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Immunologic changes to autologous transfusion after operational trauma in malignant tumor patients:Neopterin and Interleukin-2 被引量:5
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作者 严敏 陈钢 +2 位作者 房玲玲 柳子明 张秀来 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第1期49-52,共4页
Objective:To estimate the impact of autologous transfusion on the status of perioperative immune activation inmalignant tumor patients.The Serum Neopterin and Interleukin-2(IL-2)were measured.Methods:Sixty patients un... Objective:To estimate the impact of autologous transfusion on the status of perioperative immune activation inmalignant tumor patients.The Serum Neopterin and Interleukin-2(IL-2)were measured.Methods:Sixty patients undergoingelective radical resection for malignant stomach tumor were enrolled in the prospective study and assigned to the following groups:(Ⅰ)Group A received autologous transfusion;(2)Group H received allogeneic transfusion.The perioperative course(Beforeinduction of anesthesia,after operation and 5 d after operation)of Neopterin and IL-2 was compared.Results:In group A,SerumNeopterin was significantly lower than baseline alter operation and IL-2 had no significant changes.In group H,both SerumNeopterin and IL-2 were significantly lower than baseline alter operation and 5 d after operation.Compared with group A,SerumNeopterin was significantly lower than baseline alter operation and 5 d after operation and IL-2 was significantly lower thanbaseline 5 d alter operation.Conchision:Autologous transfusion decreased the perioperative immune suppression in malignantstomach tumor patients. 展开更多
关键词 Autologous transfusion Allogeneic transfusion Serum Neopterin INF-gamma Immunoloic change
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High efficient mammalian expression and secretion of a functional humanized single-chain Fv/human interleukin-2 molecules 被引量:1
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作者 Yue-Chun Shen Xue-HaoWang +4 位作者 Xiao-Ming Wang Zao-Lai Chen Xi-Ping Shen Chao-Chen Zhao Jun Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第24期3859-3865,共7页
AIM: To construct and produce a recombinant bispecific humanized single-chain Fv (sFv) /Interleukin-2 (IL-2) fusion protein by using mammalian cells. METHODS: The sFv/IL-2 protein was genetically engineered, and... AIM: To construct and produce a recombinant bispecific humanized single-chain Fv (sFv) /Interleukin-2 (IL-2) fusion protein by using mammalian cells. METHODS: The sFv/IL-2 protein was genetically engineered, and transfected to mammalian cells to determine whether the mammalian protein folding machinery can produce and secrete active sFv/IL-2 with high efficiency. RESULTS: The fusion protein was constructed and high efficiently expressed with yields up to 102 ±4.2 mg/L in culture supernatant of the stably transfected 293 cell line. This recombinant fusion protein consisted of humanized variable heavy (VH) and light (VL) domains of monoclonal antibody (mAb) 520C9 directed against the human HER-2/neu (c-erbB2) proto-oncogene product p185, and human IL-2 connected by polypeptide linker. The fusion protein was shown to retain the immunostimulatory activities of IL-2 as measured by IL- 2-dependent cell proliferation and cytotoxicity assays. In addition to its IL-2 activities, this fusion protein also possessed antigen-binding specificity against p185, as determined by indirect ELISA using p185 positive SKOV 3ip1 cells. CONCLUSION: The large-scale preparation of the recombinant humanized sFv antibody/IL-2 fusion protein is performed with 293 cells. The recombinant humanized sFv antibody/IL-2 fusion protein may provide an effective means.of targeting therapeutic doses of IL-2 to p185 positive tumors without increasing systemic toxicity or immunogenicity. 展开更多
关键词 INTERLEUKIN-2 HUMANIZATION Antibody Fusion protein HER-2/NEU
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老化对人体白细胞功能的影响 被引量:1
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作者 王鑫 吴梧桐 《中国药科大学学报》 CAS CSCD 北大核心 1990年第4期215-218,共4页
本文比较了不同年龄不同性别的人体外周血白细胞在PHA诱导下DNA和蛋白质合成的能力及IL-2的产生水平。结果表明,在30岁至65岁年龄组之间,上述三项指标均平行下降,DNA合成下降50%,蛋白质合成下降43%,IL-2水平下降47%;同时发现该三项指标... 本文比较了不同年龄不同性别的人体外周血白细胞在PHA诱导下DNA和蛋白质合成的能力及IL-2的产生水平。结果表明,在30岁至65岁年龄组之间,上述三项指标均平行下降,DNA合成下降50%,蛋白质合成下降43%,IL-2水平下降47%;同时发现该三项指标在两性间似无明显差异。说明在人体衰老过程中人外周血白细胞的IL-2产生明显下降,这可能与老龄人群免疫功能系统功能降低有关。 展开更多
关键词 白间素-2 人外周血白细胞 衰老
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Expression of prolactin receptor and response to prolactin stimulation of human NK cell lines 被引量:4
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作者 RuiSUN AiLingLI +1 位作者 HaiMingWEI ZhiGangTIAN 《Cell Research》 SCIE CAS CSCD 2004年第1期67-73,共7页
We have previously shown a critical role of prolactin (PRL) during maturation and anti-tumor effects of murine natural killer (NK) cells in vitro and in vivo. We extended that study by exploring the ability of human N... We have previously shown a critical role of prolactin (PRL) during maturation and anti-tumor effects of murine natural killer (NK) cells in vitro and in vivo. We extended that study by exploring the ability of human NK cell lines (NK-92 and YT cell) to express PRL receptor (PRL-R) and to respond to PRL stimulation in vitro. Both human NK cell lines constitutively expressed PRL-R on membrane and mRNA transcripts,NK-92 cells contained higher level of PRL-R than YT cells,which correlated to the enhanced capacity of the cells to proliferate and to lyse target cells in response to PRL stimulation in the presence of trace amount of IL-2 or IL-15 in vitro. Two differences between IL-2 and IL-15 in functioning on human NK cells were for the first time observed. PRL synergized with IL-15 to improve proliferation of NK cells in a dose-dependent manner without double peak manifesting like IL-2. Although PRL enhanced the cytotoxicity of IL-2 or IL- 15 activated NK cells,it exerted the function through up-regulating gene expression of perforin without influence of FasL in IL-2-stimulated NK cells,while in IL-15-stimulated NK cells,PRL did the function through up-regulating gene expression of both perforin and FasL but not IFNγ. PRL increased expressions of IL-2Rα on membrane and of IL-2 mRNA in cells,indicating that PRL up-regulated NK cell function by improving positive feedback between IL-2 and IL-2R. The similar results were also observed in network between IL-15 and IL-15R. These data indicate a potential role of PRL in human NK cell modulation. 展开更多
关键词 prolactin receptor NK cell INTERLEUKIN-2 interleukin-15.
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Expression level of pre-B-cell leukemia transcription factor 2 (PBX2) as a prognostic marker for gingival squamous cell carcinoma 被引量:1
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作者 Ying QIU Zhu-ling WANG +4 位作者 Shu-qing JIN Yu-fei PU Satoru TOYOSAWA Katsuyuki AOZASA Eiichi MORII 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2012年第3期168-175,共8页
Objective: In this study, we investigated the interrelationship between clinicopathologic findings and pre-B-cell leukemia transcription factor 2 (PBX2) expression in gingival squamous cell carcinoma (GSCC). Meth... Objective: In this study, we investigated the interrelationship between clinicopathologic findings and pre-B-cell leukemia transcription factor 2 (PBX2) expression in gingival squamous cell carcinoma (GSCC). Methods: Expression level of PBX2 was immunohistochemically examined in 66 GSCC subjects (30 men and 36 women) with ages ranging from 42 to 85 (median 64.5) years, in which staining intensity in tumor cells was categorized as either weaker (level 1 ) or equal to/stronger (level 2) than that in the endothelial cells. Results: PBX2 expression is correlated with valosin-containing protein (VCP) expression. Univariate and multivariate analyses revealed a high level of PBX2 expression to be a poor prognosticator for disease-free survival (DFS) and overall survival (OS), and PBX2 expression was an independent prognostic factor for both DFS and OS in GSCC. Conclusions: PBX2 expression level in GSCC is proqnostic. PBX2 may be a useful marker to identify the potential for progression in GSCC. 展开更多
关键词 Gingival squamous cell carcinoma Pre-B-cell leukemia transcription factor 2 (PBX2 Prognosis
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