母乳中的白细胞介素-10(IL-10)可能对新生儿有保护作用,使他们免受肠道炎症综合症的折磨。而早产和未经母乳喂养的婴儿则易于患此病。University of Texas Medical Branch(UTMB)(Galveston,TX)的研究者不仅在生育后不久、哺乳母亲的乳...母乳中的白细胞介素-10(IL-10)可能对新生儿有保护作用,使他们免受肠道炎症综合症的折磨。而早产和未经母乳喂养的婴儿则易于患此病。University of Texas Medical Branch(UTMB)(Galveston,TX)的研究者不仅在生育后不久、哺乳母亲的乳汁中发现了IL-10,而且他们还认为,乳汁中的细胞以某种方式包裹这种细胞因子,因此这种因子能到达婴儿的肠道而不被消化。 UTMB的Roberto Garofalo说,在取自生育后不到80小时的20位新母亲的乳汁样品中,均含有可检测浓度的IL-10。IL-10以灭活细胞的方式缓解炎症,这些细胞诱导IL-10,包括:单核细胞。展开更多
Abstract:Objective To assess whether interleukin-10 (IL-10) is chondroprotective in vitro. Methods Chondrocytes were isolated from femoral cartilage of rats (7-10 days) by digestion with collagenase Ⅱ. The first pass...Abstract:Objective To assess whether interleukin-10 (IL-10) is chondroprotective in vitro. Methods Chondrocytes were isolated from femoral cartilage of rats (7-10 days) by digestion with collagenase Ⅱ. The first passage cells were grown in 24- well plates with DMEM, supplemented with 10% fetal bovine serum, for 2-4 days. The cells were then cultured in 0.1% fetal bovine serum DMEM medium, and given respectively interleukin-1 (IL-1) 100?μ/ml, IL-1 100?μ/ml+recombinant murine interleukin-10 (rmIL-10) 20?ng/ml, rmIL-10 20?ng/ml, and cultured for 48 hours. Scanning electron morphology and immunohistochemical study of nitric oxide synthase 2 and matric metalloproteinase 3 mRNA in situ hybridization were performed. Cell proliferation and morphology were observed under inverted microscope from the beginning of cell culture for three weeks. Results IL-1 stimulated granule production in the cytoplasma of chondrocytes, and the cells died in the second and third weeks of culture. IL-10 antagonized IL-1, protected the cells from death and maintained chondrocyte proliferation. Scanning electron morphology showed that IL-1 stimulated the formation of numerous microvilli on the cell surface, while thin and less numerous microvilli were found in cultures with IL-10. Immunohistochemical study and in situ hybridization showed that IL-10 inhibited NOS2 and MMP3 expression.Conclusion IL-10 not only inhibits the synthesis of inflammatory cytokines, but also directly protects chondrocytes by antagonizing IL-1.展开更多
文摘母乳中的白细胞介素-10(IL-10)可能对新生儿有保护作用,使他们免受肠道炎症综合症的折磨。而早产和未经母乳喂养的婴儿则易于患此病。University of Texas Medical Branch(UTMB)(Galveston,TX)的研究者不仅在生育后不久、哺乳母亲的乳汁中发现了IL-10,而且他们还认为,乳汁中的细胞以某种方式包裹这种细胞因子,因此这种因子能到达婴儿的肠道而不被消化。 UTMB的Roberto Garofalo说,在取自生育后不到80小时的20位新母亲的乳汁样品中,均含有可检测浓度的IL-10。IL-10以灭活细胞的方式缓解炎症,这些细胞诱导IL-10,包括:单核细胞。
文摘Abstract:Objective To assess whether interleukin-10 (IL-10) is chondroprotective in vitro. Methods Chondrocytes were isolated from femoral cartilage of rats (7-10 days) by digestion with collagenase Ⅱ. The first passage cells were grown in 24- well plates with DMEM, supplemented with 10% fetal bovine serum, for 2-4 days. The cells were then cultured in 0.1% fetal bovine serum DMEM medium, and given respectively interleukin-1 (IL-1) 100?μ/ml, IL-1 100?μ/ml+recombinant murine interleukin-10 (rmIL-10) 20?ng/ml, rmIL-10 20?ng/ml, and cultured for 48 hours. Scanning electron morphology and immunohistochemical study of nitric oxide synthase 2 and matric metalloproteinase 3 mRNA in situ hybridization were performed. Cell proliferation and morphology were observed under inverted microscope from the beginning of cell culture for three weeks. Results IL-1 stimulated granule production in the cytoplasma of chondrocytes, and the cells died in the second and third weeks of culture. IL-10 antagonized IL-1, protected the cells from death and maintained chondrocyte proliferation. Scanning electron morphology showed that IL-1 stimulated the formation of numerous microvilli on the cell surface, while thin and less numerous microvilli were found in cultures with IL-10. Immunohistochemical study and in situ hybridization showed that IL-10 inhibited NOS2 and MMP3 expression.Conclusion IL-10 not only inhibits the synthesis of inflammatory cytokines, but also directly protects chondrocytes by antagonizing IL-1.
