In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction ...In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction time on polyphenols extraction rate were investigated. On the basis of single-factor test, a four-factor and three-level orthogonal test was designed by response surface method to establish a mathematical model between the response value and various factors. The results showed that the intensity of effect of different influencing factor on polyphenols ex- traction rate ranked as microwave power＇s〉solid to liquid ratio＇s〉extraction time＇s〉 ethanol concentration＇s. The optimum microwave-assisted extraction conditions for polyphenols from L. chinense were as follows： extraction power 254 W, ethanol concentration 60%, extraction time 12.5 rain and solid to liquid ratio 1：17. Under the optimum extraction conditions, the extraction rate of polyphenols from L. chinense was 19.17%.展开更多
In the present study, four different proteases (pepsin, papain, bromelain and ficin) were screened with a murine monoclonal antibody OC859 , in order to verify whether different digestion procedures could improve yiel...In the present study, four different proteases (pepsin, papain, bromelain and ficin) were screened with a murine monoclonal antibody OC859 , in order to verify whether different digestion procedures could improve yield and stability of the F(ab’)2 or Fab fragments. The yields of F(ab’)2 or Fab fragments from digestion with pepsin, papain , bromelain and ficin were respectively 20. 3+/-2. 0%, 50. 5+/-5. 0%,74. 4+/-2. 7% and 82. 8 +/-10. 2% of the theoretical maximum. Immunoreactivity in a noncompetitive solid-phase radioimmunoassay (SPRIA) of the fragments generated by the four proteases were respectively 10+/-5%, 36+/-5%, 60+/-6% and 75+/-6%of the intact OC859 IgG. These results suggested that the fragmentation of OC859 with ficin gave a higher yield of superior immunoreactive fragments.展开更多
基金Supported by Open Funds for Innovation Platforms of Colleges and Universities in Hunan Province(15K066)National College Students'Innovative Entrepreneuria Training Program(201510553003)Research Study and Innovative Experiment Plan Project for College Students in Hunan Province(2015-499)~~
文摘In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction time on polyphenols extraction rate were investigated. On the basis of single-factor test, a four-factor and three-level orthogonal test was designed by response surface method to establish a mathematical model between the response value and various factors. The results showed that the intensity of effect of different influencing factor on polyphenols ex- traction rate ranked as microwave power＇s〉solid to liquid ratio＇s〉extraction time＇s〉 ethanol concentration＇s. The optimum microwave-assisted extraction conditions for polyphenols from L. chinense were as follows： extraction power 254 W, ethanol concentration 60%, extraction time 12.5 rain and solid to liquid ratio 1：17. Under the optimum extraction conditions, the extraction rate of polyphenols from L. chinense was 19.17%.
文摘In the present study, four different proteases (pepsin, papain, bromelain and ficin) were screened with a murine monoclonal antibody OC859 , in order to verify whether different digestion procedures could improve yield and stability of the F(ab’)2 or Fab fragments. The yields of F(ab’)2 or Fab fragments from digestion with pepsin, papain , bromelain and ficin were respectively 20. 3+/-2. 0%, 50. 5+/-5. 0%,74. 4+/-2. 7% and 82. 8 +/-10. 2% of the theoretical maximum. Immunoreactivity in a noncompetitive solid-phase radioimmunoassay (SPRIA) of the fragments generated by the four proteases were respectively 10+/-5%, 36+/-5%, 60+/-6% and 75+/-6%of the intact OC859 IgG. These results suggested that the fragmentation of OC859 with ficin gave a higher yield of superior immunoreactive fragments.
