxi Province, China. It was mutated by ultrasonic, UV and microwave to collect more efficient strain for bioleaching of gold ore. Physiological and biochemical characteristics indicate that strain YXW is a strict chemoautotrophic microorganism, and the optimal condition for its growth is temperature of 40 °C and pH 1.5. After mutation by ultrasonic, UV and microwave, the density of bacterial cells reached 9×109, 8.4×109 and 4.3×108 mL-1, increased by 291%, 265%and 87%, respectively, compared with the original culture. The bacterial total protein activity was improved by microwave and UV mutations, but was reduced by ultrasonic. Mutations had effects on bioleaching of gold ore in sequence of microwave〉UV〉ultrasonic. During gold ore bioleaching, the bacterial mutant after mutation by microwave had the best effect on the extraction rates of arsenic and iron, which were 19.6%and 17.7%higher than that of the original strain after bioleaching for 10 d, respectively. The results suggested that the effects of mutation on bioleaching of gold ore may not be mainly due to increase of bacterial cells density, but may be mainly attributed to the improvement of bacterial total protein activity.

Chloroplast Composition and Structural Differences in a Chlorophyll-reduced Mutant of Oilseed Rape Seedlings

对黄化突变体Cr352 9和野生型油菜 (BrassicanapusL .) 352 9叶绿体的超微结构和组成进行了比较。与野生型相比 ,突变体Cr352 9叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量。突变体的Chla/Chlb比值较高 ,是野生型的 2倍。电泳结果表明 ,突变体类囊体膜中LHCⅡ和其三聚体LHCⅡ 的含量减少。SDS_PAGE分析显示 ,LHCⅡ的脱辅基蛋白在突变体类囊体膜中明显减少。免疫印迹进一步表明 ,所有LHCⅡ组分的含量仅为野生油菜的类囊体膜的 1 / 3。突变体Cr352 9的天线系统比野生型 352 9的小。

Purification and Characterization of Cr-containing Nitrogenase Component Ⅰ

A mutant UW 3, which is unable to fix N 2 in the presence of Mo (Nif -) but can undergo phenotypic reversal to Nif + under Mo_deficient conditions, was able to grow in Cr_containing but Mo_ and NH 3_deficient medium. A partly purified nitrogenase component Ⅰ protein obtained from UW 3 grown on the Cr_containing medium was shown to contain Fe and Cr (atom ratio of Fe to Cr and Mo to Cr: 11.60 and 0.41) and to have 70% of the C 2H 2_ and H +_reduction activity of MoFe protein from the wild_type strain of Azotobacter vinelandii Lipmann. The Cr_containing protein was different in subunit composition from that of MnFe protein purified from the mutant strain grown in the presence of Mn, but similar to that of MoFe protein, that is, it was a tetramer composed of two different subunits (α 2β 2). The preliminary results indicated that the Cr_containing protein might be a nitrogenase component Ⅰ protein.

Genetic Screening of the Lipoprotein Lipase Gene for Mutations in Chinese Subjects with or without Hypertriglyceridemia

Objective： To investigate the association between the mutations in lipoprotein lipase gene and hypertriglyceridemia （HTG）. Methods： The lipoprotein lipase （LPL） gene was screened for mutations in 386 Chinese subjects with （108 cases in the HTG group） or without HTG （278 cases in the control group）, by single-strand conformation polymorphism （SSCP） analysis and DNA sequencing. Results： One novel silent mutation L103L, one missense mutation P207L, three splicing mutations Int3/3＇ -ass/C（-6）→T, and the common S447X polymorphism has been identified in the whole coding region and exon-intron junctions of the LPL gene were examined. Heterozygous P207L found in the HTG group was the first case reported in Asia and subsequently another P207L heterozygote was found in the proband＇s family, all of which suggested that P207L was one of the causes of familial combined hyperlipidemia, but was not so prevalent as that in French Canadian. Int3/3＇-ass/C（-6）→T was found in both groups in the present study although it was regarded as a pathogenic variant to HTG earlier on. Moreover about the beneficial polymorphism S447X, there was also some supportive evidence that the levels of triglycerides （TG） in S447X carriers were significantly lower than noncarders in the subjects without HTG. Conclusions： The association between the LPL variants and HTG is quite complicated and versatile, genotyping of LPL in a larger-scale screening should be necessary and justifiable.

Auto-Bcklund transformation and exact solutions of Wick-type stochastic Burgers equation

Burgers equation in random environment is studied. In order to give the exact solutions of random Burgers equation, we only consider the Wick-type stochastic Burgers equation which is the perturbation of the Burgers equation with variable coefficients by white noise W（t）=Bt, where Bt is a Brown motion. The auto-Baecklund transformation and stochastic soliton solutions of the Wick-type stochastic Burgers equation are shown by the homogeneous balance and Hermite transform. The generalization of the Wick-type stochastic Burgers equation is also studied.

Storage dynamics of fallen trees in a mixed broadleaved and Korean pine forest

A study of the storage dynamics in the mixed broadleaved and Korean pine forests was carried out in the Changbai Mountains, Jilin Province, P. R. China. The modifying law of fallen trees was the storage dynamics of the existing fallen trees and the annual input in the mixed broadleaved and Korean pine forest. The current storage of fallen trees was 16.25 t昲m-2 in the initially, but after 100 years, 85% of the storage in dry weight was decomposed, and little material was left after 300 years. The average annual input of fallen trees was 0.6 t昲m-2and it increased with time to 31.0 t昲m-2after 200 years, which was maintained until the climax community ended. The total storage of fallen trees increased in the early stage. The decomposition of fallen trees eventually reached equilibrium with storage being identical with the annual input of fallen trees.

Processing map and hot deformation mechanism of novel nickel-free white copper alloy

Hot compression test of a novel nickel-free white alloy Cu?12Mn?15Zn?1.5Al?0.3Ti?0.14B?0.1Ce (mass fraction, %) was conducted on a Gleeble?1500 machine in the temperature range of 600?800 °C and the strain rate range of 0.01?10 s?1. The constitutive equation and hot processing map of the alloy were built up according to its hot deformation behavior and hot working characteristics. The deformation activation energy of the alloy is 203.005 kJ/mol. An instability region appears in the hot deformation temperature of 600?700 °C and the strain rate range of 0.32?10 s?1 when the true strain of the alloy is up to 0.7. Under the optimal hot deformation condition of 800 °C and 10 s?1 the prepared specimen has good surface quality and interior structure. The designed nickel-free alloy has very similar white chromaticity with the traditional white copper alloy (Cu?15Ni?24Zn?1.5Pb), and the color difference between them is less than 1.5, which can hardly be distinguished by human eyes.

A Dendroclimatic Study of Relic Picea meyeri in Inner Mongolian Steppe

Dendroclimatic methods were used to investigate the fundamental relationships between the temperature variables and the growth of climatically relic Picea meyeri Rehd. et Wils. on sandy land of Inner Mongolian grasslands in the Xilin River Basin. The annual mean temperature and accumulated temperature (above 5 ℃, 10 ℃) respectively showed no significant correlation with the spruce growth relative to summer mean temperature, which displayed strong coherence with tree growth ( P <0.01). The mean temperature in May showed significantly negative correlation with spruce growth ( P <0.05). Furthermore, it was revealed that the negative influence of May temperature was due to monthly mean maximum temperature ( T max ) ( P <0.01) other than monthly mean minimum temperature ( T min ) values ( P <0.1), which indicated that the use of T min and T max separately can allow much more insights on the temperate influence. In addition, extreme maximum temperature in May and June might impose the most detrimental influence on tree growth in semi_arid Inner Mongolian grassland. The analysis of the recorded meteorological data demonstrated that the increases in temperature were synchronous with slight decreases in precipitation in the growing season, which suggested that P. meyeri may reduce growth due to temperature_induced drought. On the other hand, the analysis for decadal periods explored that trees appeared to reduce the sensitivity to the warming, and consequently increased sensitivity to rainfall. This may serve as a baseline for more accurate predictions of the potential impacts of altered climate regimes on tree growth.

Developmental Changes of IGF-Ⅰ mRNA Expression Level in Sheep Muscle

[ Objective] To detect the mRNA of muscle insulin-like growth factorl （IGF-I） in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. [ Method] With real-time quan- titative PCR, the muscle IGF-I mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. [ Result] The IGF-I mRNA in sheep muscle first increased and then decreased with ages, peaking at 30 days old in Kazak sheep and at 60 days old in Xinjiang fine-wool sheep. The IGF-I expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 or 90 days old （ P 〉0.05）, but was lower than that of the latter with extremely significant difference （ P 〈0.01 ） from 30 to 60 days old. [ Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscular IGF-I mRNA, but the expression level is different between these two species.

高科技电影手段的艺术魅力

电影是科学技术发展的产物,这是众所周知的不争事实。由电影诞生至今,随着科学技术的发展,由无声变成了有声,由黑白变成了彩色,由单声变成了立体声;各种蒙太奇都出现了,长镜头出现了,特技也出现了;广角镜被运用,变焦被运用……电影的镜头语言和表现手段得到了极大的丰富。

On Spatial Variability and Distribution of Soil Nutrients in Different Nitraria tangutorum Bobr.Populations in Tsaidam Basin

In this paper,six important distribution areas of Nitraria tangutorum Bobr.in Tsaidam Basin were selected as research objects,to study spatial variability and distribution of soil nutrients in different N.tangutorum populations and analyze the relationship between soil nutrient contents and geographical location,by measuring soil pH and the contents of organic matter（OM）,total nitrogen（N）,total phosphorus（P）,total potassium（K）,hydrolysis N,available P and available K in soils.Results showed that：（1） soil nutrient contents among different populations showed significant spatial variability,and soil depth had a significant effect on soil nutrients contents,but the variation rules were not obvious.（2）Average pH and average contents of OM,total N,total P,total K,hydrolysis N,available P and available K in soils with different depths（0-15,15-30,30-45 cm） varied in the range of 8.37-9.21,3.34-20.68,0.18-1.21,0.35-0.75,16.12-22.04,5.13-553.28,1.10-52.54 and 103.83-562.28 mg/kg,respectively.（3） The analysis results of correlation between average values of pH and contents of nutrient indexes in soils with different depths（0-15,15-30,30-45 cm） showed that the correlation of these indexes were different.（4）OM and total N contents in soils with different depths（0-15,15-30,30-45 cm） all had a significant positive correlation with latitude and negative correlation with longitude and altitude,and the correlation of available P and available K contents in surface soils（0-15 cm） with latitude,longitude and altitude were significant positive,significant negative and significant negative,respectively;moreover,longitude and latitude also showed a significant impact on soil available K contents with the depth of 30-45 cm.In addition,comprehensive analysis result of nutrient contents showed that N.tangutorum populations in Huaitou Tala Town had the highest fertility,and the fertility levels of N.tangutorum populations in Chaka Town and Wulan Keke Town were relatively lower.

Overexpression and mutations of tumor suppressor gene p53 in hepatocellular carcinoma

AIMS To examine the prevalance of p53 mutations in hepatocellular carcinoma (HCC) from Chongqing area and the relationship between the p53 mutations and clinicopathological features of HCC,as well as the risk factors. METHODS The overexpression and point mutations of tumor suppressor gene p53 in 38 cases of HCC were detected by a sensitive antigen retrieval fluid (ARF) immunohistochemical method and polymerase chain re- action(PCR)-restriction fragment length polymorphism (RFLP),and single strand conformation polymorphism (SSCP)-silver staining analysis. RESULTS The results showed that 16 of 38 HCCs had positive p53 protein (42.1%),7 HCCs had p53 mutation at 249 (18.4 % ) and 2 HCCS had point muta- tion within exon 7 other than 249. Among 9 cases of HCC with mutations,8 cases demonstrated positive p53 protein,its coincidental rate was 88.9%. The overexpression and mutations of p53 were significantly related to the differentiation and metastasis of HCCs. The frequency of p53 mutations was consistent with high prevalence of HBV and a moderate aflatoxin B1 (AFB1) exposure in our area. CONCLUSIONS The results suggest that AFB1 acts synergistically with HBV in the generation of p53 mutations. Furthermore,dietary exposure to AFB1 may mainly contribute to the tumor specific mutation at codon 249,while HBV may account for other scattered mutations in HCC.

特殊试纸的制备

借助试纸颜色的变化以测试物质的性质,有简易、迅速、方便的特点。以下介绍几种常见特殊用途的试纸的制备和作用。 1 乙酸铅试纸（白色） 将3g乙酸铅溶于100mL水中,放入滤纸,浸透取出,放在无H2S环境中晾干。 遇H2S时由白变黑,检验痕量的H2S。 2 淀粉试纸（白色） 将0.5g可溶性淀粉放入小烧杯中,加5mL水调成糊状,搅拌加入100mL沸水。