再生障碍性贫血患者T细胞亚群和血清IL-2、IL-18水平检测的临床意义

目的：分析观察再生障碍性贫血（aplastic anemia,AA）患者外周血中 T 细胞亚群表达情况和血清中白介素-2（interleukin-2,IL-2）、白介素18（interleukin-18,IL-18）表达量,并探讨其检测的临床意义.方法：应用流式细胞仪检测法和酶联法对30例AA患者进行了T细胞亚群和血清IL-2、IL-18水平检测,同期与30例健康体检者作比较.结果：AA 患者组 CD3、CD4、CD4/CD8较健康体检组均降低,且具有统计学意义（P〈0.01）,CD8水平升高,IL-2、IL-18水平升高,都具有统计学意义（P〈0.01）,CD3水平与 IL-2水平呈显著负相关（r=-0.6018,P〈0.01）.结论：检测 T细胞亚群和血清 IL-2、IL-18水平的变化对判断 AA 病情、监测疗效均有重要的临床价值.

Serum soluble interleukin-2 receptor levels in patients with chronic hepatitis B virus infection and its relation with antiHBc

AIM： To investigate the relationship between serum soluble interleukin-2 receptor （sIL-2R） level and anti-HBc in patients with chronic hepatitis B virus （HBV） infection. METHODS： Sera from 100 patients with chronic HBV infection and 30 healthy controls were included in this study. The patients were divided into group A [HBsAg （＋）, HBeAg （＋） and anti-HBc （＋）, n = 50] and group B [HBsAg （＋）, HBeAg （＋） and anti-HBc （-）, n = 50]. sIL-2R levels were determined using ELISA. HBV DNA and alanine aminotransferase （ALT） were also detected. RESULTS： Serum sIL-2R levels were significantly higher in patients with chronic HBV infection than in healthy controls. Moreover, serum sIL-2R levels were significantly higher in patients with HBsAg （＋）, HBeAg （＋） and antiHBc （＋） （976.56±213.51×10^3 U/L） than in patients with HBsAg （＋）, HBeAg （＋） and anti-HBc （-） （393.41±189.54 ×10^3 U/L, P〈 0.01）. A significant relationship was found between serum sIL-2R and ALT levels （P〈 0.01） in patients with chronic HBV infection, but there was no correlation between sIL-2R and HBV DNA levels. The anti-HBc status was significantly related to the age of patients （P〈 0.01）. CONCLUSION： The high sIL-2R level is related to positive anti-HBc in chronic hepatitis B patients. Positive anti-HBc may be related to T-lymphocyte activation and negative anti-HBc may imply immune tolerance in these patients.

SERUM HAPTOGLOBIN SUPPRESSES T-LYMPHOCYTE FUNCTIONS FOLLOWING BURNS

It is well known that serum immunosuppressive factors play an important role in the mechanism of postburn immunosuppression.This study was intended to investigate the effect of haptoglobin, purified from the serum of burned patients by affinity chromatography,on the proliferation and interleukin-2(IL-2) secretion of normal murine thymocytes induced by ConA and the proliferation of IL-2 dependent cell line(CTLL-2) stimulated by recombinant human IL-2,so as to elucidate the role of serum haptoglobin in postburn T-lymphocyte dysfunction.The results showed that purified haptoglobin,at the level equivalent to the concentration found in serum of burned patients,significantly inhibited the proliferation and IL-2 secretion of normal murine thymocytes as well as CTLL-2 proliferation;whereas it exhibited no immunosuppressive effects at the level equivalent to the concentration found in serum of nomal volunteers.According to the results reported here,it is suggested that extraordinary increase in serum haptoglobin level may be an important factor of impaired T-lymphocyte responses following burns.

Production of Interleukin 2 like Growth Factor from the Mitogen Activated Peripheral Blood Lymphocytes of Indian Major Carp, Labeo rohita

Optimum conditions for in vitro production of interleukin 2 （IL 2） like activity from the Peripheral blood lymphocytes （PBL） of an Indian major carp, Labeo rohita were studied. Culture supernatants were generated by culturing the PBL in RPMI-1640 media supplemented with Glutamine and 10% fetal bovine serum （FBS） and stimulating with two different mitogens： concanavalin A （Con A） and Phytohaemagglutinin （PHA） at different concentrations separately. Significantly （P 〈 0.01） higher proliferation response was obtained from the culture supematant stimulated with concanavalin A （Con A） at a concentration of 10 lag mLl. The effect of phorbol myristate acetate （PMA） was also studied by co-stimulating PBL with Con A and PHA separately and it was found to synergistically enhancing the stimulation index with Con A whereas the stimulation index remain unchanged with PHA. The Con A （10 μg mLl） stimulated PBL were also cultured at different cell density, incubation period and incubation temperature in order to optimize the in vitro L. rohita IL2 production. The IL2 like activity was studied by lymphocyte proliferation assay on 72 h Con A blasts using WST based assay technique. Significantly （P 〈 0.01） higher stimulation indices were obtained when the PBL were cultured at a cell density of 1 × 10^6 cells mL^-1 for 30-36 h at an incubation temperature of 30 ℃. The IL2 like activity was purified by DEAE-Sepharose anion exchange chromatography and recorded between 70-130 mM NaCI with peak activity at 110 Mm NaCI. The molecular weight of the factor responsible for IL2 like activity was found to be 15-17 KD.

Reduction of serum level of interleukin-2 and pruritus severity after acupuncture at Quchi(LI11)in hemodialysis patients:a placebo-controlled randomized clinical trial

Objective To observe the effect of acupuncture at Quchi(LI11)on the serum interleukin-2(IL-2)level and the severity of uremic pruritus(UP)in hemodialysis patients.Methods Sixty hemodialysis patients with UP were randomized into an acupuncture group and a control group,with 30 cases in each group.In the acupuncture group,patients were treated with acupuncture at Quchi(LI11)twice a week for six weeks.In the control group,each patient received a placebo treatment(sham acupuncture).Before and after the intervention,the pruritis severity was examined using a 5-D scale,and the serum IL-2 level was measured using an enzyme-linked immunosorbent assay kit.Results There were no statistically significant differences in the demographic or clinical factors between the control group and the acupuncture group at baseline.The reduction in pruritus severity in patients was more significant in the acupuncture group(P=0.027).Acupuncture therapy also significantly decreased the serum IL-2 level in the patients(P=0.011).Throughout the acupuncture procedures,the incidence of pain and mild bleeding was 3.6%and 2.5%,respectively.Conclusion Six-week acupuncture treatment at Quchi(LI11)can effectively decrease the severity of UP and the serum IL-2 level.Large-scale,long-duration,and multi-center studies are needed to fully understand the role of IL-2 in inflammation and neuroimmune stimulation during acupuncture.