This study examined the effect of salinity on the expression of Na+/K+-ATPase(NKA) α-subunit and vacuolar-type H+-ATPase(V-ATPase) β-subunit gene in the gill of Litopenaeus vannamei. Semi-quantitative reverse transc...This study examined the effect of salinity on the expression of Na+/K+-ATPase(NKA) α-subunit and vacuolar-type H+-ATPase(V-ATPase) β-subunit gene in the gill of Litopenaeus vannamei. Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) assay showed that the expression of NKA α-subunit and V-ATPase β-subunit gene was significantly influenced by salinity. It was found that the NKA activity significantly varied with salinity in time and dose dependent manner; whereas the V-ATPase activity did not. The abundance of NKA α-subunit gene transcript increased rapidly when the salinity decreased from 26 b to 21, and slowly when the salinity decreased from 26 to 31 within the first 24 h. When the salinity decreased from 26 to 21, the transcription of NKA α-subunit gene in gill epithelium was higher at 12 h than that at 0 h, which was consistent with the result of immunoblotting assay of NKA α-subunit. In addition, salinity had a significant time- and dose-dependent effect on the concentration of biogenic amines in both hemolymph and gill. As compared to other parameters, the concentration of dopamine(DA) and 5-hydroxytryptamine(5-HT) varied in different patterns when the salinity decreased from 26 to 21 or increased from 26 to 31, suggesting that DA and 5-HT played different regulatory roles in osmotic adaption and modulation of shrimp when salinity varies.展开更多
Physico-chemical and biological data on water and sediments were collected from Mandovi and Zuari Rivers of Goa along the central west coast of India from 2007 to 2008 to understand the impact of developmental activit...Physico-chemical and biological data on water and sediments were collected from Mandovi and Zuari Rivers of Goa along the central west coast of India from 2007 to 2008 to understand the impact of developmental activities on the riverine environments. Factor analysis of the data segregated over high and low tides indicated a total of 6 factors each explaining 91% of variance during high tide and 84% of variance during low tide in Mandovi River. In Zuari River, a total of 6 factors explained 83% of variance during high tide and 4 factors explained 78% of variance during low tide. Factor analyses showed the dominance of nitrite and nitrate in Mandovi and of lead and mercury in Zuari. Higher inputs of anthropogenic ammonia and phenols associated with some phosphate were found in both these rivers. Water quality index （WQI） studies showed increasing overall index of pollution （OIP） values especially in the upstream of Zuari River, suggesting slightly polluted water. Increasing developmental activities along the banks of these rivers introduce domestic and anthropogenic nutrients and trace metals in their estuarine region, which flush out to the sea as per tidal currents, which otherwise can hamper the natural environments of these two rivers.展开更多
基金supported by the National Natural Sciences Foundation of China (31072193)the Scientific Research Foundation for Outstanding Young Scientists of Shandong Province (2006BS07005)
文摘This study examined the effect of salinity on the expression of Na+/K+-ATPase(NKA) α-subunit and vacuolar-type H+-ATPase(V-ATPase) β-subunit gene in the gill of Litopenaeus vannamei. Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) assay showed that the expression of NKA α-subunit and V-ATPase β-subunit gene was significantly influenced by salinity. It was found that the NKA activity significantly varied with salinity in time and dose dependent manner; whereas the V-ATPase activity did not. The abundance of NKA α-subunit gene transcript increased rapidly when the salinity decreased from 26 b to 21, and slowly when the salinity decreased from 26 to 31 within the first 24 h. When the salinity decreased from 26 to 21, the transcription of NKA α-subunit gene in gill epithelium was higher at 12 h than that at 0 h, which was consistent with the result of immunoblotting assay of NKA α-subunit. In addition, salinity had a significant time- and dose-dependent effect on the concentration of biogenic amines in both hemolymph and gill. As compared to other parameters, the concentration of dopamine(DA) and 5-hydroxytryptamine(5-HT) varied in different patterns when the salinity decreased from 26 to 21 or increased from 26 to 31, suggesting that DA and 5-HT played different regulatory roles in osmotic adaption and modulation of shrimp when salinity varies.
文摘Physico-chemical and biological data on water and sediments were collected from Mandovi and Zuari Rivers of Goa along the central west coast of India from 2007 to 2008 to understand the impact of developmental activities on the riverine environments. Factor analysis of the data segregated over high and low tides indicated a total of 6 factors each explaining 91% of variance during high tide and 84% of variance during low tide in Mandovi River. In Zuari River, a total of 6 factors explained 83% of variance during high tide and 4 factors explained 78% of variance during low tide. Factor analyses showed the dominance of nitrite and nitrate in Mandovi and of lead and mercury in Zuari. Higher inputs of anthropogenic ammonia and phenols associated with some phosphate were found in both these rivers. Water quality index （WQI） studies showed increasing overall index of pollution （OIP） values especially in the upstream of Zuari River, suggesting slightly polluted water. Increasing developmental activities along the banks of these rivers introduce domestic and anthropogenic nutrients and trace metals in their estuarine region, which flush out to the sea as per tidal currents, which otherwise can hamper the natural environments of these two rivers.
