Interleukin 1β has been reported to be involved in pain modulation in central n ervous systems. As the spinal cord is a major region in which nociceptive input is processed and modulated, we examined the effect of in...Interleukin 1β has been reported to be involved in pain modulation in central n ervous systems. As the spinal cord is a major region in which nociceptive input is processed and modulated, we examined the effect of intrathecal injection of i nterleukin 1β on the nociception in carrageenan injected rats using behavior a nd Fos study. The results showed that: ① intrathecally administered interleu kin 1β (1 ng,10 ng and 100 ng) could dose dependently increase the thermal n ociceptive threshold of carrageenan inflammed rats. Intrathecally administered interleukin 1β 10 ng could significantly increase the paw withdrawal latency , peaked at 15 min and lasted for more than 30 min. Electroacupuncture (EA) unilat eral (ipsilateral to carrageenan injection paw) "Zusanli"(ST 36) and "Kunlun "(BL 60) acupoints (2 and 60 Hz alternately,1～3 mA,30 min) significantly eleva ted the nociception threshold of the carrageenan injected paws. When 10 ng inte rleukin 1β was intrathecally injected 5 min before the beginning of EA, the ef fect of EA analgesia was markedly potentiated. ② The number of Fos LI neurons in the ipsilateral spinal dorsal horn was significantly increased in laminae Ⅰ ～Ⅱ and laminae Ⅴ～Ⅵ in carrageenan injected rats. Intrathecal administratio n of interleukin 1β could suppress the expression of Fos LI in laminae Ⅰ～Ⅱ , but not in laminae Ⅲ～Ⅳ or laminae Ⅴ～Ⅵ. The number of Fos LI positive n eurons both in laminae Ⅰ～Ⅱ and in laminae Ⅴ～Ⅵ in EA treated rats was sign ificantly less than that of vehicle treated rats. When 10 ng interleukin 1β wa s intrathecally injected 5 min before the beginning of EA, the number of Fos LI positive neurons both in laminae Ⅰ～Ⅱ and in laminae Ⅴ～Ⅵ was markedly le ss than either of those in EA treated rats or in interleukin 1β treated rats. The results suggest that interleukin 1β in spinal level could produce antinoci ceptive effect and strengthen acupuncture analgesia in inflammatory rat.展开更多
目的 探讨CD137通路参与老年血管慢性炎症的机制。方法 选择C57/B6小鼠40只,CD137小鼠20只,将小鼠分为对照组、衰老组、衰老CD137组,每组20只。通过Luminex液相芯片多因子检测技术检测各组血浆炎性因子表达。通过qPCR技术及免疫荧光技...目的 探讨CD137通路参与老年血管慢性炎症的机制。方法 选择C57/B6小鼠40只,CD137小鼠20只,将小鼠分为对照组、衰老组、衰老CD137组,每组20只。通过Luminex液相芯片多因子检测技术检测各组血浆炎性因子表达。通过qPCR技术及免疫荧光技术对各组血管中白细胞介素(IL)-1β进行定量和定位。分别从C57/B6和CD137^(-/-)小鼠主动脉中提取并原代培养血管平滑肌细胞(VSMC)。通过博来霉素体外诱导细胞衰老,采用Western blot、qPCR分别检测各组细胞核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、IL-βmRNA和蛋白表达,采用ELISA检测细胞分泌IL-1β水平。结果 对照组、衰老组、衰老CD137组血浆IL-1β水平分别为(10.86±0.89)pg/ml、(61.40±3.88)pg/ml和(18.20±1.25)pg/ml。衰老组血浆趋化因子配体5、IL-3、IL-13、TNF-α、IL-1β水平较对照组明显升高,衰老CD137^(-/-)组血浆趋化因子配体5、IL-3、IL-13、TNF-α、IL-1β水平较衰老组明显降低(P<0.01)。衰老组血管NLRP3和IL-1βmRNA表达较对照组和衰老CD137组明显升高(3.45±0.62 vs 1.00±0.00,1.57±0.17,P<0.05;5.89±0.82 vs 1.00±0.00,2.64±0.34,P<0.05)。体外实验中,衰老组VSMC中NLRP3和IL-1β蛋白表达较对照组和衰老CD137^(-/-)组明显升高(1.65±0.05 vs 1.00±0.00,0.85±0.05,P<0.05;1.45±0.05 vs 1.00±0.00,1.35±0.05,P<0.05)。衰老组VSMC上清中IL-1β水平较对照组和衰老CD137^(-/-)组明显升高(27.50±4.32 vs 14.20±3.52,16.50±2.72,P<0.05)。结论 CD137通过调控VSMC中NLRP3、IL-1β表达参与老年血管慢性炎症。展开更多
文摘Interleukin 1β has been reported to be involved in pain modulation in central n ervous systems. As the spinal cord is a major region in which nociceptive input is processed and modulated, we examined the effect of intrathecal injection of i nterleukin 1β on the nociception in carrageenan injected rats using behavior a nd Fos study. The results showed that: ① intrathecally administered interleu kin 1β (1 ng,10 ng and 100 ng) could dose dependently increase the thermal n ociceptive threshold of carrageenan inflammed rats. Intrathecally administered interleukin 1β 10 ng could significantly increase the paw withdrawal latency , peaked at 15 min and lasted for more than 30 min. Electroacupuncture (EA) unilat eral (ipsilateral to carrageenan injection paw) "Zusanli"(ST 36) and "Kunlun "(BL 60) acupoints (2 and 60 Hz alternately,1～3 mA,30 min) significantly eleva ted the nociception threshold of the carrageenan injected paws. When 10 ng inte rleukin 1β was intrathecally injected 5 min before the beginning of EA, the ef fect of EA analgesia was markedly potentiated. ② The number of Fos LI neurons in the ipsilateral spinal dorsal horn was significantly increased in laminae Ⅰ ～Ⅱ and laminae Ⅴ～Ⅵ in carrageenan injected rats. Intrathecal administratio n of interleukin 1β could suppress the expression of Fos LI in laminae Ⅰ～Ⅱ , but not in laminae Ⅲ～Ⅳ or laminae Ⅴ～Ⅵ. The number of Fos LI positive n eurons both in laminae Ⅰ～Ⅱ and in laminae Ⅴ～Ⅵ in EA treated rats was sign ificantly less than that of vehicle treated rats. When 10 ng interleukin 1β wa s intrathecally injected 5 min before the beginning of EA, the number of Fos LI positive neurons both in laminae Ⅰ～Ⅱ and in laminae Ⅴ～Ⅵ was markedly le ss than either of those in EA treated rats or in interleukin 1β treated rats. The results suggest that interleukin 1β in spinal level could produce antinoci ceptive effect and strengthen acupuncture analgesia in inflammatory rat.
文摘目的 探讨CD137通路参与老年血管慢性炎症的机制。方法 选择C57/B6小鼠40只,CD137小鼠20只,将小鼠分为对照组、衰老组、衰老CD137组,每组20只。通过Luminex液相芯片多因子检测技术检测各组血浆炎性因子表达。通过qPCR技术及免疫荧光技术对各组血管中白细胞介素(IL)-1β进行定量和定位。分别从C57/B6和CD137^(-/-)小鼠主动脉中提取并原代培养血管平滑肌细胞(VSMC)。通过博来霉素体外诱导细胞衰老,采用Western blot、qPCR分别检测各组细胞核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、IL-βmRNA和蛋白表达,采用ELISA检测细胞分泌IL-1β水平。结果 对照组、衰老组、衰老CD137组血浆IL-1β水平分别为(10.86±0.89)pg/ml、(61.40±3.88)pg/ml和(18.20±1.25)pg/ml。衰老组血浆趋化因子配体5、IL-3、IL-13、TNF-α、IL-1β水平较对照组明显升高,衰老CD137^(-/-)组血浆趋化因子配体5、IL-3、IL-13、TNF-α、IL-1β水平较衰老组明显降低(P<0.01)。衰老组血管NLRP3和IL-1βmRNA表达较对照组和衰老CD137组明显升高(3.45±0.62 vs 1.00±0.00,1.57±0.17,P<0.05;5.89±0.82 vs 1.00±0.00,2.64±0.34,P<0.05)。体外实验中,衰老组VSMC中NLRP3和IL-1β蛋白表达较对照组和衰老CD137^(-/-)组明显升高(1.65±0.05 vs 1.00±0.00,0.85±0.05,P<0.05;1.45±0.05 vs 1.00±0.00,1.35±0.05,P<0.05)。衰老组VSMC上清中IL-1β水平较对照组和衰老CD137^(-/-)组明显升高(27.50±4.32 vs 14.20±3.52,16.50±2.72,P<0.05)。结论 CD137通过调控VSMC中NLRP3、IL-1β表达参与老年血管慢性炎症。
