The genus Leucogyrophana Pouz.was found from Yunnan Province,southwest China,and it was the first report from China.Accordingly,Leuogyrophana pseudomollusa(Parm.)Parm.was the first record of the Chinese fungal flora.L...The genus Leucogyrophana Pouz.was found from Yunnan Province,southwest China,and it was the first report from China.Accordingly,Leuogyrophana pseudomollusa(Parm.)Parm.was the first record of the Chinese fungal flora.L.pseudomollusa grew on rotten wood of Pinus.The present paper provided a detailed description and illustration based on the Chinese material.展开更多
用磁珠-生物素标记的微卫星探针(ACA)15与白缘缺(Leiobagrus marginatus)基因组酶切片段杂交,捕获含有微卫星序列的DNA片段,连接到T载体中,构建富集微卫星序列的小片段插入文库,再用γ-32P标记的探针进行二次筛选,获得阳性克隆785个,对...用磁珠-生物素标记的微卫星探针(ACA)15与白缘缺(Leiobagrus marginatus)基因组酶切片段杂交,捕获含有微卫星序列的DNA片段,连接到T载体中,构建富集微卫星序列的小片段插入文库,再用γ-32P标记的探针进行二次筛选,获得阳性克隆785个,对其中的140个克隆进行了测序,获得完整的微卫星序列132个．用引物设计软件Primer Premier 5．0设计引物64对．对其中的20对引物进行了多态性检测,筛选到稳定扩增的标记13对,用此13对微卫星引物分析了缺属白缘缺(L．marginalus)、拟缘缺(L．marginatoides)(大、小各1种)、黑尾缺(L．nigricauda)共4个群体的遗传多样性,其中10对微卫星引物在种内或种间表现出多态性．展开更多
A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymus racemosus Lam., Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C. ...A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymus racemosus Lam., Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C. Koch respectively was made. In the course of germplasm development, genome analysis by means of chromosome banding, genomic in situ hybridization (GISH) or fluorescence in situ hybridization (FISH), molecular markers, particularly restriction fragment length polymorphism (RFLP) coupled with aneuploid analysis was employed for the purpose of improving breeding efficiency. Potential use of such germplasm in wheat breeding practice, basic studies and some related problems were also discussed.展开更多
文摘The genus Leucogyrophana Pouz.was found from Yunnan Province,southwest China,and it was the first report from China.Accordingly,Leuogyrophana pseudomollusa(Parm.)Parm.was the first record of the Chinese fungal flora.L.pseudomollusa grew on rotten wood of Pinus.The present paper provided a detailed description and illustration based on the Chinese material.
文摘用磁珠-生物素标记的微卫星探针(ACA)15与白缘缺(Leiobagrus marginatus)基因组酶切片段杂交,捕获含有微卫星序列的DNA片段,连接到T载体中,构建富集微卫星序列的小片段插入文库,再用γ-32P标记的探针进行二次筛选,获得阳性克隆785个,对其中的140个克隆进行了测序,获得完整的微卫星序列132个．用引物设计软件Primer Premier 5．0设计引物64对．对其中的20对引物进行了多态性检测,筛选到稳定扩增的标记13对,用此13对微卫星引物分析了缺属白缘缺(L．marginalus)、拟缘缺(L．marginatoides)(大、小各1种)、黑尾缺(L．nigricauda)共4个群体的遗传多样性,其中10对微卫星引物在种内或种间表现出多态性．
文摘A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymus racemosus Lam., Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C. Koch respectively was made. In the course of germplasm development, genome analysis by means of chromosome banding, genomic in situ hybridization (GISH) or fluorescence in situ hybridization (FISH), molecular markers, particularly restriction fragment length polymorphism (RFLP) coupled with aneuploid analysis was employed for the purpose of improving breeding efficiency. Potential use of such germplasm in wheat breeding practice, basic studies and some related problems were also discussed.
