牛磺酸和/或递增负荷训练对大鼠骨骼肌不同肌纤维线粒体Ca^(2+)-ATP酶活性的影响

以大鼠力竭性运动为模型 ,观察了牛磺酸和 /或递增负荷训练对大鼠力竭运动后即刻红、白肌线粒体Ca2 + ATP酶活性的影响。结果显示 :1)安静时白肌纤维线粒体Ca2 + ATP酶活性较红肌的高 ;2 )力竭运动使白肌纤维线粒体Ca2 + ATP酶活性降低 ,红肌的则升高 ;3) 5周递增负荷训练对白肌纤维线粒体Ca2 + ATP酶活性影响较大 ,而对红肌的几乎没有什么影响 ;4)牛磺酸能使力竭运动后白肌和红肌张维线粒体Ca2 + ATP酶活性升高 ,结合训练其作用更明显。结果提示 :递增负荷训练中慢运动单位可能较少参与运动 ,所以该方法可能只对白肌起到积极的锻炼作用 ,牛磺酸和

Identification of Myosinon the Surface of Wheat Mitochondria

Itwasdemonstratedthatmyosinwasassociatedwiththesurfaceofmitochondriainwheat (Triticum aestivumL .) .AssaysofpolyacrylamidegelelectrophoresisandWesternblottinghaveshownthatapolypeptide withmolecularweightof2 10kDcouldberecognizedbyapolyclonalantibodyagainsthumanmusclemyosin .It wasfoundthattheATPaseactivityofmitochondrialsuspensioncouldbestimulatedbyF_actinisolatedfrom chickenmuscle ,whichindicatedthattherewasmyosinonthesurfaceofwheatmitochondria .Thisresultwas confirmedbyelectronmicroscopicobservation :mitochondriatreatedwithN_ethylmaleimide (NEM)couldbe wrappedbytheF_actin .