[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR produ...[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.展开更多
AIM To evaluate the role of cAMP/PKA and DAG/PKC pathways of the MGc80 3 cells treated with traditional Chinese medicine of compound Bailong preparation (Bailong).
Our expedition have identified location in the central part of Kosovo where were collected a samples （accessions） at coordinates; Sub-Locality SL-1 （N42°34′36″, E21°07′45″）, altitude 574 m, SL-2 （N4...Our expedition have identified location in the central part of Kosovo where were collected a samples （accessions） at coordinates; Sub-Locality SL-1 （N42°34′36″, E21°07′45″）, altitude 574 m, SL-2 （N42°34′29″, E 21°07′45″altitude 591 m and SL-3 （N42°34′30″, E21°07′46″） on altitude 565 m. The aim of present investigation was carried out to study genetic variability of quantitative and qualitative parameters. The populations from SL-2 were characterized of higher significance on seed oil （23.74%） and protein content （16.74%）. The estimation average value of seed protein and oil content was 15.52% and 23.48% respectively. The variability of plant height ranged from 182.33 cm to as much as 200.89 cm, while distinction＇s between them were ＋ 18.56 cm which was a significant higher at P〈0.05 probability level. The SL-3 showed the higher value of HWK （34.6 g）, head per plant （39.33） and head diameter （9.5 cm） but, was characterized significantly lowest on seed protein content （13.99%）. The all traits included in our investigations showed a higher genetic variability with majority of extreme values. The sunflower populations differed significantly in several parameters measured, they have a different genetic and morphological backgrounds. Results from wild sunflower form indicated notable differences in dispersion of important genes for different traits and showed the potential for using in future for breeding program.展开更多
基金Supported by Research Fund of the Doctoral Program of Higher Education (200805720004)Scientific Research Foundation for Returned Scholars, Ministry of Education of China ([2009]1001)~~
文摘[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.
文摘AIM To evaluate the role of cAMP/PKA and DAG/PKC pathways of the MGc80 3 cells treated with traditional Chinese medicine of compound Bailong preparation (Bailong).
文摘Our expedition have identified location in the central part of Kosovo where were collected a samples （accessions） at coordinates; Sub-Locality SL-1 （N42°34′36″, E21°07′45″）, altitude 574 m, SL-2 （N42°34′29″, E 21°07′45″altitude 591 m and SL-3 （N42°34′30″, E21°07′46″） on altitude 565 m. The aim of present investigation was carried out to study genetic variability of quantitative and qualitative parameters. The populations from SL-2 were characterized of higher significance on seed oil （23.74%） and protein content （16.74%）. The estimation average value of seed protein and oil content was 15.52% and 23.48% respectively. The variability of plant height ranged from 182.33 cm to as much as 200.89 cm, while distinction＇s between them were ＋ 18.56 cm which was a significant higher at P〈0.05 probability level. The SL-3 showed the higher value of HWK （34.6 g）, head per plant （39.33） and head diameter （9.5 cm） but, was characterized significantly lowest on seed protein content （13.99%）. The all traits included in our investigations showed a higher genetic variability with majority of extreme values. The sunflower populations differed significantly in several parameters measured, they have a different genetic and morphological backgrounds. Results from wild sunflower form indicated notable differences in dispersion of important genes for different traits and showed the potential for using in future for breeding program.
