白山片的薄层色谱鉴别方法研究

目的建立白山片中白鲜皮、山豆根、北豆根的鉴别方法。方法采用薄层色谱法对白山片处方中主要的3味中药白鲜皮、山豆根、北豆根进行定性鉴别。结果各供试品色谱中,在与对照色谱相应的位置上显相同颜色的斑点,色谱清晰、无杂质斑点干扰。结论本文所制定的方法专属性强,重复性好,可有效地控制白山片的质量。

纯中药白山片抗小鼠肺癌作用的形态学研究

观察了抗肺癌纯中药白山片对小鼠移植瘤Ｌｅｗｉｓ肺癌的抑制作用及超微病理改变。结果：实验组的肿瘤重量明显减少，统计抑制率为４０％～６１．６％。在超微结构上，白山片破坏癌细胞的膜系统，使癌细胞裂解、崩溃；诱导癌细胞内产生大量溶酶体，致使其呈溶解变性坏死；激活淋巴细胞增生，观察到自然杀伤性淋巴细胞攻击破坏癌细胞。

中药白山片抗肺癌作用的实验研究

白山片具有清热解毒，扶正祛邪，化瘀散结和提高机体免疫力等功能，本文观察了白山片对小鼠移植瘤Ｌｅｗｉｓ 肺癌的抑制作用。实验组的肿瘤明显缩小，抑制率为４０ ％ ～６１ ．６ ％ ，而阳性对照组５ －氟脲嘧啶抑制率为５０ ．２ ％ ，在超微结构上白山片激活淋巴细胞增生观察到自然杀伤性淋巴细胞攻击破坏癌细胞，破坏癌细胞膜系统，使癌细胞裂解、崩溃，诱导癌细胞内产生大量溶酶体，使其溶解、变性、坏死。

中药白山片抗肺癌作用的疗效观察和实验研究

白山片，具有清热解毒、扶正祛邪、化瘀散结，促使癌瘤缩小或消失和提高机体免疫等功能。用白山片治疗中晚期肺癌１０３例，结果，总缓解率为８５％，总有效率为９６％。动物实验建立起小白鼠Ｌｅｗｉｓ肺癌模型，实验结果，５－氟脲嘧啶阳性对照组抗肺癌抑制率为５０．２％，白山片抗肺癌抑制率高达６１．６％。

中药“白山片”和“消结灵”能直接杀死癌细胞

北京市解放军304医院刘今方副主任医师,经20多年的苦心研究成抗肺癌的“白山片”和抗肝、胃、食道、结肠、乳腺癌的“消解灵”,在临床上取得确切的疗效。中国医科大学著名电镜专家高俊峰教授用该药对肿瘤的抑制作用,超微病理改变和抗癌机理进行了研究,他先后建立数百个动物模型,电子显微镜拍摄的照片表明,该药对癌细胞杀伤破坏途径有3条:1。

HPLC法测定白山片中苦参碱的含量

目的：建立以高效液相色谱法测定白山片中苦参碱含量的方法。方法：色谱柱为氨基键合柱（200mm×4．6mm，10μm），流动相为乙腈-0．5％磷酸溶液乙醇（100：8：10），检测波长为220nm，流速为1．0mL·min，枉温为30℃。结果：苦参碱工壹样量在0．27～8．66μg范围内与峰面积积分值呈良好的线性关系（r=0．9998）；平均加样回收率为99．52％，RSD=0．91％（n=6）。结论：本方法简便、准确、重现性好，可用于白山片的质量控制。

Effect of water stress on N_2O emission rate of 5 tree species

The N2O emission rates, photosynthesis, respiration and stomatal conductance of the dominant tree species from broadleaf/Korean pine forest in Changbai Mountain were measured by simulated water stress with the closed bag-gas chromatography. A total of five species seedlings were involved in this study, i.e.,Pinus koraiensis Sieb. et Zucc,Fraxinus mandshurica Rupr,Juglans mandshurica Maxim,Tilia amurensis Rupr, andQuercus mongolica Fisch. ex Turcz.. The results showed that the stomatal conductance, net photosynthetic rate and N2O emission of leaves were significantly reduced under the water stress. The stoma in the leaves of trees is the main pathway of N2O emission. N2O emission in the trees mainly occurred during daytime. N2O emission rates were different in various tree specie seedlings at the same water status. In the same tree species, N2O emission rates decreased as the reduction of soil water contents. At different soil water contents (MW, LW) the N2O emission rates ofPinus koraiensis decreased by 34.43% and 100.6% of those in normal water condition, respectively. In broadleaf arbor decreased by 31.93% and 86.35%, respectively. Under different water stresses N2O emission rates in five tree species such asPinus koraiensis, Fraxinus mandshurica, Juglans mandshurica, Tilia amurensis, andQuercus mongolica were 38.22, 14.44, 33.02, 16.48 and 32.33 ngN2O·g?1DW·h?1, respectively. Keywords Trees - N2O emission rate - Soil water stress - broadleaf/Korean pine forest - Changbai Mountain CLC number S718.55 Document code A Foundation item: This project was supported by the National Natural Science Foundation of China (No. 30271068), the grant of the Knowledge Innovation Program of Chinese Academy of Sciences (KZ-CX-SW-01-01B-10), and the Special Funds for Major State Basic Research Program of China (No. G1999043407)Biography: Wang Miao (1964-), male, associate professor in Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110016, P. R. China.Responsible editor: Song Funan