白斑方熏洗配合超声聚焦治疗上皮内非瘤样病变疗效观察

目的观察白斑方熏洗配合超声聚焦治疗上皮内非瘤样病变疗效。方法选取2014年2月至2016年2月在本院妇科门诊就诊的上皮内非瘤样病变患者136例，以自愿参组观察疗效为原则，用抽签方式分为治疗组、对照组各68例，治疗组采用中药白斑方熏蒸配合超声聚焦治疗；对照组单纯采用外阴超声聚焦治疗。自行设计疗效统计表，把各患者的疗效分别登记并录入计算机，采用SPSS10．0软件进行统计分析。结果临床疗效观察统计发现，治疗组总有效率为82．35％，对照组为58．82％，两组疗效比较差异有统计学意义（P=0．0136）。结论自斑方熏洗配合超声聚焦治疗上皮内非瘤样病变疗效明显优于单纯采用外阴超声聚焦治疗，值得我们继续应用。

他克莫司软膏联合自拟白斑方治疗白癜风49例

我科于2007年6月—2009年3月局部外用0.1%他克莫司软膏联合中药治疗白癜风49例,取得较满意的疗效,现报告如下。

白斑方配合波姆光治疗女阴白色病变55例

白斑方配合波姆光治疗女阴白色病变５５例山东省临沂市中医院（２７６００２）袁悦明钟善文主题词外阴白色病变／外治法熏洗疗法按摩波姆光／照射外阴白色病变是妇科多发病，笔者于１９９４年至１９９６年治疗１００例，随机分为白斑方配合波姆光照射治疗组５５例，单纯波...

白斑方联合聚焦超声治疗外阴硬化性苔藓疗效观察

研究白斑方联合聚焦超声治疗外阴硬化性苔藓的临床疗效。由于临床数据反馈,外阴硬化性苔藓的患者数量逐渐增加,且临床治疗遇到瓶颈,深入开展研究,寻找最佳治疗方案,为临床外阴硬化性苔藓的治疗提供参考,希望能够早日减轻患者所受疼痛,缓解其心理压力,为临床医学作出贡献。方法:选取2020年9月-2021年9月我院收治的符合纳入标准的50例外阴硬化性苔藓患者作为本次研究对象,选用抽签法随机分为2组,对照组给予聚焦超声治疗,观察组全在对照组基础上添加白斑方坐浴治疗,比较两组患者的临床疗效、中医症候积分、生活质量评分。结果:观察组临床疗效、中医症候积分、治疗后生活质量评分均优于对照组(P<0.05)。结论:白斑方联合聚焦超声治疗外阴硬化性苔藓疗效显著,可以有效提高患者生活质量,减轻瘙痒和疼痛症状,使患者恢复正常工作和生活,临床应用价值极高。

白斑颗粒剂Ⅰ号方联合白斑膏治疗外阴白斑的临床疗效观察

目的白斑颗粒剂Ⅰ号方联合白斑膏治疗外阴白斑的临床疗效观察。方法选取2019年9月—2020年12月本院收治的外阴白斑患者80例作为研究对象,将其随机分为治疗组与对照组各40例。治疗组给予口服白斑颗粒剂Ⅰ号方、外涂白斑膏以及光疗照射波姆光治疗仪;对照组给予口服转移因子胶囊、外涂干扰素凝胶以及光疗照射波姆光治疗仪。观察2组临床疗效及中医证候评分的变化。结果治疗组的总有效率明显于对照组(95.0%比82.5%),差异有统计学意义(P<0.05);2组患者治疗后中医证候评分均较治疗前降低,且以治疗组降低更为显著[(2.03±1.24)比(3.45±1.29)],2组比较差异均有统计学意义(均P<0.05)。结论白斑颗粒Ⅰ号方联合白斑膏治疗外阴白斑有效。

白斑颗粒Ⅰ号方对白癜风患者Foxp3 mRNA表达的影响

目的观察白斑颗粒Ⅰ号方对白癜风患者Foxp3 m RNA表达的影响,阐明白斑Ⅰ号方治疗白癜风的作用机制。方法白癜风患者60例,随机分为试验组和对照组各30例,健康对照组30例,采用RT-PCR方法检测各组PBMC中Foxp3 m RNA表达变化情况。结果白癜风患者组Foxp3 m RNA水平低于健康对照组,差异有统计学意义(P<0.05);实验组治疗3个月后Foxp3 m RNA水平高于治疗前,差异有统计学意义(P<0.05);对照组治疗3个月后Foxp3 m RNA水平高于治疗前,差异有统计学意义(P<0.05);治疗3个月后,实验组治疗前后外周血Foxp3 m RNA升高值高于对照组治疗前后外周血Foxp3 m RNA升高值,差异有统计学意义(P<0.05)。结论白斑颗粒Ⅰ号可能通过上调调节T细胞功能,使PBMC Foxp3 m RNA恢复或接近正常水平达到治疗白癜风的目的。

自体表皮移植联合中药白斑颗粒剂Ⅰ方治疗白癜风的疗效及复发率的临床观察

白癜风是一种获得性皮肤色素异常，表现为脱色性白斑，是皮肤科常见病，严重影响患者心理健康。自体表皮移植是治疗稳定期白癜风的一种较好方法，在我院开展近20年，疗效确切。上海最新的一次大样本流行病学调查显示自体表皮移植的总有效率为92．7％。但少数患者移植治疗半年以后，原复色皮片再次脱色。笔者应用自制中药白斑颗粒剂I方联合自体表皮移植术治疗白癜风。

外阴白斑的中医治疗

外阴白斑是外阴色素减退性疾病。根据白斑患者临床症状不同,辨证分为肝肾阴虚型和脾肾阳虚型。以滋养肝肾、补益精血、温肾健脾壮阳法组方内服,并予白斑外洗方外洗,辅以微米光照射治疗,临床取得良好疗效。

中药熏洗联合康妇凝胶外用治疗外阴色素减退性疾病的临床疗效研究

分析研究中药熏洗联合康妇凝胶外用治疗外阴色素减退性疾病的临床疗效。方法 将2022年4月-2023年4月在我科就诊的60例外阴色素减退性疾病患者按照随机数字表法随机分为两组,对照组仅外用康妇凝胶,观察组外用康妇凝胶加白斑外洗方外阴熏洗。观察两组患者的治疗效果。结果 治疗后观察组患者的临床症状综合积分为(2.05±1.23),对照组患者的临床症状综合积分为(3.62±1.08),比较差异具有统计学意义(P<0.05);观察组患者治疗有效率96.67%,明显高于对照组治疗有效率66.67%(P<0.05);治疗后6个月后,观察组患者复发率3.33%,对照组患者复发率16.67%,比较差异具有统计学意义(P<0.05)。结论 外阴白斑外洗方联合康妇凝胶外用治疗外阴色素减退性疾病,具有操作方法简便、疗效稳定、复发率低等优点,具有良好的临床效果,可推广应用于临床。

中西医结合外治法治疗女性外阴硬化性苔藓疗效观察

目的:研究中西医结合外治法治疗女性外阴硬化性苔藓的临床疗效。方法:治疗组60例,中药外洗(白斑1号方、白斑2号方)、外涂(白斑散),结合外阴皮下注射(维生素B1、维生素B12),实施综合治疗1个月。对照组60例,无中药外涂及外阴皮下注射,其它治疗同试验组。结果:治疗组60例中痊愈46例,总有效率100%。对照组中痊愈15例,总有效率75%。病理组织及阴道镜检查前后对比也有明显好转。两组相比差异有统计学意义(P<0.05)。结论:中西医结合外治法治疗外阴硬化性苔癣疗效明显。

Proteomic Analyses of the Shrimp White Spot Syndrome Virus

White spot syndrome virus (WSSV), a unique member within the virus family Nimaviridae, is the most notorious aquatic virus infecting shrimp and other crustaceans and has caused enormous economic losses in the shrimp farming industry worldwide. Therefore, a comprehensive understanding of WSSV morphogenesis, structural proteins, and replication is essential for developing prevention measures of this serious parasite. The viral genome is approximately 300kb and contains more than 180 open reading frames (ORF). However, most of proteins encoded by these ORF have not been characterized. Due to the importance of WSSV structural proteins in the composition of the virion structure, infection process and interaction with host cells, knowledge of structural proteins is essential to understanding WSSV entry and infection as well as for exploring effective prevention measures. This review article summarizes mainly current investigations on WSSV structural proteins including the relative quantities, localization, function and protein-protein interactions. Traditional proteomic studies of 1D or 2D gel electrophoresis separations and mass spectrometry (MS) followed by database searches have identified a total of 39 structural proteins. Shotgun proteomics and iTRAQ were initiated to identify more structural proteins. To date, it is estimated that WSSV is assembled by at least 59 structural proteins, among them 35 are defined as the envelope fraction (including tegument proteins) and 9 as nucleocapsid proteins. Furthermore, the interaction within several major structural proteins has also been investigated. This identitification and characterization of WSSV protein components should help in the understanding of the viral assembly process and elucidate the roles of several major structural proteins.

Identification and Characterization of Nuclear Localization Signals within the Nucleocapsid Protein VP15 of White Spot Syndrome Virus

The nucleocapsid protein VP15 of white spot syndrome virus (WSSV) is a basic DNA-binding protein. Three canonical bipartite nuclear localization signals (NLSs), called NLS1 (aa 11-27), NLS2 (aa 33-49) and NLS3 (44-60), have been detected in this protein, using the ScanProsite computer program. To determine the nuclear localization sequence of VP15, the full-length open reading frame, or the sequence of one of the three NLSs, was fused to the green fluorescent protein (GFP) gene, and transiently expressed in insect Sf9 cells. Transfection with full-length VP15 resulted in GFP fluorescence being distributed exclusively in the nucleus. NLS1 alone could also direct GFP to the nucleus, but less efficiently. Neither of the other two NLSs (NLS2 and 3) was functional when expressed alone, but exhibited similar activity to NLS1 when they were expressed as a fusion peptide. Furthermore, a mutated VP15, in which the two basic amino acids (11RR12) of NLS1 were changed to two alanines (11AA12), caused GFP to be localized only in the cytoplasm of Sf9 cells. These results demonstrated that VP15, as a nuclear localization protein, needs cooperation between its three NLSs, and that the two residues (11RR12) of NLS1 play a key role in transporting the protein to the nucleus.

Optimum Fish for Fish Glue Processing for Fish Skin Value Added and the Glue Character

Fish glue is the collagen from scale skin and bone of fish. It is known for value added product from fish processing and the adhesive agent for wood paper book binding etc. This work was aimed with the method to produce fish glue from fish skin through representing optimum fish type and its glue character by study on standard method for fish glue processing （using Oreochromis niloticus skin as the model）, optimum fish type and characterization of the glue from optimum fish skin. Soaking fish skin in 0.1 N NaOH for 6 h and changed it every 3 h for pretreatment before acid extraction with 0.5 M acetic acid was the optimum condition for the standard processing. Among 20 types （O. niloticus, Oreochromis sp., Nemipterus sp., Psettodes erumei, Epiephelus malabaricus, Sphyraena obstsata, Channa striata, Xenentodon cancila, Barbonymus gonionotus, Liza vaigiensis, Anabas testudineus, Chanos chanos, Micronema bleekeri, Thunnus tonggol, Rastrelliger brachysom, Epinephelus lanceolatus, Lutjanus lineolatus, Pomadasys hasta, Selar crumenophthalmus and Sardaorentalis sp.）, Chanos chanos was an appropriate type for glue production. Fat, protein, carbohydrate, moisture, pH and viscosity of Chanos chanos glue were 0.32%, 4.23%, 83.8%, 11.56%, 3.35, 4,978.33-8,180 cp, respectively. The glue was collagen type I which was composed ofal （148 kDa） and a2 （129 kDa） chain and could bind paper, wood and foam sheet.