Objective Tu Jia ethnomedicine is a unique medical system inherited and adhibited by Tu Jia minority living in central-south China. Panax japonicus C. A. Mey.(Bai San Qi,白三七) is recognized as an effective and rare ...Objective Tu Jia ethnomedicine is a unique medical system inherited and adhibited by Tu Jia minority living in central-south China. Panax japonicus C. A. Mey.(Bai San Qi,白三七) is recognized as an effective and rare medicinal plant to treat weakness, fatigue and rheumatism in Tu Jia ethnomedicine. This paper is to discover more substance evidence for the application of Tu Jia ethnomedicine. Methods Column chromatography and preparative high performance liquid chromatography (HPLC) was applied for isolation and purification;1H-NMR, 13C-NMR, 1H-1H COSY, HSQC and HMBC NMR spectra were applied for structure identification;Methyl thiazolyl tetrazolim (MTT) assays were applied for cytotoxicity evaluation. Results Totally 12 known compounds were isolated by column chromatography and preparative HPLC from rhizomes of Panax japonicus C. A. Mey.(Bai San Qi,白三七). Structures of these compounds were identified by their NMR spectra. All the 12 compounds were triterpenoid saponins. Five of them were oleanolic acid type, and the remaining 7 were dammarane type. Eleven compounds were assayed for their cytotoxic activity against Hep G2 human liver cancer cell lines and BGC-823 human gastric cancer cell lines. Three of the 11 showed relatively dominant cytotoxicity against these cell lines. Conclusions A total of 12 known compounds have been identified from Panax japonicus C. A. Mey.(Bai San Qi,白三七);NMR spectra of compounds with similar skeletons showed regular characteristics;3 compounds showed relatively dominant cytotoxicity against Hep G2 and BGC-823 cancer cell lines, and the result can be valued as weak while setting the taxol as a positive control.展开更多
AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma ...AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.展开更多
The role of second messenger pathways, cyclic AMP, calcium,and protein and protein kinase C(PKC) in the transcriptional regulation of c-fos proto-oncogene expression in rat Sertoli cells was investigated, c-fos expre...The role of second messenger pathways, cyclic AMP, calcium,and protein and protein kinase C(PKC) in the transcriptional regulation of c-fos proto-oncogene expression in rat Sertoli cells was investigated, c-fos expression wasmonitored by Northern blot analysis.Although the action of FSH on Sertolicells is considered to be mediated by cAMP, dibutyryl cAMP(db cAMP), apotent membrane permeable analog of cAMP,induced much less c-fos mRNA expression than FSH(<50%), suggesting that additional cAMPindependent mechanisms may mediate the effect of FSH on c-fos. Specificintracellular inhibitors of PKC decreased c-fos induction in response to FSHby more than 50%. Ionomycin, which increases intracellular free calciumconcentration, induced c-fos expression significantly. These datademonstrate that Sertoli cell c-fos mRNA expression in under multifactorial regulation by cAMP, calcium, and PKC.展开更多
AIM： To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.METHODS： The esophageal squamo...AIM： To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.METHODS： The esophageal squamous carcinoma cell line （TE-13） was treated with different concentrations of nicotine （100 μg/mL and 200 μg/mL） or 200 μg/mL nicotine plus 100 μmol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 （MMP-2） was analyzed by zymography and ELISA.RESULTS： Nicotine （100 μg/mL, 200 μg/mL） enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine （200 μ/mL） stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2. CONCLUSION： Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect ofnicotine-enhanced esophageal squamous carcinoma cell migration and invasion.展开更多
This paper mainly explores the Australian Aboriginal-white relationship in two novels： The Secret River （2005） by non-Indigenous writer Kate Grenville, and Carpentaria （2006） by Indigenous novelist Alexis Wright,...This paper mainly explores the Australian Aboriginal-white relationship in two novels： The Secret River （2005） by non-Indigenous writer Kate Grenville, and Carpentaria （2006） by Indigenous novelist Alexis Wright, and compares the discursive strategies and narrative devices the authors have adopted to represent whiteness and Indigeneity, one from the European settlers＇ point of view, the other from the standpoint of an Aboriginal author. In The Secret River, Grenville resorts to the genre of historical novel as a way of reconciling the past. Though the novel challenges the racialised stereotypes of the Aboriginal people by adopting a double perspective （a reconfigured white perspective to refute the colonists＇ views）, the moral ambiguity of the settler identity is still complicit with the colonial discourse. On the other hand, Carpentaria rejects a narrow, essentialist categorization of the Aboriginal people and defamiliarises the concept of whiteness by foregrounding it in a critique rather than as the default norm. Set in a narrative related to the oral tradition, the novel brings Aboriginal cosmology into full play A comparison of the two novels provides a panoramic view of how the Aboriginal-white relationships are presented through the literary imaginary in Australia.展开更多
基金the funding support from the National Natural Science Foundation of China (No. 81703819 and No. 81874369)Hunan Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine (No. 2017-04)+1 种基金Hunan Provincial Key Laboratory of Dong Medicine (No. 2015TP1020-02)Students Research Innovative Program of Hunan Province (No. 2018413)
文摘Objective Tu Jia ethnomedicine is a unique medical system inherited and adhibited by Tu Jia minority living in central-south China. Panax japonicus C. A. Mey.(Bai San Qi,白三七) is recognized as an effective and rare medicinal plant to treat weakness, fatigue and rheumatism in Tu Jia ethnomedicine. This paper is to discover more substance evidence for the application of Tu Jia ethnomedicine. Methods Column chromatography and preparative high performance liquid chromatography (HPLC) was applied for isolation and purification;1H-NMR, 13C-NMR, 1H-1H COSY, HSQC and HMBC NMR spectra were applied for structure identification;Methyl thiazolyl tetrazolim (MTT) assays were applied for cytotoxicity evaluation. Results Totally 12 known compounds were isolated by column chromatography and preparative HPLC from rhizomes of Panax japonicus C. A. Mey.(Bai San Qi,白三七). Structures of these compounds were identified by their NMR spectra. All the 12 compounds were triterpenoid saponins. Five of them were oleanolic acid type, and the remaining 7 were dammarane type. Eleven compounds were assayed for their cytotoxic activity against Hep G2 human liver cancer cell lines and BGC-823 human gastric cancer cell lines. Three of the 11 showed relatively dominant cytotoxicity against these cell lines. Conclusions A total of 12 known compounds have been identified from Panax japonicus C. A. Mey.(Bai San Qi,白三七);NMR spectra of compounds with similar skeletons showed regular characteristics;3 compounds showed relatively dominant cytotoxicity against Hep G2 and BGC-823 cancer cell lines, and the result can be valued as weak while setting the taxol as a positive control.
基金Supported by The National Natural Science Foundation of China, No. 30972898
文摘AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.
文摘The role of second messenger pathways, cyclic AMP, calcium,and protein and protein kinase C(PKC) in the transcriptional regulation of c-fos proto-oncogene expression in rat Sertoli cells was investigated, c-fos expression wasmonitored by Northern blot analysis.Although the action of FSH on Sertolicells is considered to be mediated by cAMP, dibutyryl cAMP(db cAMP), apotent membrane permeable analog of cAMP,induced much less c-fos mRNA expression than FSH(<50%), suggesting that additional cAMPindependent mechanisms may mediate the effect of FSH on c-fos. Specificintracellular inhibitors of PKC decreased c-fos induction in response to FSHby more than 50%. Ionomycin, which increases intracellular free calciumconcentration, induced c-fos expression significantly. These datademonstrate that Sertoli cell c-fos mRNA expression in under multifactorial regulation by cAMP, calcium, and PKC.
基金Supported by Beijing Municipal Commission of Education, Science and Technology Program, No. KM200610025029Beijing Municipal Natural Science Foundation, No. 7072022
文摘AIM： To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.METHODS： The esophageal squamous carcinoma cell line （TE-13） was treated with different concentrations of nicotine （100 μg/mL and 200 μg/mL） or 200 μg/mL nicotine plus 100 μmol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 （MMP-2） was analyzed by zymography and ELISA.RESULTS： Nicotine （100 μg/mL, 200 μg/mL） enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine （200 μ/mL） stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2. CONCLUSION： Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect ofnicotine-enhanced esophageal squamous carcinoma cell migration and invasion.
文摘This paper mainly explores the Australian Aboriginal-white relationship in two novels： The Secret River （2005） by non-Indigenous writer Kate Grenville, and Carpentaria （2006） by Indigenous novelist Alexis Wright, and compares the discursive strategies and narrative devices the authors have adopted to represent whiteness and Indigeneity, one from the European settlers＇ point of view, the other from the standpoint of an Aboriginal author. In The Secret River, Grenville resorts to the genre of historical novel as a way of reconciling the past. Though the novel challenges the racialised stereotypes of the Aboriginal people by adopting a double perspective （a reconfigured white perspective to refute the colonists＇ views）, the moral ambiguity of the settler identity is still complicit with the colonial discourse. On the other hand, Carpentaria rejects a narrow, essentialist categorization of the Aboriginal people and defamiliarises the concept of whiteness by foregrounding it in a critique rather than as the default norm. Set in a narrative related to the oral tradition, the novel brings Aboriginal cosmology into full play A comparison of the two novels provides a panoramic view of how the Aboriginal-white relationships are presented through the literary imaginary in Australia.
