Fungus Rhizopus oryzae has a probiotic potential, but the potency is not yet explored. Probiotics are usually used as feed additive, including to the chicken. Probiotic needs a suitable medium for their life. The aim ...Fungus Rhizopus oryzae has a probiotic potential, but the potency is not yet explored. Probiotics are usually used as feed additive, including to the chicken. Probiotic needs a suitable medium for their life. The aim of the study was to know which preparations can produce suitable medium for the growth of Rhizopus oryzae on the dried culture formed. The study used two preparations. Preparation 1 （a） consisted of rice bran （1 kg） and culture ofRhizopus oryzae on Potato Dextrose Broth （PDB medium） （500 mL）; then preparation 1 （b） consisted of corn grain （1 kg） and culture of Rhizopus oryzae on PDB medium （500 mL）. Preparation 2 （a） consisted of rice bran （1 kg）, culture of Rhizopus oryzae on Potato Dextrose Agar （PDA medium） （seven petri dishes） and was added with sterile distilled water （500 mL）; preparation 2 （b） consisted of corn grain （1 kg）, culture of Rhizopus oryzae on PDA medium （seven petri dishes） and was added with sterile distilled water （500 mL）. The culture of Rhizopus oryzae even both on PDB and PDA were 3 d of incubation. All preparations were incubated for 7 d then they were dried under the sun. Parameter evaluation was the growth performance of the fungus in the dried culture media. The growth performance was analyzed by microscopic analysis and the number of colony of the fungi in the two dried culture media. The results showed that from the macroscopic analysis, the sample of both dried culture media which were placed on PDA medium showed the growth of mycelia and spores of the same kind of fungus and it was proved by microscopic observation that the fruiting bodies （mycelia and spores） of that ftmgus was Rhizopus oryzae. The colonies number of the fungi from preparation 1 （a） and 1 （b） were 0,4 × 10 cfu/gr and 2.4 × 10^3 cfu/gr, respectively, and from preparation 2 （a） and 2 （b） were 1.3 × 10^5 cfu/gr and 8.3 ×10^2 cfu/gr, respectively. It could be concluded that the product of the dried culture of probiotic Rizopus oryzae from the preparation 2 with rice brand or corn grain mediums can be used as feed additive to indigenous chicken.展开更多
文摘Fungus Rhizopus oryzae has a probiotic potential, but the potency is not yet explored. Probiotics are usually used as feed additive, including to the chicken. Probiotic needs a suitable medium for their life. The aim of the study was to know which preparations can produce suitable medium for the growth of Rhizopus oryzae on the dried culture formed. The study used two preparations. Preparation 1 （a） consisted of rice bran （1 kg） and culture ofRhizopus oryzae on Potato Dextrose Broth （PDB medium） （500 mL）; then preparation 1 （b） consisted of corn grain （1 kg） and culture of Rhizopus oryzae on PDB medium （500 mL）. Preparation 2 （a） consisted of rice bran （1 kg）, culture of Rhizopus oryzae on Potato Dextrose Agar （PDA medium） （seven petri dishes） and was added with sterile distilled water （500 mL）; preparation 2 （b） consisted of corn grain （1 kg）, culture of Rhizopus oryzae on PDA medium （seven petri dishes） and was added with sterile distilled water （500 mL）. The culture of Rhizopus oryzae even both on PDB and PDA were 3 d of incubation. All preparations were incubated for 7 d then they were dried under the sun. Parameter evaluation was the growth performance of the fungus in the dried culture media. The growth performance was analyzed by microscopic analysis and the number of colony of the fungi in the two dried culture media. The results showed that from the macroscopic analysis, the sample of both dried culture media which were placed on PDA medium showed the growth of mycelia and spores of the same kind of fungus and it was proved by microscopic observation that the fruiting bodies （mycelia and spores） of that ftmgus was Rhizopus oryzae. The colonies number of the fungi from preparation 1 （a） and 1 （b） were 0,4 × 10 cfu/gr and 2.4 × 10^3 cfu/gr, respectively, and from preparation 2 （a） and 2 （b） were 1.3 × 10^5 cfu/gr and 8.3 ×10^2 cfu/gr, respectively. It could be concluded that the product of the dried culture of probiotic Rizopus oryzae from the preparation 2 with rice brand or corn grain mediums can be used as feed additive to indigenous chicken.
