采用田间试验以基肥和返青肥两种施肥方式,施生物硅肥量为0、150、300、450、600、750 kg hm-2六个水平,研究了白浆型水稻土不同生物硅肥施用量对水稻生理指标的影响。结果表明,适宜的生物硅肥施用量(450 kg hm-2)对水稻叶片叶绿素含量...采用田间试验以基肥和返青肥两种施肥方式,施生物硅肥量为0、150、300、450、600、750 kg hm-2六个水平,研究了白浆型水稻土不同生物硅肥施用量对水稻生理指标的影响。结果表明,适宜的生物硅肥施用量(450 kg hm-2)对水稻叶片叶绿素含量有显著的影响,可使叶绿素的含量提高9.6%,从而延缓了水稻功能叶片的光合功能期;水稻叶片的保护酶(CAT、POD)在施用生物硅肥后可维持较高的活性或受到抑制程度较低,使所受膜脂过氧化程度(MDA的含量)相对较低,生物硅肥施用量为450 kg hm-2时水稻叶片的CAT、POD活性达最大值,MDA的含量达到最低值,这说明施用生物硅肥促进了膜脂脱氧化作用,延缓功能叶片的衰老;施用生物硅肥后提高了植株干物质的合成与积累,促进了可溶性糖向淀粉的转移和碳水化合物的运输,协调了源、库关系。在本试验中,生物硅肥施用量为450 kg hm-2效果最好,施肥效果各处理表现为:Si3>Si4>Si5>Si2>Si1>Si0,各处理间差异达到极显著,两种施肥方式做基肥的要比返青肥的效果好。展开更多
A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope t...A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging.Pooled plasma from different liver fibrosis stages,which were assessed in advance by the current gold-standard of liver biopsy,was quantitatively analyzed.A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process,and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis.Validation results of fibronectin by Western blotting reconfirmed the mass-based data.Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients.Consequently,quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis.We quantitatively monitored the fibrogenesis process in CHB patients.We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease.These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.展开更多
文摘采用田间试验以基肥和返青肥两种施肥方式,施生物硅肥量为0、150、300、450、600、750 kg hm-2六个水平,研究了白浆型水稻土不同生物硅肥施用量对水稻生理指标的影响。结果表明,适宜的生物硅肥施用量(450 kg hm-2)对水稻叶片叶绿素含量有显著的影响,可使叶绿素的含量提高9.6%,从而延缓了水稻功能叶片的光合功能期;水稻叶片的保护酶(CAT、POD)在施用生物硅肥后可维持较高的活性或受到抑制程度较低,使所受膜脂过氧化程度(MDA的含量)相对较低,生物硅肥施用量为450 kg hm-2时水稻叶片的CAT、POD活性达最大值,MDA的含量达到最低值,这说明施用生物硅肥促进了膜脂脱氧化作用,延缓功能叶片的衰老;施用生物硅肥后提高了植株干物质的合成与积累,促进了可溶性糖向淀粉的转移和碳水化合物的运输,协调了源、库关系。在本试验中,生物硅肥施用量为450 kg hm-2效果最好,施肥效果各处理表现为:Si3>Si4>Si5>Si2>Si1>Si0,各处理间差异达到极显著,两种施肥方式做基肥的要比返青肥的效果好。
基金supported by the National Basic Research Program of China(Grant Nos.2011CB910601,2011CB910700,2010CB912700 and 2011CB505304)the National High Technology Research and Development Program of China (Grant No.2006AA02A308)+4 种基金National Natural Science Foundation of China(Grant Nos.30700356,30700988,30972909,81000192,81001470 and 81010064)Chinese State Key Project Specialized for Infectious Diseases (Grant Nos.2008ZX10002-016 and 2009ZX10004-103)the National Key Technologies R&D Program for New Drugs (Grant No.2009ZX09301-002)the International Scientific Collaboration Program (Grant Nos.2009DFB33070 and 2010DFA31260)State Key Laboratory of Proteomics(Grant Nos.SKLP-Y200901 and SKLP-O200901)
文摘A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging.Pooled plasma from different liver fibrosis stages,which were assessed in advance by the current gold-standard of liver biopsy,was quantitatively analyzed.A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process,and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis.Validation results of fibronectin by Western blotting reconfirmed the mass-based data.Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients.Consequently,quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis.We quantitatively monitored the fibrogenesis process in CHB patients.We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease.These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.
