Isolation of a Genomic DNA for Gastrodia Antifungal Protein and Analyses of Its Promoter in Transgenic Tobacco

A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter activity, the 5'-flanking region - 1 157 lip upstream from the putative transcription start site was fused to the coding sequence of beta-glucuronidase (GUS) gene and transformed into Nicotiana tabacum. The strongest GUS activity was detected in the roots of transgenic tobacco, followed by stems. The leaves only showed a low GUS activity. Furthermore, the promoter established inducible expression pattern in transgenic tobacco upon fungus Trichoderma viride inoculation and jasmonic acid and salicylic acid treatments.

脚行众生相

王五金财轮子行当的人物,干搬运、当苦力,以来自农村的汉子居多。其中又以来自贫瘠山区的穷汉居多。比如我们家乡,早年来城里闯江湖吃脚行的就不少。先说一个最熟悉的:王五金财。

行走在两个气球间的女孩子

一从亮得晃眼的太阳底下走进吉盛伟邦,冷气嗖地裹住身体,杰克琳的抵抗情绪才消散。她迅速扫视着那些线条简洁却有一张张尊贵面孔的北欧家具,心里那张温馨的网渐渐地舒展。大罗马就像那些木质考究的家具花纹,矜持而不失亲切地对她说,快擦擦汗,一下子从高温里进到冷气中,小心着凉。杰克琳不情愿地从坤包里掏出纸巾,尝试着看了看大罗马,见西装革履的他没有举手代劳的意思,便自顾自地擦拭去脸上细密的汗珠,将纸巾狠狠地扔进废物桶,心里暗暗地骂了一句:伪君子。大罗马目视前方。