Nutritional factors influence regulation of growth hormone （GH）, insulin-like growth factor-Ⅰ （IGF-Ⅰ） and insulin （INS） in fish. But so far there are no published studies describing how single indispensable am...Nutritional factors influence regulation of growth hormone （GH）, insulin-like growth factor-Ⅰ （IGF-Ⅰ） and insulin （INS） in fish. But so far there are no published studies describing how single indispensable amino acids and different carbohydrate to lipid ratios influence those systems. Therefore, the present study aimed to evaluate whether arginine （Arg） intake and carbohydrate to lipid ratios would affect expression of GH, IGF-Ⅰ and INS in largemouth bass.展开更多
Anti-Mullerian hormone （amh） is one of the earliest functional genes expressed during testicular differentiation. It has been suggested that androgen signaling regulates critical genes for the differentiation and de...Anti-Mullerian hormone （amh） is one of the earliest functional genes expressed during testicular differentiation. It has been suggested that androgen signaling regulates critical genes for the differentiation and development of the testis. To elucidate the exact regulatory mechanisms involved in arnh transcription mediated by androgen signaling, androgen signaling was manipulated in zebrafish by cytochrome P450 17al （cyplTal） knockout and Flutamide treatment. In cyp17a1-deficient and Flutamide-treated testes, up-regulated sry-box9a （soxga） and down-regulated amh were observed. Moreover, a physical association of the zebrafish androgen receptor （AR） and SOX9A was found. The interaction between AR and SOX9A was mediated via the DNA binding domain （DBD） of AR and the transactivation domain （TA） of SOX9A, and was further enhanced by 5-alpha dihydrotestosterone （DHT）, one of the most potent androgens. Intriguingly, together with SOX9A, androgen signaling synergistically promoted amh transcription, mainly through the proximal 1 kb of the amh promoter region. Taken together, our data demonstrate a critical mechanism underlying the direct synergy of androgen signaling and SOX9A in the regulation of amh transcription.展开更多
文摘Nutritional factors influence regulation of growth hormone （GH）, insulin-like growth factor-Ⅰ （IGF-Ⅰ） and insulin （INS） in fish. But so far there are no published studies describing how single indispensable amino acids and different carbohydrate to lipid ratios influence those systems. Therefore, the present study aimed to evaluate whether arginine （Arg） intake and carbohydrate to lipid ratios would affect expression of GH, IGF-Ⅰ and INS in largemouth bass.
基金supported by the National Natural Science Foundation of China (31501857 to G.Z.and 31530077 to Z.Y.)the National Basic Research Program of China (2014CB138602 to Z.Y.)
文摘Anti-Mullerian hormone （amh） is one of the earliest functional genes expressed during testicular differentiation. It has been suggested that androgen signaling regulates critical genes for the differentiation and development of the testis. To elucidate the exact regulatory mechanisms involved in arnh transcription mediated by androgen signaling, androgen signaling was manipulated in zebrafish by cytochrome P450 17al （cyplTal） knockout and Flutamide treatment. In cyp17a1-deficient and Flutamide-treated testes, up-regulated sry-box9a （soxga） and down-regulated amh were observed. Moreover, a physical association of the zebrafish androgen receptor （AR） and SOX9A was found. The interaction between AR and SOX9A was mediated via the DNA binding domain （DBD） of AR and the transactivation domain （TA） of SOX9A, and was further enhanced by 5-alpha dihydrotestosterone （DHT）, one of the most potent androgens. Intriguingly, together with SOX9A, androgen signaling synergistically promoted amh transcription, mainly through the proximal 1 kb of the amh promoter region. Taken together, our data demonstrate a critical mechanism underlying the direct synergy of androgen signaling and SOX9A in the regulation of amh transcription.
