白花蛇舌草诱导骨髓瘤8226细胞凋亡的研究

目的：通过对白花蛇舌草（hedyotis diffusa,HD）在体外抑制骨髓瘤细胞株RPMI8226的增殖作用的观察,探讨HD治疗骨髓瘤患者的作用机制。方法：以多发性骨髓瘤细胞株（RPMI8226）为研究对象,应用MTT法观察不同浓度HD对RPMI8226细胞增殖的影响作用,用AV/PI流式细胞检测术观察细胞凋亡,Hoechst染色观察形态学改变。结果：HD在1~4mg/mL浓度范围内对RPMI8226细胞有明显的增殖抑制作用,最高抑制率达到了92.68%,呈时间、剂量依赖关系。以1、2、3mg/mL的HD分别作用RPMI8226细胞24h后,AV/PI流式细胞仪分析结果显示早期凋亡率分别为：22.52%、62.31%、69.94%,高于空白对照组8.93%。HD诱导RPMI8226细胞凋亡时,细胞核可见凋亡小体,且凋亡小体数量随HD浓度增加而增加。结论：HD在一定浓度下可抑制骨髓瘤细胞增殖,其作用机理可能是诱导细胞凋亡。此实验研究结果为HD作为治疗多发性骨髓瘤的辅助药物提供实验依据。

Threat of Domestic Power Generators to Terrestrial Ecosystem as Expressed in Some Plant Species

Polluted populations of Euphorbia heterophylla, Chromolaena odorata, Commelina diffusa and Kyllinga pumila were collected fresh from 0.1 m to 0.25 m radii of the exhausted-pipe of power generators used for domestic purposes, whereas, their non-polluted counterparts were collected from distances of 100-110 m away. The length and width of 10 leaves, each of both polluted and non-polluted populations, were measured. Their average leaf areas （LA） were 2.60 ± 0.1 cm2, 14.16 ±0.4 cm2, 5.19 ± 0.8 cm2 and 1.80 ±0.8 cm2 for the polluted populations of each of the species, respectively. Whereas, the LA of their non-polluted counterparts were 4.18±0.7 cm2, 34.39 ±0.2 cm2, 7.51± 0.1 cm2 and 10.76 ± 0.3 cm2, respectively. Damages such as plugged stomatal pores, epidermal cell aberrations and erosion, ruptured stomatal ledges, occasional leaf perforations, irregularly fused cell boundaries and glued leaf surfaces were noticeable in the leaves of the polluted populations. Such were not observed in the non-polluted populations. It was suggested that foliar morphology of these group of plants could serve as phytometer to gauge the threats of power generators used in homes to terrestrial ecosystem.