慢性创面是全球医疗保健系统的社会负担,也是患者的经济负担,严重影响了他们的生活质量。老年人和糖尿病患者等易感人群的迅速增长,使慢性创面的发生率一直在稳步上升。慢性创面的特点是伤口愈合过程延迟,在标准护理下愈合比急性(即健康...慢性创面是全球医疗保健系统的社会负担,也是患者的经济负担,严重影响了他们的生活质量。老年人和糖尿病患者等易感人群的迅速增长,使慢性创面的发生率一直在稳步上升。慢性创面的特点是伤口愈合过程延迟,在标准护理下愈合比急性(即健康)创面需要更长的时间。由于感染的参与和严重程度,慢性创面的愈合一直是令人担忧的问题。生物材料在慢性创面管理中的功能和作用已经得到普遍认可,这种生物材料之一是胶原蛋白,胶原蛋白作为伤口敷料中最常用的材料之一,因为它有助于模仿本地伤口微环境,它被认为是大多数用于创面愈合配方的关键成分。传统的胶原蛋白从陆地动物中提取出来,它存在宗教限制,也存在疾病传播的风险;而鱼皮胶原蛋白从海洋生物中提取出来,因其具有独特的结构和功能特性,使其成为细胞附着、生长和分化的天然基质。鱼皮胶原蛋白不仅可以促进皮肤上皮化、血管化、成纤维细胞迁移,还能整体加快创面愈合的能力,未来将成为一种潜在的更有前途的新型材料分子。本文我们介绍了用于慢性创面愈合的鱼皮胶原蛋白的最新研究进展。旨在为进一步研究提供新的观点。Chronic wounds are a social burden on the global healthcare system and an economic burden on patients, seriously affecting their quality of life. The rapid growth of susceptible populations such as the elderly and diabetes patients has led to a steady increase in the incidence of chronic wounds. The characteristic of chronic wounds is delayed wound healing process, which takes longer to heal under standard care than acute (i.e. healthy) wounds. The healing of chronic wounds has always been a concern due to the involvement and severity of infections. The function and role of biomaterials in chronic wound management have been widely recognized. One of these biomaterials is collagen, which is one of the most commonly used materials in wound dressings because it helps mimic the local wound microenvironment. It is considered a key ingredient in most wound healing formulas. Traditional collagen is extracted from terrestrial animals, which has religious restrictions and the risk of disease transmission;Fish skin collagen is extracted from marine organisms and has unique structural and functional properties, making it a natural matrix for cell attachment, growth, and differentiation. Fish skin collagen can not only promote skin epithelialization, vascularization, and fibroblast migration, but also accelerate the overall ability of wound healing. In the future, it will become a potential and more promising new material molecule. In this article, we introduce the latest research progress on fish skin collagen used for chronic wound healing. Intended to provide new perspectives for further research.展开更多
目的:研究黑线鳕鱼(Melanogrammus aeglefinus)皮胶原蛋白胰蛋白酶酶解多肽的抗氧化、抑制光损伤作用,提高鱼皮的再利用价值。方法:利用单因素试验和响应面试验设计,探讨黑线鳕鱼皮胶原蛋白的最佳酶解工艺。将制得的酶解多肽通过基质辅...目的:研究黑线鳕鱼(Melanogrammus aeglefinus)皮胶原蛋白胰蛋白酶酶解多肽的抗氧化、抑制光损伤作用,提高鱼皮的再利用价值。方法:利用单因素试验和响应面试验设计,探讨黑线鳕鱼皮胶原蛋白的最佳酶解工艺。将制得的酶解多肽通过基质辅助激光解离飞行时间串联质谱仪(matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry,MALDI-TOF MS/MS)测定多肽序列。利用Discovery Studio中CDOCKER模块将所得的优势肽与抗氧化相关的Kelch样环氧氯丙烷相关蛋白-1(Keap1)进行分子对接。用不同质量浓度的多肽预处理人表皮角质形成细胞(Ha Ca T)后,使用中波紫外线诱导HaCaT细胞构建光损伤模型,通过超氧化歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、丙二醛(malondialdehyde,MDA)含量及细胞凋亡与周期来研究酶解多肽抑制光损伤的能力。结果:黑线鳕鱼皮胶原蛋白在胰蛋白酶质量分数1.23%、酶解温度52.95℃、酶解时间1.75 h条件下,水解度为78.33%。MALDI-TOF MS/MS测定出酶解液中的优势肽序列为V-IFFVTMGTP-R、L-SIVPV-R和L-MHT-T。通过分子对接发现V-IFFVTMGTP-R与Keap1结合最稳定,从而预测其具有抗氧化的作用。用250 mg/m L酶解液处理光损伤的HaCaT细胞,其SOD、GSHPx活性较模型组极显著提高(P<0.01),MDA含量极显著降低(P<0.01)。结论:黑线鳕鱼皮胶原蛋白胰蛋白酶酶解多肽具有抗氧化活性,对光损伤具有一定的抑制作用。展开更多
文摘慢性创面是全球医疗保健系统的社会负担,也是患者的经济负担,严重影响了他们的生活质量。老年人和糖尿病患者等易感人群的迅速增长,使慢性创面的发生率一直在稳步上升。慢性创面的特点是伤口愈合过程延迟,在标准护理下愈合比急性(即健康)创面需要更长的时间。由于感染的参与和严重程度,慢性创面的愈合一直是令人担忧的问题。生物材料在慢性创面管理中的功能和作用已经得到普遍认可,这种生物材料之一是胶原蛋白,胶原蛋白作为伤口敷料中最常用的材料之一,因为它有助于模仿本地伤口微环境,它被认为是大多数用于创面愈合配方的关键成分。传统的胶原蛋白从陆地动物中提取出来,它存在宗教限制,也存在疾病传播的风险;而鱼皮胶原蛋白从海洋生物中提取出来,因其具有独特的结构和功能特性,使其成为细胞附着、生长和分化的天然基质。鱼皮胶原蛋白不仅可以促进皮肤上皮化、血管化、成纤维细胞迁移,还能整体加快创面愈合的能力,未来将成为一种潜在的更有前途的新型材料分子。本文我们介绍了用于慢性创面愈合的鱼皮胶原蛋白的最新研究进展。旨在为进一步研究提供新的观点。Chronic wounds are a social burden on the global healthcare system and an economic burden on patients, seriously affecting their quality of life. The rapid growth of susceptible populations such as the elderly and diabetes patients has led to a steady increase in the incidence of chronic wounds. The characteristic of chronic wounds is delayed wound healing process, which takes longer to heal under standard care than acute (i.e. healthy) wounds. The healing of chronic wounds has always been a concern due to the involvement and severity of infections. The function and role of biomaterials in chronic wound management have been widely recognized. One of these biomaterials is collagen, which is one of the most commonly used materials in wound dressings because it helps mimic the local wound microenvironment. It is considered a key ingredient in most wound healing formulas. Traditional collagen is extracted from terrestrial animals, which has religious restrictions and the risk of disease transmission;Fish skin collagen is extracted from marine organisms and has unique structural and functional properties, making it a natural matrix for cell attachment, growth, and differentiation. Fish skin collagen can not only promote skin epithelialization, vascularization, and fibroblast migration, but also accelerate the overall ability of wound healing. In the future, it will become a potential and more promising new material molecule. In this article, we introduce the latest research progress on fish skin collagen used for chronic wound healing. Intended to provide new perspectives for further research.
文摘目的:研究黑线鳕鱼(Melanogrammus aeglefinus)皮胶原蛋白胰蛋白酶酶解多肽的抗氧化、抑制光损伤作用,提高鱼皮的再利用价值。方法:利用单因素试验和响应面试验设计,探讨黑线鳕鱼皮胶原蛋白的最佳酶解工艺。将制得的酶解多肽通过基质辅助激光解离飞行时间串联质谱仪(matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry,MALDI-TOF MS/MS)测定多肽序列。利用Discovery Studio中CDOCKER模块将所得的优势肽与抗氧化相关的Kelch样环氧氯丙烷相关蛋白-1(Keap1)进行分子对接。用不同质量浓度的多肽预处理人表皮角质形成细胞(Ha Ca T)后,使用中波紫外线诱导HaCaT细胞构建光损伤模型,通过超氧化歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、丙二醛(malondialdehyde,MDA)含量及细胞凋亡与周期来研究酶解多肽抑制光损伤的能力。结果:黑线鳕鱼皮胶原蛋白在胰蛋白酶质量分数1.23%、酶解温度52.95℃、酶解时间1.75 h条件下,水解度为78.33%。MALDI-TOF MS/MS测定出酶解液中的优势肽序列为V-IFFVTMGTP-R、L-SIVPV-R和L-MHT-T。通过分子对接发现V-IFFVTMGTP-R与Keap1结合最稳定,从而预测其具有抗氧化的作用。用250 mg/m L酶解液处理光损伤的HaCaT细胞,其SOD、GSHPx活性较模型组极显著提高(P<0.01),MDA含量极显著降低(P<0.01)。结论:黑线鳕鱼皮胶原蛋白胰蛋白酶酶解多肽具有抗氧化活性,对光损伤具有一定的抑制作用。
