Objective:To conduct a prospective cohort study to clarify the relationship between human leukocyte antigen (HLA) polymorphisms and the seroconversion of hepatitis B e antigen(HBeAg). Study design:In the prospective c...Objective:To conduct a prospective cohort study to clarify the relationship between human leukocyte antigen (HLA) polymorphisms and the seroconversion of hepatitis B e antigen(HBeAg). Study design:In the prospective cohort study,81 HBeAg-positive children with chronic hepatitis B virus(HBV) infection from 40 unrelated families were recruited and followed-up regularly for a mean period of 17.70 ±3.23years. The association between HLA antigen and the age at HBeAg seroconversion was analyzed using Cox regression model with shared frailties under left truncation and right censorship. Results:HLA-B61 and HLA-DQB1*0503 antigen spredicted a higher HBeAg seroconversion rate (relative incidence = 6.17 and 3.22,P = 0.024 and 0.017,respectively).Within-family frailty in our sibling cohort study demonstrated a negligible or a low degree of within-family correlation with spontaneous HBeAg seroconversion in each HLA antigen. Conclusions:HLA class I antigen B61 and class II antigen DQB1*0503 are associated with earlier HBeAg seroconversion in Taiwan Residents children with chronic HBV infection.展开更多
目的探讨基于测序的人类白细胞抗原分型（HLA—sequencing—basedtyping，HLA-SBT）和PCR短串联重复序列（short tandem repeat，STR）技术在人胚胎干细胞（human embryonic stem cell，hESC）应用前检测中的运用，建立人胚胎干细胞系...目的探讨基于测序的人类白细胞抗原分型（HLA—sequencing—basedtyping，HLA-SBT）和PCR短串联重复序列（short tandem repeat，STR）技术在人胚胎干细胞（human embryonic stem cell，hESC）应用前检测中的运用，建立人胚胎干细胞系的基因型档案。方法人胚胎干细胞系SYSU-1、SYSU-3，分别培养到20代、40代，应用PCR寡核苷酸特异测序探针（sequence specific olignucleotide probe，SSO）技术检测两株细胞系的HLA-A、-B、-DR位点的低分辨分型，再利用HLA—SBT技术检测两株细胞系的HLA-A、-B、-DR位点的高分辨分型。应用PCR—STR技术检测两株细胞系的基因遗传标记。结果获得两株hESC细胞系的HLA高分辨分型和STR基因型。结论可以运用HLA—SBT和PCR．STR技术建立人胚胎干细胞应用前的基因型档案。展开更多
目的评价巢式聚合酶链反应．序列特异引物法（polymerase chain reaction-sequence specific primer，PCR-SSP）用于人类种植前胚胎的单个卵裂球HLA-A位点分型的准确性和可靠性。方法通过扩增HLA-A第2外显子评价单卵裂球巢式第1轮扩增...目的评价巢式聚合酶链反应．序列特异引物法（polymerase chain reaction-sequence specific primer，PCR-SSP）用于人类种植前胚胎的单个卵裂球HLA-A位点分型的准确性和可靠性。方法通过扩增HLA-A第2外显子评价单卵裂球巢式第1轮扩增的成功率，用巢式PCR-SSP法进行单卵裂球HLA-A分型。结果从10个家系的体外受精剩余胚胎中获得120个卵裂球，巢式第1轮扩增平均成功率为86．7％，其中前期家系F1-F5为78．2％，后期家系F6-F10为93．8％；对80个卵裂球进一步做SSP分型，其中11个卵裂球HLA-A第2外显子扩增失败，无分型结果，而69个卵裂球HLA-A第2外显子扩增成功，都获得分型结果。除6个卵裂球因亲本纯合无法判断是否发生缺失外，剩下63个卵裂球中，基因型与根据亲本预测的结果相符的有59个，占93．6％；显示缺少部分亲本等位基因的有3个，占4．8％；显示部分亲本等位基因重复的1个，占1．6％。结论在第1轮扩增成功的基础上，巢式PCR-SSP法用于单卵裂球HLA-A分型准确可靠，可用于临床上筛选与需要造血干细胞移植的患儿HLA型等同的胚胎。展开更多
文摘Objective:To conduct a prospective cohort study to clarify the relationship between human leukocyte antigen (HLA) polymorphisms and the seroconversion of hepatitis B e antigen(HBeAg). Study design:In the prospective cohort study,81 HBeAg-positive children with chronic hepatitis B virus(HBV) infection from 40 unrelated families were recruited and followed-up regularly for a mean period of 17.70 ±3.23years. The association between HLA antigen and the age at HBeAg seroconversion was analyzed using Cox regression model with shared frailties under left truncation and right censorship. Results:HLA-B61 and HLA-DQB1*0503 antigen spredicted a higher HBeAg seroconversion rate (relative incidence = 6.17 and 3.22,P = 0.024 and 0.017,respectively).Within-family frailty in our sibling cohort study demonstrated a negligible or a low degree of within-family correlation with spontaneous HBeAg seroconversion in each HLA antigen. Conclusions:HLA class I antigen B61 and class II antigen DQB1*0503 are associated with earlier HBeAg seroconversion in Taiwan Residents children with chronic HBV infection.
文摘目的探讨基于测序的人类白细胞抗原分型（HLA—sequencing—basedtyping，HLA-SBT）和PCR短串联重复序列（short tandem repeat，STR）技术在人胚胎干细胞（human embryonic stem cell，hESC）应用前检测中的运用，建立人胚胎干细胞系的基因型档案。方法人胚胎干细胞系SYSU-1、SYSU-3，分别培养到20代、40代，应用PCR寡核苷酸特异测序探针（sequence specific olignucleotide probe，SSO）技术检测两株细胞系的HLA-A、-B、-DR位点的低分辨分型，再利用HLA—SBT技术检测两株细胞系的HLA-A、-B、-DR位点的高分辨分型。应用PCR—STR技术检测两株细胞系的基因遗传标记。结果获得两株hESC细胞系的HLA高分辨分型和STR基因型。结论可以运用HLA—SBT和PCR．STR技术建立人胚胎干细胞应用前的基因型档案。
文摘目的评价巢式聚合酶链反应．序列特异引物法（polymerase chain reaction-sequence specific primer，PCR-SSP）用于人类种植前胚胎的单个卵裂球HLA-A位点分型的准确性和可靠性。方法通过扩增HLA-A第2外显子评价单卵裂球巢式第1轮扩增的成功率，用巢式PCR-SSP法进行单卵裂球HLA-A分型。结果从10个家系的体外受精剩余胚胎中获得120个卵裂球，巢式第1轮扩增平均成功率为86．7％，其中前期家系F1-F5为78．2％，后期家系F6-F10为93．8％；对80个卵裂球进一步做SSP分型，其中11个卵裂球HLA-A第2外显子扩增失败，无分型结果，而69个卵裂球HLA-A第2外显子扩增成功，都获得分型结果。除6个卵裂球因亲本纯合无法判断是否发生缺失外，剩下63个卵裂球中，基因型与根据亲本预测的结果相符的有59个，占93．6％；显示缺少部分亲本等位基因的有3个，占4．8％；显示部分亲本等位基因重复的1个，占1．6％。结论在第1轮扩增成功的基础上，巢式PCR-SSP法用于单卵裂球HLA-A分型准确可靠，可用于临床上筛选与需要造血干细胞移植的患儿HLA型等同的胚胎。
