目的:制备多柔比星磁性白蛋白纳米微粒(adriamycin magnetic albumin nanoparticles,ADM-MANs)。方法:利用人血清白蛋白、多柔比星、中位直径25 nm的Fe3O4磁粉,采用热固化交联和油剂分散法制备。光学显微镜和扫描电子显微镜观察其磁响...目的:制备多柔比星磁性白蛋白纳米微粒(adriamycin magnetic albumin nanoparticles,ADM-MANs)。方法:利用人血清白蛋白、多柔比星、中位直径25 nm的Fe3O4磁粉,采用热固化交联和油剂分散法制备。光学显微镜和扫描电子显微镜观察其磁响应性和结构。应用荧光分光光度法检测磁性微粒中多柔比星含量。体外实验用MTT法检测成品微粒的细胞毒作用。结果:制备出的ADM-MANs具有磁性药物微球的形态学及物理学特征。4 000高斯磁场强度下磁响应性距离为80-100 mm。微粒直径50-400 nm,中位直径200 nm。1 mg ADM-MANs含多柔比星30．5μg,药物包封率为61．2％。其对SMMC7721肿瘤细胞的抑制率与在相应浓度下的游离多柔比星相近,半数抑制浓度分别为0．327μg·mL-1(以多柔比星含量计)和0．312μg·mL-1,统计学检验无显著性差异(P>0．05)。结论:制备出的ADM-MANs粒径小、分布均匀、磁响应性良好、药物包封率高,多柔比星细胞毒活性无明显改变。展开更多
The spherical silver mirco/nano-particles with narrow size distributions were obtained by chemical reduction of silver ammonia solution with ascorbic acid as reducing agent and bovine serum albumin (BSA) as bio-templa...The spherical silver mirco/nano-particles with narrow size distributions were obtained by chemical reduction of silver ammonia solution with ascorbic acid as reducing agent and bovine serum albumin (BSA) as bio-template. The effects of the concentration of Ag+ ions, BSA and ammonia, reactive temperature on the silver morphology and particle sizes were investigated. SEM, TEM and XRD were employed to characterize the morphology and structures of the prepared silver mirco/nano-particles. The results show that the spherical silver particle with smooth surface and narrow size distributions can be obtained by controlling the concentration of Ag+ ions, BSA, reaction temperature, etc. By controlling the above parameters, the silver spherical mirco/nano-particles with particle sizes ranging from 0.2 to 2.3 m can be well prepared, which is expected to be used in manufacturing high performance electronic pastes.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 51271135) "New Century Talents Project" of Ministry of Education and Fundamental Research funding of Xi'an Jiaotong Universitythe project of Innovative Team of Shaanxi Province (Grant No. 2013KCT-05)
文摘The spherical silver mirco/nano-particles with narrow size distributions were obtained by chemical reduction of silver ammonia solution with ascorbic acid as reducing agent and bovine serum albumin (BSA) as bio-template. The effects of the concentration of Ag+ ions, BSA and ammonia, reactive temperature on the silver morphology and particle sizes were investigated. SEM, TEM and XRD were employed to characterize the morphology and structures of the prepared silver mirco/nano-particles. The results show that the spherical silver particle with smooth surface and narrow size distributions can be obtained by controlling the concentration of Ag+ ions, BSA, reaction temperature, etc. By controlling the above parameters, the silver spherical mirco/nano-particles with particle sizes ranging from 0.2 to 2.3 m can be well prepared, which is expected to be used in manufacturing high performance electronic pastes.
