作者采用骨髓染色体制片方法已对云南白腹鼠(Ruttus coxingi)的常规核型作了简报。其核型包括17对端着丝粒染色体、4对亚中着丝拉染色体、4对中着丝粒染色体、1对性染色体以及4个或6个 B 染色体。不同于 Makino 等人早年的报道.在这一...作者采用骨髓染色体制片方法已对云南白腹鼠(Ruttus coxingi)的常规核型作了简报。其核型包括17对端着丝粒染色体、4对亚中着丝拉染色体、4对中着丝粒染色体、1对性染色体以及4个或6个 B 染色体。不同于 Makino 等人早年的报道.在这一基础上.我们又对该种动物进行了染色体 C 显带和银染色研究,C 带所显示出的异染色质区域主要分布于端着丝粒染色体的着丝粒末端,而银染结果表明,核仁组织者区位于16号和20号染色体上,获得了其染色体内在结构上的一些特征。另外,文章还讨论了 B 染色体在家鼠属(Genus Rattus)动物核型中的分布情况.为研究家鼠属动物染色体增添了新的资料。展开更多
Rotavirus diarrhea is a major worldwide cause of infantile gastroenteritis; however, the mechanism responsible for intestinal fluid loss remains unclear. Water transfer across the intestinal epithelial membrane seems ...Rotavirus diarrhea is a major worldwide cause of infantile gastroenteritis; however, the mechanism responsible for intestinal fluid loss remains unclear. Water transfer across the intestinal epithelial membrane seems to occur because of aquaporins(AQPs). Accumulating evidence indicates that alterations in AQPs may play an important role in pathogenesis. Here, we focus on changes in AQPs in a mouse model of rotavirus diarrhea. In the present study, 32 of 35 mice developed diarrhea and mild dehydration within 24 hours after infection with rotavirus strain SA11. Intestinal epithelial cells demonstrated cytoplasmic vacuolation, malaligned villi, and atrophy. AQP1 expression was significantly attenuated in the ileum and colon in comparison with controls; likewise, AQP4 and-8 protein expression were significantly decreased in the colon of rotavirus diarrhea-infected mice. In contrast, AQP3 protein expression was significantly increased in the colon of rotavirus-infected mice in comparison with controls. These results indicate that rotavirus diarrhea is associated with the downregulation of AQP1,-4, and-8 expression. Therefore, AQPs play an important role in rotavirus diarrhea.展开更多
cAMP mediated signaling may play a suppressive role in immune response. We previously found that the cAMP-elevators (CTx and 8-Br-cAMP) inhibited IL-12, IL-la, IL-6 gene expression, but increased the transcriptional l...cAMP mediated signaling may play a suppressive role in immune response. We previously found that the cAMP-elevators (CTx and 8-Br-cAMP) inhibited IL-12, IL-la, IL-6 gene expression, but increased the transcriptional levels of IL-10 and IL-IRa in LPS-treated murine peritoneal macrophages. The present study examined a possible molecular mechanism involved in cAMP elevators-induced inhibition of IL-12 p40 expression in response to LPS. Our data demonstrated that cAMP elevators downregulated IL-12 p40 mRNA expression and IL-12 p70 production in murine peritoneal macrophages. Subsequent studies revealed that cAMP-elevators blocked phosphorylation of p38 MAPK, but did not affect the activity of NF-kB binding to IL-12 promoter (-136/-112). This is the first report that cAMP elevators inhibit LPS-induced IL-12 production by a mechanism that is associated, at least in part, with p38-dependent inhibition by cAMP signaling pathways.展开更多
Objective Benzophenone semicarbazone(BSC) was synthesized and characterized to identify compounds with anticancer activities. Methods Anticancer activities were studied against Ehrlich Ascites Carcinoma(EAC) cells in ...Objective Benzophenone semicarbazone(BSC) was synthesized and characterized to identify compounds with anticancer activities. Methods Anticancer activities were studied against Ehrlich Ascites Carcinoma(EAC) cells in Swiss albino mice by monitoring parameters such as tumor weight measurement,survival time of tumor bearing mice,tumor cell growth inhibition,and so on.Some hematological parameters,such as red blood cells,white blood cells,and hemoglobin content,were also measured. Results The results showed that BSC has a positive effect against EAC cells.An assessment was conducted by comparing these results with those obtained using the standard drug bleomycin. Conclusions The BSC compound can be considered as a potent anticancer agent.展开更多
To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at ...To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at (100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw) against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters (e.g., hemoglobin content, red and white blood cells count and differential cell count) after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose (P 〈 0.05). It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.展开更多
The leaves of Aquilaria sinensis(Lour.) Gilg have been used in folk medicine for the treatment of diabetes in Guangdong Province,China.In this study,effects of ethanol extract of Aquilaria sinensis leaves on hypergl...The leaves of Aquilaria sinensis(Lour.) Gilg have been used in folk medicine for the treatment of diabetes in Guangdong Province,China.In this study,effects of ethanol extract of Aquilaria sinensis leaves on hyperglycemia were investigated in diabetic db/db mice.After 4 weeks of administration,the 95%ethanol(EtOH) extract of Aquilaria sinensis leaves (AE),especially at high dose level(600 mg/kg),activated AMP-activated protein kinase(AMPK),resulting in reduced fasting blood glucose and glycosylated hemoglobin levels in db/db mice.In addition,the oral glucose tolerance test(OGTT test) showed that AE could remarkably improve insulin resistance.Compared to Thiazolinediones(TZDs),no weight gain was observed after AE administration,which is a severe side effect of TZDs.The data suggested that AE could act as an activator of AMPK,and might be used as an alternative to TZDs in the management of obesity-related diabetes.展开更多
文摘作者采用骨髓染色体制片方法已对云南白腹鼠(Ruttus coxingi)的常规核型作了简报。其核型包括17对端着丝粒染色体、4对亚中着丝拉染色体、4对中着丝粒染色体、1对性染色体以及4个或6个 B 染色体。不同于 Makino 等人早年的报道.在这一基础上.我们又对该种动物进行了染色体 C 显带和银染色研究,C 带所显示出的异染色质区域主要分布于端着丝粒染色体的着丝粒末端,而银染结果表明,核仁组织者区位于16号和20号染色体上,获得了其染色体内在结构上的一些特征。另外,文章还讨论了 B 染色体在家鼠属(Genus Rattus)动物核型中的分布情况.为研究家鼠属动物染色体增添了新的资料。
基金supported by funding from Guangdong Natural Science Foundation (grants:S2012010009211,S2012010009538)Key Specialty Projects of Guangzhou Board of Health (grant:20121A021014)
文摘Rotavirus diarrhea is a major worldwide cause of infantile gastroenteritis; however, the mechanism responsible for intestinal fluid loss remains unclear. Water transfer across the intestinal epithelial membrane seems to occur because of aquaporins(AQPs). Accumulating evidence indicates that alterations in AQPs may play an important role in pathogenesis. Here, we focus on changes in AQPs in a mouse model of rotavirus diarrhea. In the present study, 32 of 35 mice developed diarrhea and mild dehydration within 24 hours after infection with rotavirus strain SA11. Intestinal epithelial cells demonstrated cytoplasmic vacuolation, malaligned villi, and atrophy. AQP1 expression was significantly attenuated in the ileum and colon in comparison with controls; likewise, AQP4 and-8 protein expression were significantly decreased in the colon of rotavirus diarrhea-infected mice. In contrast, AQP3 protein expression was significantly increased in the colon of rotavirus-infected mice in comparison with controls. These results indicate that rotavirus diarrhea is associated with the downregulation of AQP1,-4, and-8 expression. Therefore, AQPs play an important role in rotavirus diarrhea.
基金supported by Shanghai Institutes for Biological Sciences,Chinese Academy of Sciencesthe grant from Ministry of Scienceand Technology(G1999054201)National Natural Science Foundation of China(39870717).
文摘cAMP mediated signaling may play a suppressive role in immune response. We previously found that the cAMP-elevators (CTx and 8-Br-cAMP) inhibited IL-12, IL-la, IL-6 gene expression, but increased the transcriptional levels of IL-10 and IL-IRa in LPS-treated murine peritoneal macrophages. The present study examined a possible molecular mechanism involved in cAMP elevators-induced inhibition of IL-12 p40 expression in response to LPS. Our data demonstrated that cAMP elevators downregulated IL-12 p40 mRNA expression and IL-12 p70 production in murine peritoneal macrophages. Subsequent studies revealed that cAMP-elevators blocked phosphorylation of p38 MAPK, but did not affect the activity of NF-kB binding to IL-12 promoter (-136/-112). This is the first report that cAMP elevators inhibit LPS-induced IL-12 production by a mechanism that is associated, at least in part, with p38-dependent inhibition by cAMP signaling pathways.
基金supported by a grant from the National Science and Information and Communication Technology of Bangladesh
文摘Objective Benzophenone semicarbazone(BSC) was synthesized and characterized to identify compounds with anticancer activities. Methods Anticancer activities were studied against Ehrlich Ascites Carcinoma(EAC) cells in Swiss albino mice by monitoring parameters such as tumor weight measurement,survival time of tumor bearing mice,tumor cell growth inhibition,and so on.Some hematological parameters,such as red blood cells,white blood cells,and hemoglobin content,were also measured. Results The results showed that BSC has a positive effect against EAC cells.An assessment was conducted by comparing these results with those obtained using the standard drug bleomycin. Conclusions The BSC compound can be considered as a potent anticancer agent.
文摘To evaluate the antitumor activity of Lactuca serriola against EAC (Ehrlich ascites carcinoma) in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at (100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw) against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters (e.g., hemoglobin content, red and white blood cells count and differential cell count) after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose (P 〈 0.05). It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.
基金New-Century Talent Program,Ministry of Educating of China(Grant No.985-2-102-113)National Key Technology R&D Program"New Drug Innovation"of China(Grant No.2009ZX09311-004,2012ZX09301-002-002-002)
文摘The leaves of Aquilaria sinensis(Lour.) Gilg have been used in folk medicine for the treatment of diabetes in Guangdong Province,China.In this study,effects of ethanol extract of Aquilaria sinensis leaves on hyperglycemia were investigated in diabetic db/db mice.After 4 weeks of administration,the 95%ethanol(EtOH) extract of Aquilaria sinensis leaves (AE),especially at high dose level(600 mg/kg),activated AMP-activated protein kinase(AMPK),resulting in reduced fasting blood glucose and glycosylated hemoglobin levels in db/db mice.In addition,the oral glucose tolerance test(OGTT test) showed that AE could remarkably improve insulin resistance.Compared to Thiazolinediones(TZDs),no weight gain was observed after AE administration,which is a severe side effect of TZDs.The data suggested that AE could act as an activator of AMPK,and might be used as an alternative to TZDs in the management of obesity-related diabetes.