桑蚕滞育性卵盐酸刺激活化机理研究:Ⅲ、浸酸中无机离子渗入卵内及其对滞育性的影响

用原子吸收光谱法测出,桑蚕滞育性卵在盐酸浸渍过程中,卵壳上的铁、钙、镁、锌、铜等无机成份被溶解,并能随盐酸中的 K^+、Na^+、Mg^(2+)等无机离子一同渗入卵内。向桑蚕滞育性卵内注入微量无机离子的氯盐,结果 KCl、NaCl 以及 MgCl_2都能促进滞育性胚活化,而 CaCl_2、FeCl_3等则无此作用。说明浸酸过程中渗入卵内的 K^+、Na^+及 Mg^(2+)等起着促进滞育性胚活化的作用,这可能是滞育性卵盐酸刺激活化的重要原因之一。

家蚕冷藏浸酸蚕种的盐酸刺激量承受力试验

为了研究家蚕冷藏浸酸蚕种对盐酸刺激量的承受能力,以液温47.8℃、比重1.093、内含2%甲醛、浸渍6.5 min的盐酸刺激量为对照,以明虎A系和B系为供试品种,设置49.0、50.2和51.4℃3种盐酸液温,1.096、1.099和1.102共3种盐酸比重,9.0、11.5和14.0 min 3个盐酸浸渍时间,4%、6%和8%3种酸液中的甲醛浓度,分别比较了不同处理对冷藏浸酸蚕卵实用孵化率的影响。结果显示:蚕品种(系)间对盐酸刺激量的适应范围有较大差异,但整体倾向于适宜刺激量大于上述对照刺激量。蚕种对盐酸温度的变化最敏感,当盐酸液温51.4℃时,蚕卵表现出死卵和迟发育卵增加等发育不良影响;而甲醛浓度对蚕卵孵化的影响微弱。在现行47.8℃盐酸液温条件下,盐酸比重增至1.099或盐酸浸渍时间延长至11.5 min(明虎B系),能够显著提高调查品种的实用孵化率,降低催青过程中的死卵发生率。

The Study of Dipivefrin Hydrochloride Ophthalmic Gel

Dipivefrin hydrochloride ophthalmic gel was prepared and the release test and the isolated cornea permeation test of the formulation in vitro were investigated. The release test of the formulation was studied by using permeable membrane. The content and the release amount of Dipivefrin hydrochloride from the gel base were measured by high performance liquid chromatography. The cornea permeation test of the formulation was studied by using isolated rabbit corneas. The formulation release behavior in vitro followed the first-order kinetic equation. The release amount of Dipivefrin hydrochloride raised significantly with less polymer in the formulation. The cornea permeation behavior of the drug in vitro followed the first-order kinetic equation. The eye irritancy of Dipivefrin hydrochloride gel is lower than that of eyedrops.

不同保护时间对家蚕即时浸酸种一日孵化率的影响

家蚕蛾产卵后保护15～20h(25℃)的蚕卵,浸酸2～8min,一日孵化率都在95%以上;保护时间或达到25h时,如果盐酸刺激量不足,一日孵化率则呈现降低的趋势;保护时间达到30h时,盐酸刺激量强弱不能把蚕种一日孵化率提高到92%以上;从整体来看,浸酸时间达到5min以后,盐酸刺激量越大,蚕卵一日孵化率越小,蚕卵一日孵化率占实用孵化率的比例也越小。