本研究采用国际标准PPD及国际标准结素试验技术,特别是结素试验操作人员和阅读结素反应人员均经过国际标准结素试验技术培训及考核合格的情况下,对100名五年级学生进行PPD结素试验,两名结素反应阅读者及记录者采取双盲法分别测量和记录...本研究采用国际标准PPD及国际标准结素试验技术,特别是结素试验操作人员和阅读结素反应人员均经过国际标准结素试验技术培训及考核合格的情况下,对100名五年级学生进行PPD结素试验,两名结素反应阅读者及记录者采取双盲法分别测量和记录每一个学生结素反应的平均直径和最大横径,然后,仍然采用双盲法进行第二次测量。运用计算机流行病学统计分析软件Epi Info 6反复比较分析最大横径测量法与平均直径测量法所测得的结素反应结果。研究结果表明:两名结素反应阅读者的个人技术具有稳定性,两人之间的阅读技术达到一致性,最大横径测量法与平均直径测量法测得的结果具有一致性和可比性。同时提示,培训在结素试验中起到至关重要的作用。展开更多
Objective:To investigate the binding characteri stics of endothelial cell (EC) with LPS free from the participation of serum fac tors. Methods:Laser confocal microscope was employed in the observat ion of the binding ...Objective:To investigate the binding characteri stics of endothelial cell (EC) with LPS free from the participation of serum fac tors. Methods:Laser confocal microscope was employed in the observat ion of the binding of EC with FITC-LPS. The KD and the binding sites of each EC were calculated by radioligand binding assay of receptors (RBA) using [ 3H] -LPS. Results:The binding of EC with LPS was saturable, time and con centration dependent and it could be competed with overdosed LPS of the same typ e. The fluorescence mainly distributed in cytoplasm, especially near the nucleus , which could also be stained. Conclusions:There might be some specific LPS binding sites exi sting on ECs and LPS could function intracellularily.展开更多
文摘本研究采用国际标准PPD及国际标准结素试验技术,特别是结素试验操作人员和阅读结素反应人员均经过国际标准结素试验技术培训及考核合格的情况下,对100名五年级学生进行PPD结素试验,两名结素反应阅读者及记录者采取双盲法分别测量和记录每一个学生结素反应的平均直径和最大横径,然后,仍然采用双盲法进行第二次测量。运用计算机流行病学统计分析软件Epi Info 6反复比较分析最大横径测量法与平均直径测量法所测得的结素反应结果。研究结果表明:两名结素反应阅读者的个人技术具有稳定性,两人之间的阅读技术达到一致性,最大横径测量法与平均直径测量法测得的结果具有一致性和可比性。同时提示,培训在结素试验中起到至关重要的作用。
文摘Objective:To investigate the binding characteri stics of endothelial cell (EC) with LPS free from the participation of serum fac tors. Methods:Laser confocal microscope was employed in the observat ion of the binding of EC with FITC-LPS. The KD and the binding sites of each EC were calculated by radioligand binding assay of receptors (RBA) using [ 3H] -LPS. Results:The binding of EC with LPS was saturable, time and con centration dependent and it could be competed with overdosed LPS of the same typ e. The fluorescence mainly distributed in cytoplasm, especially near the nucleus , which could also be stained. Conclusions:There might be some specific LPS binding sites exi sting on ECs and LPS could function intracellularily.
