急性脑血管病患者血清白介素-8测定的临床意义

目的 了解急性脑血管病患者血清白介素－ ８（ Ｉ Ｌ － ８） 改变的意义。方法 采用 Ｅ Ｌ Ｉ Ｓ Ａ 方法测定了３０ 例急性脑血管病患者（１６ 例脑出血，１４ 例 脑梗死） 和３０ 例健康 对照者的血清 Ｉ Ｌ － ８ 含量。结果 急性 脑血管病患者血清 Ｉ Ｌ － ８ 水 平（９７５４ ±３０８１ ｎｇ／ Ｌ） 明显高于正常对照组（５１３５ ±１３２９ ｎｇ／ Ｌ） 。急性脑出血和急性脑梗死组患者血清 Ｉ Ｌ － ８ 水平（１０５２８ ±１８９１ ｎｇ／ Ｌ 和９４８９ ±３２１４ ｎｇ／ Ｌ） 均明显高于正常对照组，而它们之间则无明显差别。结论 急性脑血管病患者 急性期血清 Ｉ Ｌ － ８ 水平 明显升高，提示 Ｉ Ｌ － ８ 可能参与了急性脑血管病的发病过程。

Expression change of interleukin-8 gene in rabbit basilar artery after subarachnoid hemorrhage

Objective To study the expression change of interleukin-8 （IL-8） gene in the basilar artery of rabbit and the effect of IL-8 on the development of cerebral vasospasm induced by experimental subarachnoid hemorrhage （SAH）. Methods Thirty five healthy Japanese White Rabbits were randomly divided into saline-control group and experimental group. The experimental group was subdivided into four groups, representing day 1, 4, 7 and 14 after the first blood injection of SAH. The delayed cerebral vasospasm （DCVS） model was established by double injection of autologous blood into the cisterna magna. The expression change of cytokine IL-8 mRNA in the basilar artery was analyzed by RT- PCR. Results The expression of IL-8 gene increased on day 4-7 after the first blood injection of SAH compared with control （P 〈 0.001）, and decreased to normal on day 14. The expression of IL-8 gene in the SAH groups were positively correlated with the degree of basilar artery stenosis （r = 0.642, P 〈 0.01）. Conclusion The expression level of IL-8 gene in basilar arteries was intimately associated with the degree of cerebral vasospasm, suggesting that IL-8 may play an important role in the DCVS after SAH as an immunological inflammatory factor.

Electroacupuncture on serum interleukin level in rat models of cerebral ischemia-reperfusion injury

Objective： To observe the effect of electroacupuncture （EA） on serum interleukin （IL）-6, IL-8 and IL-10 in rat models of cerebral ischemia-reperfusion, and to discover the mechanism of EA in preventing and treating cerebral ischemia. 〈br〉 Methods：Male Sprague Dawley （SD） rats were randomized into a sham-operation （SO） group, a model control （MC） group, and an EA group, which were sub-grouped into a 6-hour group and a 24-hour group. In the SO group, rats only received vessel separation with filament placed inside without any treatment. In the MC and EA groups, the focal cerebral ischemia-reperfusion model was induced by using modified Longa method with intraluminal filament. The MC group didn’t receive any treatment;the EA group received EA at Baihui （GV 20） and Dazhui （GV 14） with sparse-dense wave for 30 min. The levels of serum IL-6, IL-8 and IL-10 were detected by using Elisa test. 〈br〉 Results： Six hours after ischemia-reperfusion injury, the levels of serum IL-6, IL-8 and IL-10 in the MC group were significantly higher than those in the SO group （P〈0.01, P〈0.05, P〈0.05）;the level of serum IL-8 in the EA group was significantly lower than that in the MC group （P〈0.05）, while there were no significant differences in comparing IL-6 and IL-10 between the EA group and the MC group. Twenty-four hours after ischemia-reperfusion injury, the levels of serum IL-6 and IL-8 in the EA group were significantly lower than those in the MC group （both P〈0.05）, while there were no significant differences in comparing the level of IL-10 among the three groups. 〈br〉 Conclusion：Early intervention by EA can regulate the levels of serum IL-6 and IL-8 in cerebral ischemic injury.