三白明目片治疗“中浆”病80例

采用三白明目片(白茅根、白蒺藜、茯苓、白术、车前子、赤芍等)治疗中心性浆液性脉络膜网膜病80例,总有效率为93.88％,疗效显著优于对照组(P<005)。提示本法具有健脾利水、活血祛瘀、清肝明目之功效。对治疗中浆病可缩短程,减少复发。

深情寓丹青——记著名书画家白明

在中华民族大家庭里,内蒙古(蒙古族)同黄河流域地区一样,同是中华民族文明最早的发源地之一,有着古老而悠久的历史.本文的主人翁白志明,这位原国家副总理乌兰夫的秘书,就是两千万内蒙古人民的骄子.他为国家为人民尽自己的忠心,支撑着一种精神--中华精神.他还一直坚持书画艺术创作和教学,广览精采,中得心源,且是当代著名书画家.

白明高速公路水泥稳定碎石基层施工养生技术探讨

本文通过多种养生方式的对比研究,提出白明高速公路水泥稳定碎石基层可行的养生方式、方法,对于干旱沙漠地区公路路面基层施工具有重要的借鉴意义。

白明自述

我向往简单的丰富，雅致的静穆。

绿茶 萨克斯 旷野——白明和他的陶瓷艺术

《收藏·拍卖》杂志创刊号即将发排，编辑部约我介绍一位当代优秀的陶艺家和他的作品。我接稿时不觉有何困难，但待动笔时却踌躇起来，不知从何下笔，原因是中国当代最优秀的陶艺家我认识十之八九，这位陶艺家写谁呢?

“知道”、“明白”类动词与疑问形式

"知道、明白"类动词阻碍疑问语气从宾语从句上升到主句,是非疑问形式,不过它们容易形成非典型疑问句。这类动词属于"具有知识"事件,一般可以带疑问从句和陈述从句,但涉及主语的能力与意愿时不能带陈述从句;"否定达到答案"时可以带疑问从句和陈述从句,而"否定澄清问题"时不能带陈述从句。

Effects of Phosphate and Lime Application on Growth of Trifolium repens,Chamaecrista rotundifolia and Macroptilium atropurpureum in Red Soils

[Objective] The aim was to research effects of P fertilizer and lime on growth of Trrifolium repens, Chamaecrista rotundifolia and Macroptilium atropur- pureum, to provide references for cultivation of the three plants. [Method] Pot experiments were conducted with Trrifolium repens, Charnaecrista rotundifolia and Macroptilium atropurpureum in 2010 in order to research effects of lime and P fer-tilizer mixture on growth of the plants in southern hilly acidic red soils. [Result] With lime amount fixed, application of P fertilizer would enhance plant height, total tiller number and dry matter. When P fertilizer was not applied, however, plant height of the three plants achieved the peak by lime at 1.4 g/kg which proved best for improvement of acidity of red soils. With P fertilizer at 200 mg/kg was applied, biomass of Trifolium repens and Macroptilium atropurpureum achieved the highest by lime at 2.1 g/kg, but total biomass of Chamaecrista rotundifolia was the highest by lime at 1.4 g/kg. [Conclusion] The research provides references for planting and production of Trifolium repens, Chamaecrista rotundifolia and Macroptilium atropur-pureum in southern hilly regions.

Improvement of the Hydroponic Growth and Waterlogging Tolerance of Petunias by the Introduction of vhb Gene

The coding sequence of Vitreoscilla hemoglobin (vhb) was cloned with PCR technique from Vitreoscilla stercoraria Pringsheim. The plant expression vector with vhb gene under the control of CaMV 35S promoter was constructed and used in the transformation of Petunia hybrida Vilm by the Agrobacterium mediated procedure. The results of PCR amplification and Southern hybridization indicated that the vhb gene had been integrated into the petunia genome and the vhb gene expression had been detected by RT-PCR amplification. In hydroponic culture the transgenic petunias grew much better than non-transgenic controls. For further analysis of hypoxia tolerance of transgenic petunia, the petunia plants with vhb gene were submerged into liquid MS medium. The transgenic plants survived in hypoxic condition and grew out of the liquid surface in a few weeks, while non-transgenic plants were still submerged and suffocated in culture solution without ability to grow out of liquid medium in submersed culture for four to five weeks. The vhb gene transformed petunia plants had been planted and tested in a simulated flooding condition, and showed obvious tolerance to water-logging. It seen is that hemoglobin gene from Vitreoscilla might have the potential use in molecular breeding for the improvement of plant resistance to hypoxia and flooding.

Enhanced migration of tissue inhibitor of metalloproteinase overexpressing hepatoma cells is attributed to gelatinases: Relevance to intracellular signaling pathways

AIM: To study the effect of gelatinases (especially MMP-9) on migration of tissue inhibitor of metalloproteinase (TIMP-1) overexpressing hepatoma cells. METHODS: Wild type HepG2 cells, cells stably transfected with TIMP-1 and TIMP-1 antagonist (MMP-9-H401A, a catalytically inactive matrix metalloproteinase (MMP) which still binds and neutralizes TIMP-1) were incubated in Boyden chambers either with or without Galardin (a synthetic inhibitor of MMP-1, -2, -3, -8, -9) or a specific inhibitor of gelatinases. RESULTS: Compared to wild type HepG2 cells, the cells overexpressing TIMP-1 showed 115% migration (P<0.05) and the cells overexpressing MMP-9-H401A showed 62% migration (P<0.01). Galardin reduced cell migration dose dependently in all cases. The gelatinase inhibitor reduced migration in TIMP-1 overexpressing cells predominantly. Furthermore, we examined intracellular signal transduction pathways of TIMP-1-dependent HepG2 cells. TIMP-1 deactivates cell signaling pathways of MMP-2 and MMP-9 involving p38 mitogen-activated protein kinase. Specific blockade of the ERK pathway suppresses gelatinase expression either in the presence or absence of TIMP-1. CONCLUSION: Overexpressing functional TIMP-1-enhanced migration of HepG2-TIMP-l cells depends on enhanced MMP-activity, especially MMP-9.

PROPERTIES OF PROTEOLYTIC TOXIN OF VIBRIO ANGUILLARUM FROM DISEASED FLOUNDER

Extracellular products (ECP) produced by Vibrio anguillarum strain M3 originally isolated from diseased flounder ( Paralichthys olivaceus ) were prepared. ECP of M3 showed gelatinase, casinase, amylase and haemolytic activity on agarose plates. High protease activity against azocasin was detected. Bacterium M3 showed highest growth and protease activity at 25℃. The protease present in ECP showed maximal activity at pH 8 and 55℃; was completely inactivated by application of 80℃ heat for 30 min; was completely inhibited by EDTA and HgCl 2, and was partially inhibited by PMSF, SDS, MnCl 2 and iodoacetic acid; but not inhibited by CaCl 2 and MgCl 2. The ECP was toxic to flounder fish at LD 50 values of 3.1 μg protein /g body weight. The addition of HgCl 2 and application of heat at 50℃ decreased the lethal toxicity of ECP. When heated at 100℃, ECP lethality to flounder was completely inhibited. After intramuscular injection of ECP into flounder, it showed evident histopathological changes including necrosis of muscle, extensive deposition of haemosiderin in the spleen, dilated blood vessels congested with numerous lymphocytes in the liver. These results showed that ECP protease was a lethal factor produced by the bacterium V. anguillarum M3.

Detection of gelatinase B activity in serum of gastric cancer patients

AIM： To determine the proteolytic activity and expression of gelatinase B in serum of gastric cancer patients and their correlation with the stage of the tumor. METHODS： Sera from 23 patients who underwent surgery for primary gastric cancer as the experimental group and from 11 as the control group were used to determine the proteolytic activity and its inhibition by EDTA and 1,10-phenanthroline. Gelatinase B activity was detected by SDS polyacrylamide gel electrophoresis （SDS-PAGE） and SDS-PAGE zymography. RESULTS： Proteolytic enzyme activity was increased in gastric cancer patients when compared to the control group （,0〈0.05）. The proteinases were determined to be metalloproteinases upon inhibition test with specific metalloproteinase inhibitors 1,10-phenanthroline （P〈0.05） and EDTA （P〈0.01）. SDS-PAGE and SDS-PAGE zymography revealed gelatinase B （proMMP-9） activity and its molecular mass of 92 ku. CONCLUSION： Proteinase activity is overexpressed in serum of gastric cancer patients. Gelatinase B in serum plays an important role in the progression of gastric cancer. ProMMP-9 can be used as a marker for invasiveness of gastric cancer.Proteolytic activity; Inhibition

Antioxidation Activities of Low-Molecular-Weight Gelatin Hydrolysate Isolated from the Sea Cucumber Stichopus japonicus

Gelatin extracted from the body wall of the sea cucumber （Stichopus japonicus） was hydrolyzed with flavourzyme. Low-molecular-weight gelatin hydrolysate （LMW-GH） of 700-- 1700 Da was produced using an ultrafiltration membrane bioreaetor system. Chemiluminescence analysis revealed that LMW-GH scavenges high free radicals in a concentration-dependent manner; IC50 value for superoxide and hydroxyl radicals was 442 and 285 μgmL-1, respectively. LMW-GH exhibited excellent inhibitory characteristics against melanin synthesis and tyrosinase activity in B16 cells. Furthermore, LMW-GH notably increased in- traeellular glutathione （GSH）, which in turn suppressed melanogenesis. LMW-GH performs antioxidation activity, holding the potential of being used as a valuable ingredient in function foods, cosmetics and pharmaceuticals or nutriceuticals.

Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle

Hyaluronan binding protein 1 (HABP1) is a negatively charged multifunctional mammalian protein with a unique structural fold. Despite the fact that HABP1 possesses mitochondrial localization signal, it has also been localized to other cellular compartments. Using indirect immunofluorescence, we examined the sub-cellular localization of HABP1 and its dynamics during mitosis. We wanted to determine whether it distributes in any distinctive manner after mitotic nuclear envelope disassembly or is dispersed randomly throughout the cell. Our results reveal the golgi localization of HABP1 and demonstrate its complete dispersion throughout the cell during mitosis. This distinctive distribution pattern of HABP1 during mitosis resembles its ligand hyaluronan, suggesting that in concert with each other the two molecules play critical roles in this dynamic process.

Composition of Starch and Protein in the Endosperm of Newly Generated Double Recessive Waxy and Opaque 2 Maize （Zea mays L.） Genotypes

Waxy maize with its pure amylopectin starch is the staple food of many ethnic minorities in hilly regions of Southeast Asia （SEA）. A combination of waxy and quality protein maize （QPM） traits would improve the quality of protein of waxy maize for human consumption. Double recessive waxy-QPM （wx-o2） genotypes had been generated from Southern Chinese material by haploid induction of crosses heterozygous for the two quality traits with an absolutely conserved waxy type and an improved amino acid profile. The vitreous kernel trait （due to the additional modifier genes present in QPM） was lost in the wx-o2 plant material; this may be due to the waxy mutation, this is anyhow desirable for acceptance as waxy maize is preferred due to its soft grains. The content of the quality limiting amino acid lysine was greatly increased in double recessive wx-o2 genotypes compared to standard waxy maize, but still with a high variation among genotypes for future improvement. Conclusively, it was indeed possible to combine two grain quality mutations successfully within one genotype and prototypes of double quality wx-o2 are available now to contribute to meet human requirements in essential amino acids and thus reduce malnutrition in various regions of Asia.

Vitreoscilla hemoglobin gene(vgb)improves lutein production in Chlorella vulgaris

Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the effi ciency of cell respiration and metabolism.In this study,we introduced a Vitreoscilla hemoglobin gene(vgb)into Chlorella vulgaris by Agrobacterium tumefaciens-mediated transformation(ATMT).PCR analysis confi rmed that the vgb gene was successfully integrated into the Chlorella vulgaris genome.Analysis of biomass obtained in shake fl asks revealed transformant biomass concentrations as high as 3.28 g/L,which was 38.81% higher than that of the wild-type strain.Lutein content of transformants also increased slightly.Further experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants,which was 36.77% higher than that of the wild-type strain.The above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris,with applications to lutein production from Chlorella during fermentation.

Simultaneous Expression of Vitreoscilla Globin Gene and Lytic Genes of Phage A in a Novel Recombinant Escherichia Coli Used for Production of PHB

Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage A with S amber mutation (S-RRz) and poly(B-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a same Escherichia coli cell, simultaneously or respectively. Six novel strains containing phbCAB and vgb with or without lytic genes were constructed. Strain VG1 (pTU14), in which vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB without S- RRz were not better than that of strains harbored ph&CAB only. The simultaneous expression of vgb and S- RRz in the recombinant VG1 (pTU14) showed a great potential for low-cost production of PHB.

Murine brain tissues with human cytomegalovirus infection: a proteomic study

To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus（HCMV） infection and paired murine brain tissues and to identify the differential expression proteins. Methods： Forty Kunming mice were randomly divided into infection group （20） injected with HCMVAD169 and control group （20） injected with saline into their brain. After 30 days, the murine brain tissues by HCMV infection and paired murine brain tissues were separated by two-dimensional gel electrophoresis（2-DE）, analyzed by Image Master 2D software, and identified by peptide mass fingerprint（PMF） and database searching, and make Western blotting analyses the differential expression of individual proteins. Results： Well resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry（MS） were matched in the SWISS-2D PAGE database, Western blotting analyses were further carried out to verify the differential expression of individual proteins. Conclusion： These data will be valuable for studying the diagnosis of disease at an early stage, mechanisms of pathogenic and the key to the development of anti-HCMV medicine.

动补结构“V+个+明白”的语义分析

动补结构“V+个+明白”自宋元时期出现以来,一直在口语中使用。本文从两个方面对该结构进行语义分析:首先说明“V+个+明白”中V的四种不同的语义类型,进而对每种类型进行语义分析,认为该结构包含同形异义的两种格式;然后把“V+个+明白”与“V+得+明白”进行对比,从而得出“V+个+明白”具有有界性、结果性和未然性的特征。

戏说白氏双雄

白磊与白明，这自家两兄弟，可说是现今中国画坛上引人注目的两颗星辰。

白花九里明抗菌和抗炎作用研究

目的：研究壮药白花九里明提取物的抗菌和抗炎作用,为开发新药提供实验依据。方法：抗菌采用琼脂扩散法、肉汤稀释法测定壮药白花九里明提取物对金黄色葡萄球菌（SA）、耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌（PA）、多重耐药铜绿假单胞菌（MDR-PA）、大肠埃希菌（E.coli）、产超广谱β-内酰胺酶大肠埃希菌（E.coli-ESBLs）、肺炎克雷伯菌（K.pne）、产超广谱β-内酰胺酶肺炎克雷伯菌（K.pne-ESBLs）的抑菌圈直径、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。抗炎采用二甲苯致小鼠耳肿胀、醋酸致小鼠毛细血管通透性增加、角叉菜致小鼠足肿胀、琼脂致小鼠肉芽肿模型研究。结果：壮药白花九里明提取物对8种细菌具有体外抑菌活性,对SA、MRSA作用最强;水提物、醇提物对8种细菌抑菌圈直径分别是10.03~23.17mm、8.27~19.75mm;水提物对8种细菌MIC：0.020~0.691g/ml,MBC：0.025~0.758g/ml;醇提物对8种细菌MIC：0.025~1.097g/ml,MBC：0.050~1.149g/ml,水提物作用强于醇提物。水提物13.4g/kg,26.8g/kg剂量组有显著的抑制二甲苯致小鼠耳肿胀和醋酸致小鼠毛细血管通透性增高的急性炎症作用;并有明显抑制角叉菜致小鼠足肿胀、琼脂致小鼠肉芽肿的急、慢性炎症作用。结论：壮药白花九里明提取物具有广谱体外抑菌活性,对耐药菌也具有抑菌活性,水提物具有良好的抗炎作用。