Analysis of the gene expression differentiation in leaves of wheat （Triticum aestivum L.） cultivar Baofeng 7228, under salt stress, was carried out by Differential-Display Reverse Transcription-polymerase Chain Reac...Analysis of the gene expression differentiation in leaves of wheat （Triticum aestivum L.） cultivar Baofeng 7228, under salt stress, was carried out by Differential-Display Reverse Transcription-polymerase Chain Reaction （DDRT-PCR.） Twenty-seven differential cDNA fragments were obtained. The expression of the SR07 fragment was induced noticeably by salt treatment, and the nucleotide sequence homology of 87% between the SR07 fragment and PIPs （water channel proteins） was observed. Further research showed that a 561 bp open read frame was present in the SR07 fragment. Plant expression vector of pCAMBIA-SR07 was constructed and three transformants of tobacco （Nicotiana tobacum） mediated by Agrobacterium tumefaciens plasmid were obtained. Resistance to salt, PEG, and mannitol stresses of the three transformants were examined. No significant difference （P 〉 0.05） was observed between the control and the transformants in resistance to salt stress, but there was significant difference （P 〈 0.05） between the control and the transformants in resistance to PEG and mannitol stresses. Therefore, the expression of the SR07 fragment may play an important role in the water regulation of the plant.展开更多
[ Objective] The study aimed to reveal the effects of hypergravity on salt tolerance of wheat seedlings. [ Method ] The seed germination rate （Gr） and germination index （Gi） of wheat seedlings were measured under ...[ Objective] The study aimed to reveal the effects of hypergravity on salt tolerance of wheat seedlings. [ Method ] The seed germination rate （Gr） and germination index （Gi） of wheat seedlings were measured under hypergravity of 600 × g or 1 000 × g for 4 h and under the stress of 0.4% or 0.9% salt solution. The catalase （CAT） activity and malondialdehyde （MDA） content of wheat seedlings were also measured under 0.4% salt stress. [Result] Compared with seedlings in CK group （no hypergravity or salt stress）, the Gr and Gi of the seedlings in salt stress treatment decreased to different extents; while the Gr and Gi of the seedlings in treatment group （hypergravity and salt stress） increased compared with that in salt stress group. CAT in seedlings of hypergravity treatment was higher than that of CK group and 0.4% salt treatment group, meanwhile the MDA showed an opposite result. [ Conclusion] Hypergravity could enhance the salt resistance of wheat in specific range, and hypergravity of 600 × g for 4 h performed better than that of 1 000 ×g for 4 h.展开更多
Arbuscular mycorrhizal fungi(AMF)enhance plant tolerance to abiotic stresses like salinity and improve crop yield.However,their effects are variable,and the underlying cause of such variation remains largely unknown.T...Arbuscular mycorrhizal fungi(AMF)enhance plant tolerance to abiotic stresses like salinity and improve crop yield.However,their effects are variable,and the underlying cause of such variation remains largely unknown.This study aimed to assess how drought modifed the effect of AMF on plant resistance to high calcium-saline stress.A pot experiment was performed to examine how AMF inoculation affects the growth,photosynthetic activity,nutrient uptake and carbon(C),nitrogen(N)and phosphorus(P)stoichiometric ratio(C:N:P)of maize under high calcium stress and contrasting water conditions.The results showed that high calcium stress signifcantly reduced mycorrhizal colonization,biomass accumulation,C assimilation rate and C:N stoichiometric ratio in plant tissues.Besides,the adverse effects of calcium stress on photosynthesis were exacerbated under drought.AMF inoculation profoundly alleviated such reductions under drought and saline stress.However,it barely affected maize performance when subjected to calcium stress under well-watered conditions.Moreover,watering changed AMF impact on nutrient allocation in plant tissues.Under well-watered conditions,AMF stimulated P accumulation in roots and plant growth,but did not induce leaf P accumulation proportional to C and N,resulting in increased leaf C:P and N:P ratios under high calcium stress.In contrast,AMF decreased N content and the N:P ratio in leaves under drought.Overall,AMF inoculation improved maize resistance to calcium-salt stress through enhanced photosynthesis and modulation of nutrient stoichiometry,particularly under water defcit conditions.These results highlighted the regulatory role of AMF in carbon assimilation and nutrient homeostasis under compound stresses,and provide signifcant guidance on the improvement of crop yield in saline and arid regions.展开更多
基金This work was supported by the Foundation of Technological Department of Shaanxi Province (No. 06JK267)Basic Research Foundation of Xi’an University of Architecture & Technology (No. JC0507)
文摘Analysis of the gene expression differentiation in leaves of wheat （Triticum aestivum L.） cultivar Baofeng 7228, under salt stress, was carried out by Differential-Display Reverse Transcription-polymerase Chain Reaction （DDRT-PCR.） Twenty-seven differential cDNA fragments were obtained. The expression of the SR07 fragment was induced noticeably by salt treatment, and the nucleotide sequence homology of 87% between the SR07 fragment and PIPs （water channel proteins） was observed. Further research showed that a 561 bp open read frame was present in the SR07 fragment. Plant expression vector of pCAMBIA-SR07 was constructed and three transformants of tobacco （Nicotiana tobacum） mediated by Agrobacterium tumefaciens plasmid were obtained. Resistance to salt, PEG, and mannitol stresses of the three transformants were examined. No significant difference （P 〉 0.05） was observed between the control and the transformants in resistance to salt stress, but there was significant difference （P 〈 0.05） between the control and the transformants in resistance to PEG and mannitol stresses. Therefore, the expression of the SR07 fragment may play an important role in the water regulation of the plant.
基金the Start-up Research Grant of Excellent Talent in ZhongKai University of Agriculture and Engineering (G2360250)Shanxi Natural Science Foundation (20031067)~~
文摘[ Objective] The study aimed to reveal the effects of hypergravity on salt tolerance of wheat seedlings. [ Method ] The seed germination rate （Gr） and germination index （Gi） of wheat seedlings were measured under hypergravity of 600 × g or 1 000 × g for 4 h and under the stress of 0.4% or 0.9% salt solution. The catalase （CAT） activity and malondialdehyde （MDA） content of wheat seedlings were also measured under 0.4% salt stress. [Result] Compared with seedlings in CK group （no hypergravity or salt stress）, the Gr and Gi of the seedlings in salt stress treatment decreased to different extents; while the Gr and Gi of the seedlings in treatment group （hypergravity and salt stress） increased compared with that in salt stress group. CAT in seedlings of hypergravity treatment was higher than that of CK group and 0.4% salt treatment group, meanwhile the MDA showed an opposite result. [ Conclusion] Hypergravity could enhance the salt resistance of wheat in specific range, and hypergravity of 600 × g for 4 h performed better than that of 1 000 ×g for 4 h.
基金supported by China Postdoctoral Science Foundation(2021M703137)Chongqing Postdoctoral Science Foundation(cstc2021jcyj-bshX0195)+2 种基金Postdoctoral Foundation of Jiangsu Province of China(1501014B)Education Department of Sichuan Province(17ZB0211),the Ecological Security and Protection Key Laboratory of Sichuan Province(07144812)the Scientifc Research Foundation of Chongqing University of Technology(2021ZDZ022).
文摘Arbuscular mycorrhizal fungi(AMF)enhance plant tolerance to abiotic stresses like salinity and improve crop yield.However,their effects are variable,and the underlying cause of such variation remains largely unknown.This study aimed to assess how drought modifed the effect of AMF on plant resistance to high calcium-saline stress.A pot experiment was performed to examine how AMF inoculation affects the growth,photosynthetic activity,nutrient uptake and carbon(C),nitrogen(N)and phosphorus(P)stoichiometric ratio(C:N:P)of maize under high calcium stress and contrasting water conditions.The results showed that high calcium stress signifcantly reduced mycorrhizal colonization,biomass accumulation,C assimilation rate and C:N stoichiometric ratio in plant tissues.Besides,the adverse effects of calcium stress on photosynthesis were exacerbated under drought.AMF inoculation profoundly alleviated such reductions under drought and saline stress.However,it barely affected maize performance when subjected to calcium stress under well-watered conditions.Moreover,watering changed AMF impact on nutrient allocation in plant tissues.Under well-watered conditions,AMF stimulated P accumulation in roots and plant growth,but did not induce leaf P accumulation proportional to C and N,resulting in increased leaf C:P and N:P ratios under high calcium stress.In contrast,AMF decreased N content and the N:P ratio in leaves under drought.Overall,AMF inoculation improved maize resistance to calcium-salt stress through enhanced photosynthesis and modulation of nutrient stoichiometry,particularly under water defcit conditions.These results highlighted the regulatory role of AMF in carbon assimilation and nutrient homeostasis under compound stresses,and provide signifcant guidance on the improvement of crop yield in saline and arid regions.
