AIM: To evaluate the potential association of xeroderma pigmentosum group D (XPD) codon 751 variant with outcome after chemo-radiotherapy in patients with resected gastric cancer. METHODS: We used PCR-RFLP to evaluate...AIM: To evaluate the potential association of xeroderma pigmentosum group D (XPD) codon 751 variant with outcome after chemo-radiotherapy in patients with resected gastric cancer. METHODS: We used PCR-RFLP to evaluate the genetic XPD Lys751Gln polymorphisms in 44 patients with stage Ⅲ (48%) and Ⅳ (20%) gastric cancer treated with surgery following radiation therapy plus 5-fluorouracil/ leucovorin based chemotherapy. RESULTS: Statistical analysis showed that 75% (12 of 16) of relapse patients showed Lys/Lys genotype more frequently (P = 0.042). The Lys polymorphism was an independent predictor of high-risk relapse-free survival from Cox analysis (HR: 3.07, 95% CI: 1.07-8.78, P = 0.036) and Kaplan-Meir test (P = 0.027, log-rank test). CONCLUSION: XPD Lys751Gln polymorphism may be an important marker in the prediction of clinical outcome to chemo-radiotherapy in resected gastric cancer patients.展开更多
Objective To explore the efficacy and safty of sorafenib in Child-Pugh class B to class C hepatocellular carcinoma （HCC）. Methods In this three-center open-label study from November 2011 to May 2013, we randomly as...Objective To explore the efficacy and safty of sorafenib in Child-Pugh class B to class C hepatocellular carcinoma （HCC）. Methods In this three-center open-label study from November 2011 to May 2013, we randomly assigned 189 patients with advanced Child-Pugh class B or C HCC patients into two groups, one group with 95 patient to receive sorafenib （400 mga time, twice a day） and the other group with 94 patients to receive best supportive care. The primary end points were progression-free survival and overall survival. Results The median progression-free survival was 2.2 months and 1.9 months in the sorafenib group and best supportive care group respectively （Hazard ratio in the sorafenib group, 0.55; 95% confidence interval, 0.40-0.75; P=O.O02）. The median overall survival was 4.0 months and 3.5 months in the sorafenib group and best supportive care group respectively （Hazard ratio in the sorafenib group, 0.48; 95% confidence interval, 0.35-0.68; P〈0.001）. The main adverse effect of sorafenib was rash and ache of the skin （in 51.7% patients）. The incidences of severe rash, diarrhea, and dry skin were 5.6%, 5.6%, and 2.2% in the sorafenib group. One patient reached partial response in the sorafenib group. Conclusions Sorafenib is safe in patients with liver function impaired advanced HCC. It is effective in terms of progression-free survival and overall survival compared with best supportive care. Liver functions are the important predictive factors.展开更多
Objective: The beneficial effect of percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI) has been well established, but there is the problem of no-reflow phenomenon which is an a...Objective: The beneficial effect of percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI) has been well established, but there is the problem of no-reflow phenomenon which is an adverse prognostic factor in primary PCI. In the present study the effect of a distal protection device (PercuSurge GuardWire; GW) on epicardial blood flow and myocardial perfusion was evaluated. Methods and Results: Patients with AMI were randomly divided into 2 groups, the GW and the control groups. The GW group included 52 patients with AMI who underwent primary PCI with GW protection and the control group included 60 patients who underwent primary PCI without GW protection. Epicardial blood flow in the infarct-related artery (IRA) and myocardial perfusion were evaluated according to the thrombolysis in myocardial infarction (TIMI) flow grade and the myocardial blush grade (MBG). We found TIMI score of 3 was obtained significantly more frequently in the GW group (96%) than in the control group (80%). The MBG score of 3 was obtained also significantly greater in the GW group (65%) than in the control group (33%). Conclusion: Primary PCI with GW protection can significantly improve epicardial blood flow and myocardial perfusion.展开更多
Nine cowsheds were used, the differences between each cowshed are orientation （east-west or north-south）, cooling （with or without）, and height （3 m, 5 m or 8 m height）. Dry-bulb temperature, relative humidity, ...Nine cowsheds were used, the differences between each cowshed are orientation （east-west or north-south）, cooling （with or without）, and height （3 m, 5 m or 8 m height）. Dry-bulb temperature, relative humidity, dew point, shaded area, air velocity, and maximum temperature were measured beneath each shed. The temperature-humidity index （THI） and the shading efficiency were calculated for each cowshed. The trials were conducted using 180 lactating Holstein Friesian cows; twenty cows housed beneath each shade structure. Measurements performed on cows were respiration rate, skin temperature, and rectal temperature; moreover, the milk produced by each cow was recorded, and the feed intake. High air velocities were recorded under high shade structures which enhance the aeration; consequently, maximum temperatures, and THI recorded beneath high sheds were less than those recorded beneath low sheds; also, skin temperatures, and respiration rates for cows housed under high sheds were less than those measured for animals housed under low sheds（differences were found to be significant）. Thereby, increasing shed height enhances dairy cows＇ microclimate under Egyptian conditions （hot climate）; consequently, the milk production increases. The best orientation is the east-west orientation, which is preferable for hot climates. The exploitation of cooling has significant effect on microclimate, cooling enhances the ambient environment and provides comfortable zone for dairy cows within which cows reach high production levels.展开更多
Stem cells are defined by their capacity of self-renewal and multilineage differentiation, which make them uniquely situated to treat a broad spectrum of human diseases. Based on a series of remarkable studies in seve...Stem cells are defined by their capacity of self-renewal and multilineage differentiation, which make them uniquely situated to treat a broad spectrum of human diseases. Based on a series of remarkable studies in several fields of regenerative medicine, their application is not too far from the clinical practice. Full-thickness burns and severe traumas can injure skin and its appendages, which protect animals from water loss. temperature change, radiation, trauma and infection. In adults, the normal outcome of repair of massive full-thickness burns is fibrosis and scarring without any appendages, such as hair follicles, sweat and sebaceous glands. Perfect skin regeneration has been considered im-possible due to the limited regenerative capacity of epider- mal keratinocytes, which are generally thought to be the key source of the epidermis and skin appendages. Currently, researches on stem cells, such as epidermal stem cells, dermal stem cells, mesenchymal stem cells from bone marrow, and embryonic stem cells, bring promise to functional repair of skin after severe burn injury, namely, complete regeneration of skin and its appendages. In this study, we present an overview of the most recent advances in skin repair and regeneration by using stem cells.展开更多
基金Supported by a grant from the Navarra Government 70/2004
文摘AIM: To evaluate the potential association of xeroderma pigmentosum group D (XPD) codon 751 variant with outcome after chemo-radiotherapy in patients with resected gastric cancer. METHODS: We used PCR-RFLP to evaluate the genetic XPD Lys751Gln polymorphisms in 44 patients with stage Ⅲ (48%) and Ⅳ (20%) gastric cancer treated with surgery following radiation therapy plus 5-fluorouracil/ leucovorin based chemotherapy. RESULTS: Statistical analysis showed that 75% (12 of 16) of relapse patients showed Lys/Lys genotype more frequently (P = 0.042). The Lys polymorphism was an independent predictor of high-risk relapse-free survival from Cox analysis (HR: 3.07, 95% CI: 1.07-8.78, P = 0.036) and Kaplan-Meir test (P = 0.027, log-rank test). CONCLUSION: XPD Lys751Gln polymorphism may be an important marker in the prediction of clinical outcome to chemo-radiotherapy in resected gastric cancer patients.
文摘Objective To explore the efficacy and safty of sorafenib in Child-Pugh class B to class C hepatocellular carcinoma （HCC）. Methods In this three-center open-label study from November 2011 to May 2013, we randomly assigned 189 patients with advanced Child-Pugh class B or C HCC patients into two groups, one group with 95 patient to receive sorafenib （400 mga time, twice a day） and the other group with 94 patients to receive best supportive care. The primary end points were progression-free survival and overall survival. Results The median progression-free survival was 2.2 months and 1.9 months in the sorafenib group and best supportive care group respectively （Hazard ratio in the sorafenib group, 0.55; 95% confidence interval, 0.40-0.75; P=O.O02）. The median overall survival was 4.0 months and 3.5 months in the sorafenib group and best supportive care group respectively （Hazard ratio in the sorafenib group, 0.48; 95% confidence interval, 0.35-0.68; P〈0.001）. The main adverse effect of sorafenib was rash and ache of the skin （in 51.7% patients）. The incidences of severe rash, diarrhea, and dry skin were 5.6%, 5.6%, and 2.2% in the sorafenib group. One patient reached partial response in the sorafenib group. Conclusions Sorafenib is safe in patients with liver function impaired advanced HCC. It is effective in terms of progression-free survival and overall survival compared with best supportive care. Liver functions are the important predictive factors.
文摘Objective: The beneficial effect of percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI) has been well established, but there is the problem of no-reflow phenomenon which is an adverse prognostic factor in primary PCI. In the present study the effect of a distal protection device (PercuSurge GuardWire; GW) on epicardial blood flow and myocardial perfusion was evaluated. Methods and Results: Patients with AMI were randomly divided into 2 groups, the GW and the control groups. The GW group included 52 patients with AMI who underwent primary PCI with GW protection and the control group included 60 patients who underwent primary PCI without GW protection. Epicardial blood flow in the infarct-related artery (IRA) and myocardial perfusion were evaluated according to the thrombolysis in myocardial infarction (TIMI) flow grade and the myocardial blush grade (MBG). We found TIMI score of 3 was obtained significantly more frequently in the GW group (96%) than in the control group (80%). The MBG score of 3 was obtained also significantly greater in the GW group (65%) than in the control group (33%). Conclusion: Primary PCI with GW protection can significantly improve epicardial blood flow and myocardial perfusion.
文摘Nine cowsheds were used, the differences between each cowshed are orientation （east-west or north-south）, cooling （with or without）, and height （3 m, 5 m or 8 m height）. Dry-bulb temperature, relative humidity, dew point, shaded area, air velocity, and maximum temperature were measured beneath each shed. The temperature-humidity index （THI） and the shading efficiency were calculated for each cowshed. The trials were conducted using 180 lactating Holstein Friesian cows; twenty cows housed beneath each shade structure. Measurements performed on cows were respiration rate, skin temperature, and rectal temperature; moreover, the milk produced by each cow was recorded, and the feed intake. High air velocities were recorded under high shade structures which enhance the aeration; consequently, maximum temperatures, and THI recorded beneath high sheds were less than those recorded beneath low sheds; also, skin temperatures, and respiration rates for cows housed under high sheds were less than those measured for animals housed under low sheds（differences were found to be significant）. Thereby, increasing shed height enhances dairy cows＇ microclimate under Egyptian conditions （hot climate）; consequently, the milk production increases. The best orientation is the east-west orientation, which is preferable for hot climates. The exploitation of cooling has significant effect on microclimate, cooling enhances the ambient environment and provides comfortable zone for dairy cows within which cows reach high production levels.
文摘Stem cells are defined by their capacity of self-renewal and multilineage differentiation, which make them uniquely situated to treat a broad spectrum of human diseases. Based on a series of remarkable studies in several fields of regenerative medicine, their application is not too far from the clinical practice. Full-thickness burns and severe traumas can injure skin and its appendages, which protect animals from water loss. temperature change, radiation, trauma and infection. In adults, the normal outcome of repair of massive full-thickness burns is fibrosis and scarring without any appendages, such as hair follicles, sweat and sebaceous glands. Perfect skin regeneration has been considered im-possible due to the limited regenerative capacity of epider- mal keratinocytes, which are generally thought to be the key source of the epidermis and skin appendages. Currently, researches on stem cells, such as epidermal stem cells, dermal stem cells, mesenchymal stem cells from bone marrow, and embryonic stem cells, bring promise to functional repair of skin after severe burn injury, namely, complete regeneration of skin and its appendages. In this study, we present an overview of the most recent advances in skin repair and regeneration by using stem cells.
