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抗生素诱导SPF级BALB/c小鼠胃肠道白念珠菌定植模型 被引量:1
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作者 黄雪峰 袁静 魏泓 《世界华人消化杂志》 CAS 北大核心 2006年第12期1161-1166,共6页
目的:建立抗生素诱导SPF级BALB/c小鼠胃肠道白念珠菌定植模型.方法:SPF级♀Balb/c小鼠随机饮用抗生素头孢曲松水溶液5d(120h)后,单次口服灌胃107CFU(50μL)白念珠菌以诱导其定植.并运用平板计数和基于细菌16SrDNA的PCR-DGGE技术分析小... 目的:建立抗生素诱导SPF级BALB/c小鼠胃肠道白念珠菌定植模型.方法:SPF级♀Balb/c小鼠随机饮用抗生素头孢曲松水溶液5d(120h)后,单次口服灌胃107CFU(50μL)白念珠菌以诱导其定植.并运用平板计数和基于细菌16SrDNA的PCR-DGGE技术分析小鼠胃肠道的白色念珠菌定植与微生物区系变化.结果:抗生素处理5d后,减少了胃肠道99.99%以上的可培养厌氧菌和肠杆菌,与处理前相比,有极显著差异(厌氧菌:8.53±0.31Log10CFU/gvs4.18±0.90Log10CFU/g,P<0.01;肠杆菌:3.67±0.14Log10CFU/gvs0,P<0.01),而且DGGE图谱分析显示,抗生素处理后小鼠细菌微生物区系的多样性明显减少(条带数由27-32条减少到2-7条).对抗生素处理小鼠单次口服灌胃107CFU白念珠菌2d后,在小鼠胃、小肠、盲肠和大肠检测到大量的C.albicans,数量分别为4.44±0.02Log10CFU/tissue,5.05±0.19Log10CFU/tissue,5.62±0.06Log10CFU/tissue,4.95±0.14Log10CFU/tissue,并且单次口服灌胃白念珠菌1wk后,小鼠胃肠道内仍维持有4.01±0.06Log10CFU/g的白念珠菌定植.与正常Control组和Antibioticonly组相比,Antibiotic+Candida模型组小鼠肠杆菌增殖了10到100倍(3.65±0.16Log10CFU/g,3.21±0.18Log10CFU/gvs5.42±0.33Log10CFU/g,P<0.05);同时DGGE图谱分析显示,Antibiotic+Candida模型组小鼠细菌微生物区系多样性较低(条带数Antibiotic+Candida/22-24条,Control/28-34条和Antibioticonly/27-34条),各小鼠细菌微生物区系之间的相似性为63.8%-67.0%.结论:抗生素处理诱导了胃肠道微生物区系紊乱,导致了白念珠菌在SPF级Balb/c小鼠胃肠道定植成模. 展开更多
关键词 抗生素 白念珠菌定植 微生物区系
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Determination of Fungal Colonization in the Oral Cavity of College Students
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作者 Floridia Ricardo Rodriguez Graciela Ampuero Veronica Gonzalez Luis 《Journal of Life Sciences》 2012年第10期1133-1141,共9页
In recent years, an increase in opportunistic fungal colonization in the oral cavity in immunocompetent patients (IC) has been observed. In the bibliography, the most observable genre is Candida and less frequently ... In recent years, an increase in opportunistic fungal colonization in the oral cavity in immunocompetent patients (IC) has been observed. In the bibliography, the most observable genre is Candida and less frequently found are other opportunistic such as Aspergillus, Rhizopus, Cryptococcus, and others. The authors determined the presence of fungi in the oral cavity of IC students, and their relationship with the concentration of secretory IgA in saliva. To this end, we collected 50 samples of oral cavity swabs, which underwent direct examination and culture in Sabouraud dextrose agar with chloramphenicol. For its identification, CHROMagar Candida and API Candida (BioMerieux) were used. We obtained nine positive cultures (7 Candida albicans and 2 Saccharomyses cerevisiae), which represented 18% of the studied population. Throughout radial immunodiffusion (RID plates-PLATE), we determined the concentration of secretory IgA. No relationship was observed between the colonized group and group that was not colinized. The colonization rate found is below the one described in the bibliography (30% to 50%). However, these opportunistic fungi cause transitory colonization with no clinical relevance in IC patients and, its percentage can vary according to the studied population. 展开更多
关键词 Oral cavity IMMUNOCOMPETENT CANDIDA fungal colonization.
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