全自动血液成分分离机白膜法制备浓缩血小板相关影响因素的分析

目的:探讨影响全自动血液成分分离机制备浓缩血小板(简称机分血小板)质量的相关因素,确定理想的制备条件。方法:分别比较离心机的参数、全自动血液成分分离机的参数对血小板质量的影响。结果:重离心RCF2100g,14min,轻离心RCF280g,10min,慢加速5档,缓减速3档,分离效果明显好于重离心RCF2800g,19min,轻离心RCF300g,6min,慢加速7档,缓减速5档。白膜量45g,血小板计数合格率显著高于43g。结论:重离心RCF2100g,14min,轻离心RCF280g,10min,慢加速5档,缓减速3档,白膜毛重95g,净重45g为一组白膜法机分血小板的较理想的制备参数。

全自动血液成分分离仪白膜法制备血小板的参数探讨

目的采用全自动血液成分分离仪,用白膜法制备浓缩血小板,寻找理想的制备条件。方法分别比较两种离心力及白膜量对血小板和悬浮红细胞质量的影响。结果白膜量43g,血小板计数及回收率均显著高于42g(P<0.01),悬浮红细胞中白细胞残存率显著低于42g(P<0.01)。两种离心力对血小板及悬浮红细胞质量的影响无显著性差异(P>0.05)。结论2236×g、白膜量43g为白膜法机器分离血小板的最佳制备参数。

Proteomic study provides new clues for complications of hemodialysis caused by dialysis membrane

The complications of hemodialysis accompanied the hemodialysis and threaten the patients’life.Besides the loss of nutrient substance,such as amino acid and vitamin,we found new clues that the adsorbed proteins on common-used polysulfone-based dialysis membrane might be the reason according to the qualitative proteomic study by ionic liquid assisted sample preparation method.Our results indicated that the adsorbed proteins on the membrane were related with complement activation,blood coagulation,and leukocyte-related biological process.The quantitative proteome further demonstrated some significant changes of signal proteins in the post-dialysis plasma after the hemodialysis,such as beta-2-microglobulin and platelet factor-4,which would further verify these new clues.