[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with...[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with PCV2 in vivo. [Method] Healthy 40-day-old Landrace piglets were infected with porcine circovirus type 2 （PCV2） and euthanized on the 34, 7rd, 14th, 21st and 35th d post inoculation （DPI）. The porcine skin-derived dendritic cells （DCs） were collected to analyze the transcrip- tional levels of molecules （LMP7, UBP, MHC-I, calreticulin） associated with endogenous antigen processing and presentation by using real-time fluorescent quantitative PCR （real-time FQ-PCR）. [Result] The results showed that the level of LMP7 mR- NAs was reduced significantly on the 3DPI （P〈0.05）; the level of UBP mRNAs was consistently up-regulated, which increased significantly on the 21DPI and 35DPI （P〈 0.05）; the level of MHC-I mRNAs was significantly down-regulated on the 7DPI （P〈 0.05）; the level of calreticulin mRNAs was up-regulated slightly without significant dif- ference. [Conclusion] PCV2 can inhibit the endogenous antigen processing and presentation ability of porcine skin-derived DCs at early stages of infection.展开更多
Objective:To investigate the culture method of skin-derived precursors (SKPs) and to explore a new cell source for cell transp lantation of central nervous system. Methods:Cells from skins of juvenile and adult mice w...Objective:To investigate the culture method of skin-derived precursors (SKPs) and to explore a new cell source for cell transp lantation of central nervous system. Methods:Cells from skins of juvenile and adult mice were iso lated and cultured in serum-free medium. A mechanical method was chosen to pass age these cells and they were identified by the immunocytochemistry assay. Results:SKPs could be isolated from adult and neonatal skins . They could be maintained in vitro for long periods with stable proliferation, and expanded as undifferentiated cells in culture for more than 12 passages. Abo ut 50% of SKPs expressed nestin and majority of these cells expressed fibronecti n when they were plated on polyornithine and laminin coated plates. About 5% cel ls showed neuronal differentiation and expressed neurofilament-M (NF-M) and NS E when SKPs were plated in serum-containing medium, and these cells could also differentiate into adipocytes and fibroblast-like cells. Conclusions:The data support the hypothesis that adult skin contains stem cells capable of differentiating into neurons, adipocytes, and fib roblast-like cells. They may represent an alternative autologous stem cell sour ce for CNS cell transplantation.展开更多
基金Supported by Natural Science Foundation of Beijing "Effect of porcine skin-derived dendritic cells on PCV infection" (6062006)Beijing Organization Department Project"Influence of PCV infection on bone marrow cell differentiation" (20061D0502100282)~~
文摘[Objective] This study aimed to investigate the changes of the transcriptional levels of molecules associated with endogenous antigen processing and presenta- tion in porcine skin-derived dendritic cells infected with PCV2 in vivo. [Method] Healthy 40-day-old Landrace piglets were infected with porcine circovirus type 2 （PCV2） and euthanized on the 34, 7rd, 14th, 21st and 35th d post inoculation （DPI）. The porcine skin-derived dendritic cells （DCs） were collected to analyze the transcrip- tional levels of molecules （LMP7, UBP, MHC-I, calreticulin） associated with endogenous antigen processing and presentation by using real-time fluorescent quantitative PCR （real-time FQ-PCR）. [Result] The results showed that the level of LMP7 mR- NAs was reduced significantly on the 3DPI （P〈0.05）; the level of UBP mRNAs was consistently up-regulated, which increased significantly on the 21DPI and 35DPI （P〈 0.05）; the level of MHC-I mRNAs was significantly down-regulated on the 7DPI （P〈 0.05）; the level of calreticulin mRNAs was up-regulated slightly without significant dif- ference. [Conclusion] PCV2 can inhibit the endogenous antigen processing and presentation ability of porcine skin-derived DCs at early stages of infection.
基金ThisworkwassupportedbyNaturalScienceFoundationofGuangdongProvince (No .01245200001)andtheNationalNaturalScienceFoundationofChina(KeyProjectNo .3 9993 4 3 0 )
文摘Objective:To investigate the culture method of skin-derived precursors (SKPs) and to explore a new cell source for cell transp lantation of central nervous system. Methods:Cells from skins of juvenile and adult mice were iso lated and cultured in serum-free medium. A mechanical method was chosen to pass age these cells and they were identified by the immunocytochemistry assay. Results:SKPs could be isolated from adult and neonatal skins . They could be maintained in vitro for long periods with stable proliferation, and expanded as undifferentiated cells in culture for more than 12 passages. Abo ut 50% of SKPs expressed nestin and majority of these cells expressed fibronecti n when they were plated on polyornithine and laminin coated plates. About 5% cel ls showed neuronal differentiation and expressed neurofilament-M (NF-M) and NS E when SKPs were plated in serum-containing medium, and these cells could also differentiate into adipocytes and fibroblast-like cells. Conclusions:The data support the hypothesis that adult skin contains stem cells capable of differentiating into neurons, adipocytes, and fib roblast-like cells. They may represent an alternative autologous stem cell sour ce for CNS cell transplantation.
