犬心脏移植围术期血清IL-6、IL-8、TNF-α的变化

目的探讨犬心脏移植手术中血清白细胞介质6(interleukin-6,IL-6)、白细胞介质8(interleukin-8,IL-8)、肿瘤坏死因子(αtumor necrosis factor-α,TNF-α)浓度的变化。方法20只家犬随机分组配对进行心脏移植手术,分别在麻醉后、转机时、停机时、手术结束时取动脉血用酶联免疫吸附法(ELISA)检测受体犬血清中的IL-6I、L-8、TNF-α浓度。结果犬血清IL-6I、L-8、TNF-α浓度在麻醉后持续上升,以停机时和手术结束时更为明显(P<0.05)。结论犬心脏移植手术中存在明显的应激反应和缺血再灌注损伤。

乌司他丁在犬心脏移植手术中对血清IL-6、IL-8、TNF-α影响的观察

目的探讨犬心脏移植手术中乌司他丁(ulinastatin)对白细胞介质6(interleukin-6,IL-6)、白细胞介质8(interleukin-8,IL-8)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的影响。方法 32只家犬随机配对分成对照组(C组)和乌司他丁组(U组)进行心脏移植手术,分别在麻醉后(T1)、转机时(T2)、停机时(T3)、手术结束时(T4),取动脉血用酶联免疫吸附法(ELISA)检测受体犬血清中的IL-6、IL-8、TNF-α。结果 U组的IL-6和IL-8从T2开始显著小于C组,U组的TNF-α从T3开始显著小于C组(P<0.05)。结论乌司他丁可以减轻心脏移植手术中的炎症反应。

Expression and cellular localization of interleukin 8 mRNA and protein in the area of xenogenic bone implant

Objective: To observe the expression and cellular localization of interleukin 8 (IL 8) mRNA and protein in the area of xenogenic bone implant. Methods: The bovine cancellous bone granules were implanted into the thigh muscles of mice. The samples were taken 4, 7, 14 and 21 days after implantation. IL 8mRNA and protein in the site of implant were assayed by in situ hybridization and immunocytochemical techniques. Results: The expression of IL 8mRNA and protein were observed in all specimens 4, 7, 14 and 21 days after implantation. IL 8mRNA was expressed mainly by the neutrophils, monocytes, macrophages and fibroblasts at 7th day post implantation. Some mesenchymal cells, multinucleated giant cells, vascular endothelial cells and smooth muscle cells also expressed IL 8mRNA in the area of xenogenic bone implant at 14th and 21st days. Immunocytochemical studies demonstrated the same results as that of in situ hybridization. Conclusions: Many different kinds of cells express IL 8mRNA and secret IL 8 in the area of xenogenic bone implant, suggesting that IL 8 may play an important role in local immunity of xenogenic bone graft.