脂蛋白脂肪酶基因及载脂蛋白酶E基因突变与妊娠期高血压疾病关系的研究进展

妊娠期高血压疾病（HDCP）是妊娠期出现的特发性高血压综合征，一般出现于妊娠晚期，HDCP多发生于妊娠20周以后，临床表现为高血压，尿蛋白，水肿，严重时出现抽搐，昏迷，甚至母婴死亡，为孕产妇及围生儿死亡的重要死因，其发病率高达7％～10％。

脂蛋白脂肪酶基因突变（Glu242-Lys）伴发糖尿病一例

患者女，38岁。因反复发作上腹痛22年，口干，多饮、多尿1年收入院。患者于22年前出现无明显诱因的上腹痛，查血、尿淀粉酶明显升高，诊断为急性胰腺炎，经治疗后好转，其后每年反复发作1～6次。近1年有口干、多饮、多尿，曾测空腹血糖8．0mmol／L，未治疗。入院前1天再次发生上腹痛，无恶心、呕吐，无腹泻。既往有高血脂病史22年，

白假丝酵母脂肪酶CaLIP5在毕赤酵母X33中的优化重组表达

[目的]研究新的脂肪酶及优化脂肪酶发酵培养条件。[方法]在毕赤酵母X33系统中重组表达白假丝酵母脂肪酶CaLIP5。利用响应面分析法,构建了CaLIP5的酶活回归方程。[结果]由响应面分析可知,在温度25.71℃,pH8.33,酵母提取物浓度1.28%和葡萄糖浓度4.06%时,预测最大酶活为8.33U/ml。试验验证酶活为9.24U/ml。实际试验值与模型预测值基本一致。优化后的酶活提高了22.22%。[结论]在优化的培养条件下,可以获得毕赤酵母X33重组表达CaLIP5的最大酶活。

《饲料脂肪水平对南美白对虾生长及营养机能的影响》获第三届广西青年学术年会征文二等奖

由广西壮族自治区科学技术协会、广西壮族自治区社会科学联合会和共青团广西壮族自治区委员会联合组织召开的“第三届广西青年学术年会”，共征集学术论文558篇，围绕年会的主题“广西青年学者的历史责任——实现富民兴桂新跨越”开展了全方位、

血清游离脂肪酸/白蛋白比值联合血钙在急性胰腺炎中的临床价值研究

目的本研究旨在探讨游离脂肪酸/白蛋白比值联合血钙在急性胰腺炎中的临床价值。方法回顾性分析2015年1月至2018年11月就诊于陕西省咸阳市中心医院的急性胰腺炎患者36例,在电子病历系统收集急性胰腺炎患者的临床资料,并检测患者的游离脂肪酸、白蛋白、血钙水平,并计算出游离脂肪酸白蛋白比值。通过对比患者的游离脂肪酸白蛋白比值和急性胰腺炎患者的血钙下降程度在预测疾病的预后中的价值,从而获得游离脂肪酸白蛋白比值联合血钙在预测急性胰腺炎患者转归中的临床价值。结果轻症急性胰腺炎患者的游离脂肪酸浓度、重度急性胰腺炎患者的游离脂肪酸浓度、轻症急性胰腺炎患者游离脂肪酸/白蛋白比值。重度急性胰腺炎患者游离脂肪酸/白蛋白比值重度急性胰腺炎患者白蛋白水平明显低于轻症急性胰腺炎患者白蛋白水平,重度急性胰腺炎患者血钙水平明显低于轻症急性胰腺炎患者血钙水平,两组间游离脂肪酸、白蛋白、钙及游离脂肪酸/白蛋白比值差异有显著性(P<0.05)。同时随着重度急性胰腺炎严重程度的增加游离脂肪酸/白蛋白比值逐渐上升,血钙水平逐渐下降。结论联合测定游离脂肪酸/白蛋白比值、血钙对判断急性胰腺炎的严重程度有良好的价值,且游离脂肪酸/白蛋白比值可能成为判断急性胰腺炎轻重程度的一个独立因素。

米色脂肪细胞来源的研究进展

机体中存在两类脂肪细胞,白脂肪细胞和棕脂肪细胞。在寒冷和肾上腺素等的刺激下,白脂肪组织中出现一种与经典棕脂肪细胞显著不同的新型脂肪细胞——米色脂肪细胞,其形态、表型和基因表达均介于白脂肪细胞和棕脂肪细胞之间。但米色脂肪细胞与另两种脂肪细胞的发育起源不同,其起源具有多样性,且平滑肌细胞是其可能的前体细胞之一。棕色化过程中米色细胞的来源尚有争议,一部分学者认为其由前体细胞从头分化而来,而另一派学者支持它由白脂肪细胞转分化而来。米色脂肪细胞在治疗肥胖症和代谢综合征上有很好的应用前景。

肥胖者药物动力学变化

体内脂肪过多的积聚会使高血压、动脉硬化、冠心、糖尿病发病率增加,也使乳腺癌、结肠癌、前列腺癌、子宫内腺癌、卵巢癌等的发病率增加,因而一般来说肥胖者比非肥胖者更需要医学治疗,值得重视的是治疗药物的药物动力学与药效学在肥胖者和非肥胖者可能有所改变,例如药物的分布容积(Vd)和清除率(CL)的变比会影响到药物的作用,所以需明确肥胖者药物动力学性质的变化.

Genetic Screening of the Lipoprotein Lipase Gene for Mutations in Chinese Subjects with or without Hypertriglyceridemia

Objective： To investigate the association between the mutations in lipoprotein lipase gene and hypertriglyceridemia （HTG）. Methods： The lipoprotein lipase （LPL） gene was screened for mutations in 386 Chinese subjects with （108 cases in the HTG group） or without HTG （278 cases in the control group）, by single-strand conformation polymorphism （SSCP） analysis and DNA sequencing. Results： One novel silent mutation L103L, one missense mutation P207L, three splicing mutations Int3/3＇ -ass/C（-6）→T, and the common S447X polymorphism has been identified in the whole coding region and exon-intron junctions of the LPL gene were examined. Heterozygous P207L found in the HTG group was the first case reported in Asia and subsequently another P207L heterozygote was found in the proband＇s family, all of which suggested that P207L was one of the causes of familial combined hyperlipidemia, but was not so prevalent as that in French Canadian. Int3/3＇-ass/C（-6）→T was found in both groups in the present study although it was regarded as a pathogenic variant to HTG earlier on. Moreover about the beneficial polymorphism S447X, there was also some supportive evidence that the levels of triglycerides （TG） in S447X carriers were significantly lower than noncarders in the subjects without HTG. Conclusions： The association between the LPL variants and HTG is quite complicated and versatile, genotyping of LPL in a larger-scale screening should be necessary and justifiable.

Determination of Isoflavone from Soybean Lines Cultivated in Jilin Province and Correlation Analysis between Isoflavone Content and Soybean Quality

[Objective]The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat content. [Method]The isoflavones were firstly extracted by 80% methanol and then hydrolyzed at 100 ℃. The chromatographic separation adopted a reversed-phase C18 analytical column with binary high-pressure gradient elution,while its analysis time was 25 min and column temperature was 40 ℃. The diode array detector was used for monitoring with wavelength of 260 nm. The correlation between isofalvone content and protein or fat content was analyzed by data processing system Origin 6.0. [Result]The high performance liquid chromatograph for determination of isoflavones from soybean was verified to be accurate and reliable by methodology. The isoflavones of 85 soybean lines cultivated in Jilin Province were determined,and the results primarily showed the characters and ranges of isoflavones from soybean lines cultivated in Jilin Province,while the isoflavone content of soybeans ranged from 2.29 to 4.89 mg/g,and the average content was 3.36 mg/g. The isoflavone content of 5 soybean lines exceeded 4 mg/g,while there was a remarkably negative correlation between isoflavone content and protein content,and there was no significant positive correlation between isoflavone content and fat content. [Conclusion]The isoflavone content of soybean lines cultivated in Jilin Province is higher,so it is feasible for breeding the soybean lines with high isoflavone content and fat contetnt.

Correlation analysis between major nutritional components and resistant starch content in wheat

With 94 spring wheat cultivars as experimental materials, the correlations between the content of resistant starch （RS） in uncooked flour and cooked flour, and the apparent amylose content （AAC）, protein, lipid were investigated. The results showed that RS contents in both the uncooked flour and cooked flour assumed significantly positive correlation with AAC, and significantly negative cor- relation with protein content; and they were proved to be not significantly correlated with lipid content. RS content in uncooked flour was significantly correlated with that in cooked flour. These results provided references for the genetic improvement of wheat cultivars.

Sequence Analysis of Polymorphic Fragments in the Third Intron of Porcine H-FABP Gene

[ Objective] The aim of this study was to provide a theoretical basis for exploring the major genes affecting intramuscular fat （IMF） deposition. [Method] Taking 383 pigs from five breeds including Mashen Pig, Large White Pig, Landrace, Duroc and Shanxi White Pig as the experimental animals, polymorphisms of partial fragments in the third intron of porcine H-FABP gene were detected by PCR-SSCP method, and then the polymorphic fragments were sequenced. [ Result] Two alleles, designated as A and B, were found at the locus 346 in the third intron of porcine H-FABP gene, and the mutation was caused by a A→G substitution. [ Conclusion] A polymorphic locus was discovered in the third intron of porcine H-FABP gene in this experiment, laying a foundation for the further study on the relationship between H-FABP gene and IMF content.

Quality Analysis and Evaluation of Maize Cultivars Approved by Shanxi Province

[Objective] This study aimed to provide a theoretical basis for the genetic improvement of maize quality in Shanxi Province. [Method] The variations in unit weight, crude starch content, crude protein content, crude fat content and lysine content of maize cultivars approved by Shanxi Province during 2003-2012 were analyzed. [Result] In Shanxi Province, the average unit weight and crude starch content of maize cultivars approved during 2003-2012 were trended to be increased;the crude fat contents of maize cultivars approved during 2003-2012 showed no significant changes; the lysine contents of maize cultivars approved during 2003-2008 were essentially unchanged; the crude protein contents of maize cultivars approved during 2003-2012 declined slightly. [Conclusion] The crude starch content and unit weight of maize increased with the increased yield; the crude protein content, crude fat content and lysine content showed certain stability, and they were mainly controlled by genes. Therefore, the breeding of particular maize should be strengthened.

Periodic Analysis on Nutrients in Chinese Residents and Their Sources

The nutritional status of Chinese residents has been greatly improved during the past 50 years.Compared with Japanese people to whom our food consumption habit is similar,the calorie nutrient intake and fat nutrient intake per capita of Chinese residents has exceeded the intake of the Japanese residents,but the protein nutrient intake is still lower.Among the composition of the calorie nutrient,the proportion of fat is higher,but the proportion of protein is lower,and both the animal protein and the vegetable fat are small.Therefore,we should guide the poor to the rational consumption such as soybean which contains quality protein but is a kind of inexpensive food.With the increase of population eating out,residents＇ nutrients awareness as well as the public service institutions knowledge of nutrition should be enhanced.

首个基因治疗药物Glybera获欧委会批准上市

2012年11月2日,uniQure生物技术公司宣布,其研发的Glybera（alipogene tiparvovec）获欧盟委员会批准,用于治疗胰腺炎反复发作导致的脂蛋白脂肪酶缺乏症（LPLD）。

Assimilating efficiency of soil nutrition of Phalaris arundinacea L.

Phalaris arundinacea L. is fine perennial herbage of Gramineae. Eight treatments including N, P, K, NP, NK, NPK, PK and controls were done for studying its absorbing efficiency of soil nutrition. At tillering stage, the coefficient of absorbing and utilizing of N, P, and K from soil and fertilizer were analyzed according to M.K. Kaeomobk抯 formula and the contents of crude protein and fat were also measured. The yields of fresh crop, hay and seed were measured at ripening stage. For N absorbing coefficient of Ph. arundinacea from soil (Kn%), the composition PK treatment is best (12.36%) and K treatment is worse, while for N absorbing coefficient from fertilizer (Ky%), all the treatments are higher than control but the composition NPK treatment presented the highest (14.78%). For the absorbing coefficient of P from soil (Kn%), composition NK treatment is highest (19.30%), but K treatment was lower than control, while for that of P from fertilizer (Ky%), all the treatment were higher than control but the composition NP treatment showed the highest (19.52%). As to K absorbing coefficient from soil (Kn%), NP treatment is the best (19.2%) and single P treatment is worst, while for that (Ky%) of K from fertilizer the composition NPK treatment was best (28.44%). As concerns treatments for the outputs of fresh and dry crop, the composition NP treatment was best and all the treatment were higher than control. The composition NK treatment produced highest yield of seed and highest content of crude protein as compared to other treatments. For content of crude fat, the compositions NK and N treatments are best. The experiment indicates that application of fertilizer at proper proportions could increase the yield of Ph. Arundinacea and raise the utilizing efficiency of nourishment element from soil and fertilizer.

Effect of Rhizobium Inoculation on Phosphorus Uptake,Yield and Quality of Soybean Plants

[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46 as experimental soybeans, four treatments were designed, including non-inoculated CK group and three treatment groups inoculated respectively with rhizobium strains R2, R, and Rs, to analysis the effects of rhizobium inoculation on growth status, phosphorus uptake, yield and quality of soybean plants. [Result] Inoculation with different rhizobium strains could significantly increase the biomass and phosphorus content of the roots and shoots of Dongnong 42, to be specific, rhizobium strain R2 showed the best ef- fect, followed by Rs, and those of roots were improved greater than the shoots; in- oculation with rhizobium could significantly increase the yield of Dongnong 42, and R5 showed the best effect, but the yield of Dongnong 46 was decreased; after in- oculated with rhizobium, the protein content and fat content of soybean were signifi- cantly increased, and R2 showed the best effect, but the effects on different varieties varied, to be specific, the protein content of Dongnong 42 was significantly in- creased, while the fat content of Dongnong 46 was significantly increased; different rhizobium strains inoculated to different soybean varieties showed different effects. Inoculation with suitable rhizobium can improve plant growth characteristics, promote phosphorus uptake of plants, increase soybean yield, and effectively improve soy- bean quality. [Conclusion] This study provides theoretical basis for solving the prob- lem of soil phosphorus deficiency, increasing soybean yield and improving soybean quality in the future.

甘油三酯偏高应复查一次

我母亲今年52岁，身体一直挺好。前几天去体检，血脂四项中甘油三酯是5.9毫摩尔／升，其他都正常，请问她是否该吃点药调节一下？首先要强调，化验前一天不能吃油腻食物或饮酒，必须空腹12小时以上再去抽血化验。这是因为食物中的脂肪被人体吸收后经脂蛋白脂肪酶消化，正常人约需8小时才能使血液中的甘油三酯恢复到进餐前水平。

低脂高纤饭盒免痴肥

全国有近六成学生午餐全凭饭盒搞掂,由于学生们不注意均衡营养,又注重兴趣和口感,以致摄入过高脂肪低纤维饮食,造成一部分学生痴肥,实在令人担心。要挽救此危机。